Autologous fibrin glue: automated production and adhesive quality

Abstract

Fibrin glue is a two-component adhesive used to stop bleeding, seal wound edges and for scaffolds in tissue engineering. Autologous products result in reduced risk of contamination and immunological responses compared to commercially available fibrin glue. However, reproducibility due to patient dependent sealant properties of autologous fibrin glue preparation is low. In this study a fully automated production process for both the fibrinogen and thrombin component from small blood volumes was developed. The resulting fibrinogen concentration, thrombin activity and sealant properties of the fibrin glue were determined. The fully automated and closed production system proved to be a promising tool for fast and easy production of autologous fibrin glue with an effective adhesive quality.

Injectable Phenolic-Chitosan Self-Healing Hydrogel with Hierarchical Micelle Architectures and Fast Adhesiveness

“…The adhesiveness of a hydrogel or a glue relies heavily on the balance and synergy of both cohesion and adhesion . A variety of strategies have been utilized to enhance the adhesiveness, including mussel-inspired chemistry or the nanostructure approach. , Mussel-inspired hydrogels with different solid contents exhibited a binding strength from 2 to 210 kPa on porcine skin according to various testing methods, while the average binding strength of the commercial fibrin glue was in the range 5.31–8.1 kPa . In this study, CPF hydrogel may possess adhesion from phenolic chemistry and cohesion from the hierarchical structure.…”

Role of Autologous Fibrin Glue (AFG) to Reduce Drainage Volume After Modified Radical Mastectomy for Breast Cancer

“…Blood processing was performed with a standard laboratory centrifuge (Rotanta 460, Andreas Hettich GmbH Co KG; Tuttlingen, Germany) equipped with a specially designed rotor for automated production of autologous blood derivatives. All photometric measurements were performed with a microplate photometer (Multiclan FC, Thermo Fisher Scientific, Waltham, MA, USA) [9].…”

“…In the first centrifugation step at 930 g for five minutes, platelet-rich plasma (PRP), or for 20 minutes, plateletpoor plasma (PPP), respectively, was generated in syringe A. Plasma was then transferred into syringe B and mixed with the prefilled ethanol [9].…”

“…[50] All produced fibrinogen was mixed with 2 ml of fibrinogen reagent (116 g/l ammonium sulfate, 1.4 g/l guanidine hydrochloride, 9 g/l EDTA, deionized water, pH value of 4.9). Photometric measurements were performed after 120 sec at 405 nm and compared to measurements with human plasma fibrinogen (341576-100MG, Merck Millipore Corp., Billerica, MA, USA) of known concentration (25 mg/ml) (9). The sealant quality was determined by mixing the thrombin and fibrinogen components at equal volumes and evaluating the clotting properties via clot formation size of the formed clot and adhesive strength.…”

“…We inserted two separated vacuum drains at the axilla and under the skin flap at both groups. We removed the drains after draining fluid volume less than 30 cc per 24 hours [9,10].…”