BACKGROUND Heat-polymerized acrylic resins are commonly used as denture base, but they have relatively low mechanical properties such as impact strength, transverse strength, and modulus of elasticity that made them susceptible to fracture during clinical application. Various modifications were made to improve the mechanical properties of heat-polymerized acrylic resins, one of them was with the addition of E-glass fiber. We wanted to determine the effect of E-glass fiber (chopped strands of 3 mm size) with 1% and 1.5% weight concentration addition on mechanical properties (impact strength, transverse strength, and modulus of elasticity) of heatpolymerized acrylic resin denture base material and the correlation between them. MATERIALS AND METHODS Ninety samples of heat-polymerized acrylic resin were divided into 9 groups, consisting of impact strength, transverse strength and modulus of elasticity testing group. Each testing group consisting of group without and with addition of 1% and 1.5% E-glass fiber. Impact strength test was performed using Charpy impact tester, while transverse strength and modulus of elasticity were performed using the Universal Testing Machine. After the test, fracture surface of the samples was evaluated and scanning electron microscope view was taken. The effect of E-glass fiber addition to impact, transverse strength and modulus of elasticity on heatpolymerized acrylic resin was analysed with one-way ANOVA statistical test. Pearson Correlation test was then performed to determine the correlation between impact, transverse strength and modulus of elasticity. RESULTS There was an effect of E-glass fiber addition (chopped strands of 3 mm size) with 1% and 1.5% weight concentration to impact strength p=0.0001 (p<0,05); transverse strength p=0,002 (p<0,05); and modulus of elasticity p=0.002 (p<0.05). Pearson Correlation test showed that there was no correlation between impact and transverse strength (r=0.311 and p=0.094) (p>0.05); impact strength and modulus of elasticity (r=0.284 and p=0.128) (p>0.05); and transverse strength with modulus of elasticity (r=-0.044 and p=0.816) (p>0.05). CONCLUSION Addition of 1% and 1.5% E-glass fiber can improve the impact strength, transverse strength, and modulus of elasticity of heatpolymerized acrylic resin denture base and the addition of 1% E-glass fiber can increase a balanced of impact strength, transverse strength, and modulus of elasticity of heat-polymerized acrylic resin denture base.