A comparison of fibrin sealants in relation to their in vitro and in vivo properties

Dickneite, Gerhard; Metzner, Hubert J.; Pfeifer, Thomas; Kroez, Monika; Witzke, Günther

doi:10.1016/j.thromres.2003.10.010

Cited by 76 publications

(64 citation statements)

References 23 publications

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“…Isolation techniques (eg, cryoprecipitation and ethanol precipitation), batch to batch variability, and composition (eg, concentration of thrombin, fibrinogen, factor XIII, and antifibrolytics) lead to different mechanical and biological properties of the generated clots. 16,17 For example, Dickneite et al showed clear differences in hemostatic and clot strength properties of 12 commercially available fibrin sealants. 17 Furthermore, differences in component origin may change the biochemistry of the clot, like interspecies or pooled and single donor.…”

Section: Difficulties Involved In Assessing the Literaturementioning

confidence: 99%

“…16,17 For example, Dickneite et al showed clear differences in hemostatic and clot strength properties of 12 commercially available fibrin sealants. 17 Furthermore, differences in component origin may change the biochemistry of the clot, like interspecies or pooled and single donor. 18,19 Variations in experimental design, implantation site, and animal models as well as fibrinogen and thrombin concentrations are other parameters that make direct comparison of the research findings difficult.…”

Section: Difficulties Involved In Assessing the Literaturementioning

confidence: 99%

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A review of fibrin and fibrin composites for bone tissue engineering

Noori

Ashrafi

Vaez-Ghaemi

et al. 2017

IJN

377

256

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Tissue engineering has emerged as a new treatment approach for bone repair and regeneration seeking to address limitations associated with current therapies, such as autologous bone grafting. While many bone tissue engineering approaches have traditionally focused on synthetic materials (such as polymers or hydrogels), there has been a lot of excitement surrounding the use of natural materials due to their biologically inspired properties. Fibrin is a natural scaffold formed following tissue injury that initiates hemostasis and provides the initial matrix useful for cell adhesion, migration, proliferation, and differentiation. Fibrin has captured the interest of bone tissue engineers due to its excellent biocompatibility, controllable biodegradability, and ability to deliver cells and biomolecules. Fibrin is particularly appealing because its precursors, fibrinogen, and thrombin, which can be derived from the patient’s own blood, enable the fabrication of completely autologous scaffolds. In this article, we highlight the unique properties of fibrin as a scaffolding material to treat bone defects. Moreover, we emphasize its role in bone tissue engineering nanocomposites where approaches further emulate the natural nanostructured features of bone when using fibrin and other nanomaterials. We also review the preparation methods of fibrin glue and then discuss a wide range of fibrin applications in bone tissue engineering. These include the delivery of cells and/or biomolecules to a defect site, distributing cells, and/or growth factors throughout other pre-formed scaffolds and enhancing the physical as well as biological properties of other biomaterials. Thoughts on the future direction of fibrin research for bone tissue engineering are also presented. In the future, the development of fibrin precursors as recombinant proteins will solve problems associated with using multiple or single-donor fibrin glue, and the combination of nanomaterials that allow for the incorporation of biomolecules with fibrin will significantly improve the efficacy of fibrin for numerous bone tissue engineering applications.

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Section: Difficulties Involved In Assessing the Literaturementioning

confidence: 99%

Section: Difficulties Involved In Assessing the Literaturementioning

confidence: 99%

A review of fibrin and fibrin composites for bone tissue engineering

Noori

Ashrafi

Vaez-Ghaemi

et al. 2017

IJN

377

256

View full text Add to dashboard Cite

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“…Fibrin glues (FGs) are mainly exploited from pooled plasma and consist of different amounts of purified and virally inactivated human proteins: fibrinogen, thrombin, factor XIII, anti-fibrinolytic agents, and calcium chloride. Fibrin scaffolds can be used as a principal element in TE approaches and provide complete autologous construction (Dickneite et al 2003;Montana et al 2012). Alginate is shown as a nontoxic hydrogel scaffold and hence has been used in TE for culturing of chondrocytes (Ning 2011).…”

Section: Introductionmentioning

confidence: 99%

The effects of synthetic and natural scaffolds on viability and proliferation of adipose-derived stem cells

Ghiasi

Kalhor

Qomi

et al. 2015

Frontiers in Life Science

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This study presents a comparative assessment of adipose-derived stem cell (ADSCs) proliferation rates and their viability on five different scaffolds. Five different biomaterial scaffolds were prepared: alginate, poly lactic-co-glycolic acid, fibrin glue, inactive platelet-rich plasma, and active plateletrich plasma (APRP). Stem cells were isolated from human adipose tissue. Flow cytometry analysis was performed. Specifically, adipogenesis/osteogenesis/chondrogenesis-associated genes expression was analyzed by real-time polymerase chain reaction. These cells were seeded in the prepared scaffolds. After 14 days, the proliferation and viability of MSCs were evaluated using an MTT assay. Also, stemness genes expression was analyzed with the reverse transcriptasepolymerase chain reaction (RT-PCR) method. In addition, the DNA content assay was also performed. The obtained results showed a significant difference between cell proliferation and viability of different scaffolds. APRP and alginate were shown to be the most and least suitable scaffolds in terms of enhancing cell proliferation and maintaining cell viability respectively (p < .05). RT-PCR reactions demonstrated the expression of the various stemness-related markers (Nanog, Octamer4A, and Sox2) when ADSC cells were grown separately on the five different scaffolds. Our study indicates that compared with the scaffolds, APRP could be the best scaffold for support of ADSC proliferation. ARTICLE HISTORY

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“…In commercial sealants the thrombin activity has been reported at 4-1000 IU/ml [4,5] and the fibrinogen concentration at 19.88 mg/ml, 35-55 mg/ml and 80-100 mg/ml [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…The adhesive properties of fibrin glue preparations are used to classify the products and are often evaluated via tensile testing [1,[5][6][7]. Tensile testing of fibrin glue has been reported using vital human tissue [6], rat or pig skin and canine or rat models [7].…”

Section: Introductionmentioning

confidence: 99%

Autologous fibrin glue: automated production and adhesive quality

Düregger

Frenzel

Eblenkamp

2017

Current Directions in Biomedical Engineering

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Fibrin glue is a two-component adhesive used to stop bleeding, seal wound edges and for scaffolds in tissue engineering. Autologous products result in reduced risk of contamination and immunological responses compared to commercially available fibrin glue. However, reproducibility due to patient dependent sealant properties of autologous fibrin glue preparation is low. In this study a fully automated production process for both the fibrinogen and thrombin component from small blood volumes was developed. The resulting fibrinogen concentration, thrombin activity and sealant properties of the fibrin glue were determined. The fully automated and closed production system proved to be a promising tool for fast and easy production of autologous fibrin glue with an effective adhesive quality.

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A comparison of fibrin sealants in relation to their in vitro and in vivo properties

Cited by 76 publications

References 23 publications

A review of fibrin and fibrin composites for bone tissue engineering

A review of fibrin and fibrin composites for bone tissue engineering

The effects of synthetic and natural scaffolds on viability and proliferation of adipose-derived stem cells

Autologous fibrin glue: automated production and adhesive quality

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