A comparative study of various physicochemically modified capillaries used in CE technique for the three distinct analytical purposes

Nowak, Paweł Mateusz; Woźniakiewicz, Michał; Garnysz, Magdalena; Kościelniak, Paweł

doi:10.1016/j.jchromb.2016.03.037

Cited by 3 publications

(4 citation statements)

References 32 publications

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“…This effect was not observed with the other capillaries, suggesting that the interaction between HSA and “unprotected” silanol groups may be the main obstacle hampering analysis. A similar effect was observed by us in our recent work, when HSA was used as an p K a -shift inducer [ 33 ].…”

Section: Resultssupporting

confidence: 87%

“…In conclusion, for this type of sample, the dynamic coating performed with the silica capillary is appreciably better than any other capillary type. In reference to the other published works [ 33 , 36 , 37 ], the effectiveness of the amine- and diol-modified capillaries in comparison to the bare silica capillary occurred to be noticeably worse. The dynamically coated silica capillary, contrary to the previous observations [ 37 ], turned out to be more effective in the stabilization of migration times than the dynamically coated amine capillary.…”

Section: Resultsmentioning

confidence: 73%

“…On the other hand, the use of any type of coating significantly elevates the cost of analysis. The direct comparisons of various types of capillary modifications are rarely performed [ 27 , 29 , 33 ]. On that account, there is still little known about advantages and predispositions of the given capillary types in regard to the particular types of methods and experimental conditions, and this hinders their critical evaluation and selection.…”

Section: Introductionmentioning

confidence: 99%

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Improving repeatability of capillary electrophoresis—a critical comparison of ten different capillary inner surfaces and three criteria of peak identification

et al. 2017

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A poor repeatability of migration times caused by the fluctuations of electroosmotic flow (EOF) is an inherent weakness of capillary electrophoresis. Most researchers endeavor to prevent this problem using relative migration times or various capillary coatings which are expensive and not easy in comparison. Herein, we present an original approach to this problem: we apply a model sample designed to induce significant EOF instability, in order to critically compare ten capillary types with different physicochemical characteristics. Moreover, we accompany capillary modification with the evaluation of various criteria of peak identification: migration time, migration times ratio, and electrophoretic mobility. Our results show a great effectiveness of a dynamic coating in the stabilization of migration times, with the average RSD(%) value reduced from 3.5% (bare silica capillary) down to 0.5%. The good outcomes were also obtained for the surfactant-modified silica and amine capillaries. For the capillaries exhibiting significant instability of EOF, electrophoretic mobility turned out to be a more universal and reliable criterion of peak identification than relative migration time. It can be explained by an intrinsic dependency of migration times ratio on EOF change, which should always be considered during the selection of an internal standard.Electronic supplementary materialThe online version of this article (doi:10.1007/s00216-017-0382-y) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

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Improving repeatability of capillary electrophoresis—a critical comparison of ten different capillary inner surfaces and three criteria of peak identification

et al. 2017

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“…The broad commercial availability of permanently coated capillaries and reagents for reversible dynamic coating makes the choice not straightforward. Intriguingly, only several systematic comparisons of various capillary modifications can be found in the literature [6–13]. …”

Section: Introductionmentioning

confidence: 99%

Capillary coating as an important factor in optimization of the off-line and on-line MEKC assays of the highly hydrophobic enzyme chlorophyllase

et al. 2016

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The choice between bare and coated capillaries is a key decision in the development and use of any methods based on capillary electrophoresis. In this work several permanently and dynamically coated capillaries were successfully implemented in a previously developed micellar electrokinetic chromatography (MEKC) assay of the plant membrane enzyme chlorophyllase. The results obtained demonstrate the rationale behind the use of capillary coating, which is crucial for successful optimization of both the off-line mode and the on-line/electrophoretically mediated microanalysis assay mode. The application of an amine permanently coated capillary (eCAP) is a simple way to significantly increase the repeatability of migration times and peak areas, and to ensure a strong electroosmotic flow that considerably decreases the overall analysis time. A dynamic coating (CEofix) allows one to apply an on-line incubation to control the reaction progress inside the capillary, and to increase the signal-to-noise ratio and peak efficiency. The dynamic coating is possible with use of both the normally applied uncoated silica capillary and the precoated amine capillary, which ensures more repeatable migration times. The strong points of the uncoated silica capillary are its attractive price and wide range of pH that can be applied. The characteristics presented may simplify the choice of capillary modification, especially in the case of hydrophobic analytes, MEKC-based separations, and other enzymatic assays.Electronic supplementary materialThe online version of this article (doi:10.1007/s00216-016-0097-5) contains supplementary material, which is available to authorized users.

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Acidity of substituted cathinones studied by capillary electrophoresis using the standard and fast alternative approaches

Woźniakiewicz

Nowak

Gołąb

et al. 2018

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A comparative study of various physicochemically modified capillaries used in CE technique for the three distinct analytical purposes

Cited by 3 publications

References 32 publications

Improving repeatability of capillary electrophoresis—a critical comparison of ten different capillary inner surfaces and three criteria of peak identification

Improving repeatability of capillary electrophoresis—a critical comparison of ten different capillary inner surfaces and three criteria of peak identification

Capillary coating as an important factor in optimization of the off-line and on-line MEKC assays of the highly hydrophobic enzyme chlorophyllase

Acidity of substituted cathinones studied by capillary electrophoresis using the standard and fast alternative approaches

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