A comparison of fuel preferences of mitochondria from vertebrates and invertebrates

Moyes, Christopher D.; Suarez, Raul K.; Hochachka, Peter W.; Ballantyne, James S.

doi:10.1139/z90-201

Cited by 47 publications

(18 citation statements)

References 151 publications

(155 reference statements)

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“…As discussed by Jones et al (2008) and Viant et al (2003), some marine mollusks maintain high intracellular concentrations of amino acids to match their intracellular osmolarity to the high osmolarity of the environment. These oxidizable amino acids are also used extensively in cellular energy metabolism (Moyes et al, 1990). Therefore, the decreased glycine concentration in the digestive glands of clams under reduced seawater salinities was probably concerned with osmotic regulation, and is consistent with the decreased levels of homarine and hypotaurine.…”

Section: Metabolic Differences In the Digestive Glands Of Clams Exposmentioning

confidence: 60%

The influence of salinity on toxicological effects of arsenic in digestive gland of clam Ruditapes philippinarum using metabolomics

Wu²,

Liu

et al. 2013

Chin. J. Ocean. Limnol.

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Ruditapes philippinarum , a clam that thrives in intertidal zones of various salinities, is a useful biomonitor to marine contaminants. We investigated the infl uence of dilution to 75% and 50% of normal seawater salinity (31.1) on the responses of the digestive gland of R . philippinarum to arsenic exposure (20 μg/L), using nuclear magnetic resonance (NMR)-based metabolomics. After acute arsenic exposure for 48 h, salinity-dependent differential metabolic responses were detected. In normal seawater, arsenic exposure increased the concentrations of branched-chain amino acids, and of threonine, proline, phosphocholine and adenosine, and it decreased the levels of alanine, hypotaurine, glucose, glycogen and ATP in the digestive glands. Differential changes in metabolic biomarkers observed at lower salinity (~23.3) included elevation of succinate, taurine and ATP, and depletion of branched-chain amino acids, threonine and glutamine. Unique effects of arsenic at the lowest salinity (~15.6) included down-regulation of glutamate, succinate and ADP, and up-regulation of phosphocholine. We conclude that salinity infl uences the metabolic responses of this clam to arsenic.

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Section: Metabolic Differences In the Digestive Glands Of Clams Exposmentioning

confidence: 60%

The influence of salinity on toxicological effects of arsenic in digestive gland of clam Ruditapes philippinarum using metabolomics

Wu²,

Liu

et al. 2013

Chin. J. Ocean. Limnol.

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“…However, no significant alteration in osmolytes (e.g., betaine, homarine, taurine, glycine, and hypotaurine) was observed. In other metabolic pathways, the large pools of oxidizable amino acids can be used extensively in cellular energy metabolism [21,22]. Therefore, the elevated amino acids implied the depleted energy demand in V. harveyi-challenged White clam samples, which was consistent with the elevated glucose and glycogen, and depleted ATP and succinate, an intermediate of Krebs cycle.…”

Section: Differential Metabolic Responses In Hepatopancreas From Whitmentioning

confidence: 87%

Metabolomic analysis revealed the differential responses in two pedigrees of clam Ruditapes philippinarum towards Vibrio harveyi challenge

Liu

Zhao

et al. 2013

Fish & Shellfish Immunology

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a b s t r a c tManila clam Ruditapes philippinarum is an important marine aquaculture shellfish. This species has several pedigrees including White, Zebra, Liangdao Red and Marine Red distributing in the coastal areas in North China. In this work, we studied the metabolic differences induced by Vibrio harveyi in hepatopancreas from White and Zebra clams using NMR-based metabolomics. Metabolic responses (e.g., amino acids, glucose, glycogen, ATP and succinate) and altered mRNA expression levels of related genes (ATP synthase, heat shock protein 90, defensin and lysozyme) suggested that V. harveyi induced clear disruption in energy metabolism and immune stresses in both White and Zebra clam hepatopancreas. However, V. harveyi caused obvious osmotic stress in Zebra clam hepatopancreas, which was not observed in V. harveyi-challenged White clams samples. In addition, V. harveyi challenge induced more severe disruption in energy metabolism and immune stress in White clams than in Zebra clams. Overall, our results indicated that the biological differences between different pedigrees of R. philippinarum should be considered in immunity studies.

