A Comparison of Electrospun Polymers Reveals Poly(3-Hydroxybutyrate) Fiber as a Superior Scaffold for Cardiac Repair

CastellanoDelia,; BlanesMaría,; MarcoBruno,; CerradaInmaculada,; Ruiz-SauríAmparo,; PelachoBeatriz,; Araña, Miriam; MonteroJose, A; CambraVicente,; Prósper, Felipe; SepúlvedaPilar,

doi:10.1089/scd.2013.0578

Cited by 81 publications

(63 citation statements)

References 44 publications

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“…Macrophages already activated by inflammatory signals will then respond to IL-4 and IL-13 by fusing to form multinucleated FBGCs. Both IL-4 and IL-13 have been shown to be secreted in larger quantities by macrophages cultured on electrospun silk scaffolds in vitro (24). Not surprisingly therefore, the implantation of silk materials often induces the formation of FBGCs.…”

Section: Mechanism Of Silk Degradationmentioning

confidence: 99%

In vivo bioresponses to silk proteins

2015

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Silks are appealing materials for numerous biomedical applications involving drug delivery, tissue engineering, or implantable devices, because of their tunable mechanical properties and wide range of physical structures. In addition to the functionalities needed for specific clinical applications, a key factor necessary for clinical success for any implanted material is appropriate interactions with the body in vivo. This review summarizes our current understanding of the in vivo biological responses to silks, including degradation, the immune and inflammatory response, and tissue remodeling with particular attention to vascularization. While we focus in this review on silkworm silk fibroin protein due to the large quantity of in vivo data thanks to its widespread use in medical materials and consumer products, spider silk information is also included if available. Silk proteins are degraded in the body on a time course that is dependent on the method of silk fabrication and can range from hours to years. Silk protein typically induces a mild inflammatory response that decreases within a few weeks of implantation. The response involves recruitment and activation of macrophages and may include activation of a mild foreign body response with the formation of multinuclear giant cells, depending on the material format and location of implantation. The number of immune cells present decreases with time and granulation tissue, if formed, is replaced by endogenous, not fibrous, tissue. Importantly, silk materials have not been demonstrated to induce mineralization, except when used in calcified tissues. Due to its ability to be degraded, silk can be remodeled in the body allowing for vascularization and tissue ingrowth with eventual complete replacement by native tissue. The degree of remodeling, tissue ingrowth, or other specific cell behaviors can be modulated with addition of growth or other signaling factors. Silk can also be combined with numerous other materials including proteins, synthetic polymers, and ceramics to enhance its characteristics for a particular function. Overall, the diverse array of silk materials shows excellent bioresponses in vivo with low immunogenicity and the ability to be remodeled and replaced by native tissue making it suitable for numerous clinical applications.

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Section: Mechanism Of Silk Degradationmentioning

confidence: 99%

In vivo bioresponses to silk proteins

2015

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“…Recently Castellano et al presented an extensive study on the ability for cardiac repair of various electrospun scaffolds including poly(3-hydroxybutyrate) (PHB), PCL, silk, PLLA, polyamide (PA) which were compared to a non-crosslinked collagen membrane that was used as control [114]. In vitro studies using mouse cardiac HL-1 cell line, cardiac fibroblasts and MSCs from dental pulp showed that PHB and PCL polymers allowed the greatest adhesion/growth of cells.…”

Section: Scaffolds Based On Other Polymeric Materialsmentioning

confidence: 99%

“…Plasma treated samples become more hydrophilic, allowing the attachment of cardiac cells as well as the desired proliferation and shape. Likewise Castellano et al utilized N 2 plasma for the introduction of polar groups onto the surface of PHB, PCL, PLLA, silk, PA electrospun scaffolds prior to cell culture [114].…”

Section: Post Treatment By Plasma Modificationmentioning

confidence: 99%

Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering

et al. 2017

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Please cite this article as: Kitsara, M., Agbulut, O., Kontziampasis, D., Chen, Y., Menasché, P., Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering, Acta Biomaterialia (2016), doi: http://dx.doi.org/ 10. 1016/j.actbio.2016.11.014 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractCardiac cell therapy holds a real promise for improving heart function and especially of the chronically failing myocardium. Embedding cells into 3D biodegradable scaffolds may better preserve cell survival and enhance cell engraftment after transplantation, consequently improving cardiac cell therapy compared with direct intramyocardial injection of isolated cells.The primary objective of a scaffold used in tissue engineering is the recreation of the natural 3D environment most suitable for an adequate tissue growth. An important aspect of this commitment is to mimic the fibrillar structure of the extracellular matrix, which provides essential guidance for cell organization, survival, and function. Recent advances in nanotechnology have significantly improved our capacities to mimic the extracellular matrix. Among them, electrospinning is well known for being easy to process and cost effective. Consequently, it is becoming increasingly popular for biomedical applications and it is most definitely the cutting edge technique to make scaffolds that mimic the extracellular matrix for industrial applications.Here, the desirable physico-chemical properties of the electrospun scaffolds for cardiac therapy are described, and polymers are categorized to natural and synthetic. Moreover, the methods used for improving functionalities by providing cells with the necessary chemical cues and a more in vivo-like environment are reported.

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“…For instance, in the work of Castellano D. et al, collagen, poly(3-hydroxybutyrate), poly(ecaprolactone), poly-lactic acid and polyamide NFs were generated by electrospinning, being then transplanted into a rat MI model. Interestingly, poly(3-hydroxybutyrate) was the scaffold with the most beneficial reparative potential and positive remodeling capacity [85]. In another study polyester urethane urea NFs showed suitable mechanical properties and biocompatible characteristics, allowing cellular integration and endocardial endothelialization with minimal inflammation [86].…”

Section: Nanofibersmentioning

confidence: 99%

Heart regeneration after myocardial infarction using synthetic biomaterials

Pascual-Gil

Garbayo

Díaz-Herráez

et al. 2015

Journal of Controlled Release

122

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Abstract:Myocardial infarction causes almost 7.3 million deaths each year worldwide. However, current treatments are more palliative than curative. Presently, cell and protein therapies are considered the most promising alternative treatments. Clinical trials performed until now have demonstrated that these therapies are limited by protein short half-life and by low transplanted cell survival rate, prompting the development of novel cell and protein delivery systems able to overcome such limitations. In this review we discuss the advances made in the last 10 years in the emerging field of cardiac repair using biomaterial-based delivery systems with focus on the progress made on preclinical in vivo studies. Then, we focus in cardiac tissue engineering approaches, and how the incorporation of both cells and proteins together into biomaterials has opened new horizons in the myocardial infarction treatment. Finally, the ongoing challenges and the perspectives for future work in cardiac tissue engineering will also be discussed.

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A Comparison of Electrospun Polymers Reveals Poly(3-Hydroxybutyrate) Fiber as a Superior Scaffold for Cardiac Repair

Cited by 81 publications

References 44 publications

In vivo bioresponses to silk proteins

In vivo bioresponses to silk proteins

Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering

Heart regeneration after myocardial infarction using synthetic biomaterials

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