A combined molecular biology and network pharmacology approach to investigate the multi-target mechanisms of Chaihu Shugan San on Alzheimer’s disease

Zeng, Qiang; Li, Longfei; Siu, Wing Tai; Jin, Yu; Cao, Meiqun; Liu, Weifeng; Chen, Jian; Cong, Weihong; Ma, Min; Chen, Ke-ji; Wu, Zhengzhi

doi:10.1016/j.biopha.2019.109370

Cited by 72 publications

(46 citation statements)

References 54 publications

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“…Compounds showing DL property are not drugs but have the potential to become a drug [29]. A DL value above 0.18 indicates that the compound is chemically suitable for drug development [30]. Therefore, according to the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) (TCMSP, http://tcmspw.com/tcmsp.php, version 2.3) criteria, the compounds with OB ≥ 30% and DL ≥ 0.18 were selected as potential active components for further analysis [19].…”

Section: Filtering Strategy For Componentsmentioning

confidence: 99%

“…This database provides a platform for exploring known interactions between small molecules and proteins and for protein-protein interactions according to organisms [2,31]. The STITCH confidence score is used to define a set of high-confidence interactions between chemical compounds and proteins [30]. In this study, a final list of genes linked to different chemical components (with a confidence score ≥ 0.7, indicating a high confidence score according to STITCH) were obtained [32].…”

Section: Target Genesmentioning

confidence: 99%

“…In this study, a final list of genes linked to different chemical components (with a confidence score ≥ 0.7, indicating a high confidence score according to STITCH) were obtained [32]. Gene information, including gene ID and name, was verified in the UniProt database by limiting species to "Homo sapiens" [24,30].…”

Section: Target Genesmentioning

confidence: 99%

“…Functional annotation analysis of the selected genes from the PPI network was performed using DAVID. KEGG pathways with p value < 0.05 were considered statistically significant [29,30].…”

Section: Signaling Pathway Analysesmentioning

confidence: 99%

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Pharmacological Effects of Agastache rugosa against Gastritis Using a Network Pharmacology Approach

et al. 2020

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Agastache rugosa is used as a Korean traditional medicine to treat gastric diseases. However, the active ingredients and pharmacological targets of A. rugosa are unknown. In this study, we aimed to reveal the pharmacological effects of A. rugosa on gastritis by combining a mice model and a network pharmacology method. The macrophage and gastritis-induced models were used to evaluate the pharmacological effects of A. rugosa. The results show that A. rugosa relieved mucosal damage induced by HCl/EtOH in vivo. Network analysis identified 99 components in A. rugosa; six components were selected through systematic screening, and five components were linked to 45 gastritis-related genes. The main components were acacetin and luteolin, and the identified core genes were AKT serine/threonine kinase 1 (AKT1), nuclear factor kappa B inhibitor alpha (NFKBIA), and mitogen-activated protein kinase-3 (MAPK3) etc. in this network. The network of components, target genes, protein–protein interactions, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway was closely connected with chemokines and with phosphoinositide 3-kinase-Akt (PI3K/AKT), tumor-necrosis-factor alpha (TNFα), mitogen-activated protein kinase, nuclear factor kappa B, and Toll-like receptor (TLR) pathways. In conclusion, A. rugosa exerts gastro-protective effects through a multi-compound and multi-pathway regulatory network and holds potential for treating inflammatory gastric diseases.