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“…However, as discussed by Stuart & Ballantyne (1996) and Viant et al (2003), there is some evidence that marine molluscs are less reliant on fatty acid and ketone body metabolism compared with mammals and freshwater molluscs. The physiological basis for this may be that some marine molluscs use high intracellular concentrations of free amino acids to balance their intracellular osmolarity with the environment (Viant et al 2003) and that these pools of oxidisable amino acids are also used extensively in cellular energy metabolism (Moyes et al 1990). This is consistent with results seen in starved disk abalone (Haliotis discus) (Watanabe et al 1993) and in diseased and stunted red abalone H. rufescens (Viant et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Metabolic profiling of Mytilus galloprovincialis and its potential applications for pollution assessment

Jones¹,

Dondero²,

Viarengo³

et al. 2008

Mar. Ecol. Prog. Ser.

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Monitoring of the marine environment is crucial for determining the impact of environmental stressors and/or pollution on ecosystem health. Classical assays that traditionally focus on a limited number of selected endpoints have thus far proven to be of limited success in this regard. Here, we apply 1 H-nuclear magnetic resonance (NMR)-based chemometric analysis to the marine mussel Mytilus galloprovincialis to investigate changes in the metabolic profile of digestive gland tissue as a response to exposure to nickel and chlorpyrifos, both as single chemicals and as a mixture. The multivariate data was analysed by principal component analysis and partial least-squares discriminant analysis. The major metabolite changes responsible for the spectral differences observed were related to amino acid, nucleotide and methylamine metabolism in all 3 cases. In addition, novel metabolic profiles were associated with exposure to each chemical and the mixture. Nickel produced changes in energy metabolism while chlorpyrifos resulted in an increase in acetylcholine levels. Mussels exposed to a single compound demonstrated a significantly different response to those given the mixture, where a small antagonistic effect was evident. The study demonstrates the potential of NMR-based metabolomics to provide a rapid and cost-effective screening tool for monitoring the aquatic environment.KEY WORDS: Antagonism · Metabonomics · Metabolomics · Mussels · Pollution · Nickel · Chlorpyrifos Resale or republication not permitted without written consent of the publisherMar Ecol Prog Ser 369: [169][170][171][172][173][174][175][176][177][178][179] 2008 molecule metabolites to be considered simultaneously. NMR-based metabolomics is becoming increasingly prevalent in environmental toxicology research, particularly in terrestrial studies (Griffin & Shore 2007). The uses and applications of this technique in marine science to date are limited but are summarised in Viant (2007).Mussels are well-suited to metabolomic analysis for environmental toxicology. They have a wide geographical distribution in both salt and freshwaters and, as sessile, filter-feeding organisms, they are exposed to and bio-accumulate many different classes of environmental contaminants. This, in conjunction with their importance as a food organism to humans, means they are widely used in ecological and bio-monitoring studies (Viarengo & Canesi 1991, Cajaraville et al. 2000. Therefore integrating ecotoxicogenomics-based data derived from metabolomics into environmentally relevant results is likely to be much easier for these species than for many others.In the present study, we applied 1 H-NMR-based metabolomic and chemometric analysis to the marine mussel Mytilus galloprovincialis. Changes in the metabolic profile of digestive gland tissue as a response to exposure to the heavy metal nickel (Ni) and the organophosphorus insecticide chlorpyrifos (Chlp), both singly and as a mixture, were assessed. Both compounds occur in the marine environment and their effects ar...

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A comparison of fuel preferences of mitochondria from vertebrates and invertebrates

Cited by 47 publications

References 151 publications

The influence of salinity on toxicological effects of arsenic in digestive gland of clam Ruditapes philippinarum using metabolomics

The influence of salinity on toxicological effects of arsenic in digestive gland of clam Ruditapes philippinarum using metabolomics

Metabolomic analysis revealed the differential responses in two pedigrees of clam Ruditapes philippinarum towards Vibrio harveyi challenge

Metabolic profiling of Mytilus galloprovincialis and its potential applications for pollution assessment

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