In this paper, glass fiber felts are fabricated by centrifugal-spinneret-blow process. Swing cylinder is designed to obtain a micro-layer structure, and the phase difference of two swing cylinders is π/2 + 2kπ. Tensile strength, flexural rigidity, and thermal conductivity of various glass fiber felts are investigated. The experimental results indicate that the tensile strength of micro-layer glass fiber felts and random glass fiber felts is 0.015 MPa and 0.013 MPa, respectively. In addition, the tensile strength of glass fiber felts is also improved with the increase of the density and the resin content of glass fiber felts. The micro-layer structure decreases the flexural rigidity of glass fiber felts, and the flexural rigidity of glass fiber felts with micro-layer and random structures is 43.4 g.cm and 101.3 g.cm, respectively. The mean thermal conductivity of glass fiber felts with micro-layer and random structures is 31.57 mW/m·k and 35.69 mW/m·k, respectively.