A comparative study of two methods of dental pulp extraction for genetic fingerprinting

Tilotta, Françoise; Brousseau, Philippe; Lepareur, Elise; Yasukawa, Kazutoyo; Mazancourt, Philippe de

doi:10.1016/j.forsciint.2010.06.019

Cited by 15 publications

(6 citation statements)

References 22 publications

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“…In agreement with (Tilotta et al, 2010), the pulp was successfully extirpated in the current study by a standard endodontic access. The main advantage of this method is being conservative, so the tooth can be used for further investigation.…”

Section: Discussionsupporting

confidence: 75%

“…In sectioning the teeth, the access to the pulp chamber, by clear fracture, needs an experienced dentist. It is also difficult to perform teeth sectioning in case of pulp retraction (Tilotta et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…The main advantage of this method is being conservative, so the tooth can be used for further investigation. It is also performed under air and water spray to prevent heating, whereas crushing or sectioning produces heat which may damage the genetic material (Tilotta et al, 2010). However, the endodontic access method utilized in this study required the need for a skilled dentist where the pulp was extracted with sufficient quality and quantity even from elder teeth.…”

Section: Discussionmentioning

confidence: 99%

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Sex determination from dental pulp DNA among Egyptians

Kholief

El-Shanawany

Gomaa

2017

Egypt J Forensic Sci

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Background: A great challenge arises when sex determination of human remains is needed, especially when only, bone fragments or isolated teeth are available; such as in mass disasters. Thus, the aim of the present work is to extract and analyze the DNA from dental pulp for sex determination of a sample of Egyptian population using the polymerase chain reaction amplification of DYS14 and SRY genes. Materials and methods: DNA was extracted from the dental pulp of forty freshly extracted teeth (sound and carious) collected from adult Egyptians of both sexes using QIAamp® DNA Investigator Kit. Androgen Receptor gene was PCR amplified as an internal positive control. DYS14 and SRY genes were PCR amplified for sex determination. Results: DNA extraction was confirmed by detection of the amplified AR gene band (292 bp) in all samples. Sex was successfully determined for all the studied teeth as revealed by amplification of 158 bp band, 778 bp band of the DYS14 and SRY genes respectively. Conclusion: DYS14 and SRY genes were found to be reliable for dental sex determination regardless of the condition of teeth, whether sound or carious.

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Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Sex determination from dental pulp DNA among Egyptians

Kholief

El-Shanawany

Gomaa

2017

Egypt J Forensic Sci

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“…Primary cells were isolated and cultured from the extracted impacted molars as previously described by three authors. [11][12][13] Briefly, HDP tissues from the pulp chamber and the root canal were collected and then digested with 0.3 mg collagenase type II (SERVA, Germany) enzyme for 2 hours at 37°C. Then, the cell strainer (70-μm) was infiltrated for the suspension of the single cell and the centrifugation at 60 g for 8 minutes was used for the collection of the cells which were suspended in the plating medium.…”

Section: Methodsmentioning

confidence: 99%

Efficacy of Photobiomodulation and Vitamin D on Odontogenic Activity of Human Dental Pulp Stem Cells

et al. 2021

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Introduction: The regeneration of dental pulp tissue using human dental pulp stem cells (HDPSCs) has attracted increasing attention in recent years. Recent studies have suggested that several factors such as photobiomodulation (PBM) and vitamin D affect the proliferation and differentiation of HDPSCs. Therefore, the present study evaluated the effects of PBM and vitamin D on odontogenic differentiation of HDPSCs for dentin -like tissue formation. Methods: HDPSCs were collected, isolated, and characterized and then divided into six groups: group I, control; group II, vitamin D (10-7 Mol); group III, irradiation at 1 J/cm2 of 810 nm diode laser; group IV, irradiation at 1 J/cm2 and culture with vitamin D; group V, irradiation at 2 J/cm2 , and group VI, irradiation at 2 J/cm2 and culture with vitamin D, cell viability assay was measured through MTT. Alkaline phosphatase (ALP) enzyme activity and mRNA levels of vascular endothelial growth factor (VEGF), bone morphogenic protein-2 (BMP-2), and dentin sialophosphoprotein (DSPP) were also assessed. Results: PBM at 1 and 2 J/cm2 combined with vitamin D significantly promoted HDPSCs proliferation through MTT assay and odontogenic differentiation through gene expression of VEGF, BMP-2, and DSPP levels (P<0.0001). Conclusion: PBM at 2 J/cm2 combined with vitamin D enhanced the HDPSCs proliferation and odontogenic differentiation and thus could be a novel strategy for dentin regeneration in dentistry.

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“…Perhaps the effects of temperature are more pronounced over longer time frames. Tilotta et al (24) compared the nuclear DNA from teeth crushed to those in which the pulp remnants were sampled via regular endodontic access and noted a significantly higher yield from the teeth sampled via the more conservative technique. In this instance, 90% and 70% success rates for mtDNA and nuclear DNA recovery from teeth, respectively, dating back to 300 BC and 1600 AD were reported.…”

Section: Introductionmentioning

confidence: 99%

The Effect of EDTA Buffer and Temperature on DNA Extraction from Teeth for Molecular Forensic Assessment

Nasrollahzadehsabet

Esmeilzadeh

Shirmohammady

et al. 2021

Ann Mil Health Sci Res

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: Using DNA to generate genetic profiles of individuals is an efficient and accurate technique. Achieving the right and net amount of DNA is one of the challenges in this area. Due to tissues destruction after death, it is usually very difficult to achieve proper DNA. So, the use of hard tissues such as bones and teeth as important resources can help in these cases. Accordingly, the use of ion chelating buffers is one of the most important parts of preparing these tissues to extract DNA. In this study, a buffer containing ethylenediaminetetraacetic acid (EDTA) buffer (0.5 mM) and distilled water was used as a control. Different temperatures were also examined. The average concentration of DNA extracted from the sample into ternary sort at a temperature of 55°C, 37°C, 22°C, and 4°C was equal to 19.68 ng/µL, 12.23 ng/µL, 17.19 ng/µL, and 15.06 ng/µL, respectively. For evaluation, sterile distilled water was used instead of buffer, which was equal to 7.9 ng/µL at 55°C. Based on the results of this study, the buffer containing EDTA was found to be suitable for releasing genomic resources from bones and teeth.

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A comparative study of two methods of dental pulp extraction for genetic fingerprinting

Cited by 15 publications

References 22 publications

Sex determination from dental pulp DNA among Egyptians

Sex determination from dental pulp DNA among Egyptians

Efficacy of Photobiomodulation and Vitamin D on Odontogenic Activity of Human Dental Pulp Stem Cells

The Effect of EDTA Buffer and Temperature on DNA Extraction from Teeth for Molecular Forensic Assessment

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