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Section: Filtering Strategy For Componentsmentioning

confidence: 99%

Section: Target Genesmentioning

confidence: 99%

Section: Target Genesmentioning

confidence: 99%

“…Functional annotation analysis of the selected genes from the PPI network was performed using DAVID. KEGG pathways with p value < 0.05 were considered statistically significant [29,30].…”

Section: Signaling Pathway Analysesmentioning

confidence: 99%

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Pharmacological Effects of Agastache rugosa against Gastritis Using a Network Pharmacology Approach

et al. 2020

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“…"Non-alcoholic fatty liver disease" was used as the keyword and duplicate values were deleted. The Bioconductor package in the software package R was used to standardize the disease gene targets obtained (Zeng et al, 2019). Finally, Cytoscape 3.2.1 was used to perform visual network analysis of the "disease-target.…”

Section: Compound Profiling and Disease Target Identificationmentioning

confidence: 99%

Veratrilla baillonii Franch Could Alleviate Lipid Accumulation in LO2 Cells by Regulating Oxidative, Inflammatory, and Lipid Metabolic Signaling Pathways

Huang

Liang

et al. 2020

Front. Pharmacol.

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Based on the pathological theory of lipid metabolism and using network pharmacology, this study was designed to investigate the protective effect of water extract of Veratrilla baillonii (WVBF) on non-alcoholic fatty liver disease (NAFLD) model using LO2 cells and to identify the potential mechanism underlying the effect. The components of V. baillonii were identified from the public database of traditional Chinese medicine systems pharmacology database (TCMSP). Cytoscape software was used to construct the related composite target network. Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were carried out for critical nodes. The BioGPS database was used to determine the distribution of the target in tissues and organs. Moreover, the inhibitory effect of V. baillonii was further investigated using an in vitro hepatocyte NAFLD model. Fourteen active components were then selected from the 27 known compounds of V. baillonii. The targets of gene enrichment analysis were mainly distributed in the lipid catabolism-related signaling pathway. Network analysis revealed that five target genes of TNF, MAPK8, mTOR, NF-ĸB, and SREBP-1c were key nodes and played important roles in this process. Organ localization analysis indicated that one of the core target site of V. baillonii was liver tissue. The results of the in vitro study revealed that WVBF can alleviate the inflammatory response and lipid accumulation in LO2 hepatocytes by inhibiting oxidative stress and the adipocytokine signaling pathway. Genes and proteins related to the lipid synthesis, such as SREBP-1C, acetyl-CoA carboxylase (ACC), and fatty acid synthase (FASN), were significantly decreased, and PPARa expression is significantly increased with WVBF administration. In conclusion, V. baillonii may regulate local lipid metabolism and attenuate oxidative stress and inflammatory factors through the PPARa/ SREBP-1c signaling pathway. The present study also indicates that multiple components

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Traditional Chinese medicine for dementia

2021

Alzheimer's & Dementia

Self Cite

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In Western medicine, dementia refers to a spectrum of diseases affecting cognition, mental health, and physical abilities. Ancient medical literatures of traditional Chinese medicine (TCM) also recorded dementia-like symptoms, but described the disease from a totally distinct theoretical point of view. The earliest records of memory loss in ancient China can be traced back 2000 years earlier. In TCM, dementia can be attributed mainly to the Brain dystrophy, Spleen-Kidney weakness, Blood stasis, and Phlegm stagnation. Of interest, ancient Chinese physicians have proposed that dementia manifests as not only cognitive but also noncognitive symptoms including psychiatric disorders and sleep disturbance, which have been investigated widely nowadays in Western medicine. Various TCM prescriptions, herbal medicines, and acupunctures have also been proposed for dementia prevention and therapy. Some of these strategies are still used in current clinical practice. Reviewing and highlighting the unique TCM recognition of treating dementia may shed light on future dementia research.

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A combined molecular biology and network pharmacology approach to investigate the multi-target mechanisms of Chaihu Shugan San on Alzheimer’s disease

Cited by 72 publications

References 54 publications

Pharmacological Effects of Agastache rugosa against Gastritis Using a Network Pharmacology Approach

Pharmacological Effects of Agastache rugosa against Gastritis Using a Network Pharmacology Approach

Veratrilla baillonii Franch Could Alleviate Lipid Accumulation in LO2 Cells by Regulating Oxidative, Inflammatory, and Lipid Metabolic Signaling Pathways

Traditional Chinese medicine for dementia

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