A comparison of lymphocyte migration through intestinal lymph nodes, subcutaneous lymph nodes, and chronic inflammatory sites of sheep

Chin, Warren; Hay, John B.

doi:10.1016/0016-5085(80)90918-x

Cited by 92 publications

(20 citation statements)

References 12 publications

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“…Therefore, we addressed whether ovine γδ T cells leaving extralymphoid tissues via afferent lymph display characteristics of professional APCs. By using an ovine model of afferent lymph vessel cannulation (Young et al, 1997), we were able to examine skin-draining lymph from uninflamed and chronically inflamed skin in each animal, which allowed for the analysis and comparison of γδ T cells in the steady state and in the presence of chronic inflammation elicited by Complete Freund’s Adjuvant (Brown et al, 2010; Chin and Hay, 1980). We stained γδ T cells draining skin for molecules that indicate the ability to present antigen and provide costimulatory signals and compared expression levels to that of co-isolated dendritic cells in the draining lymph.…”

Section: Resultsmentioning

confidence: 99%

Ovine skin-recirculating γδ T cells express IFN-γ and IL-17 and exit tissue independently of CCR7

Geherin

Lee

Wilson

et al. 2013

Veterinary Immunology and Immunopathology

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γδ T cells continuously survey extralymphoid tissues, providing key effector functions during infection and inflammation. Despite their importance, the function and the molecules that drive migration of skin-recirculating γδ T cells are poorly described. Here we found that γδ T cells traveling in the skin-draining afferent lymph of sheep are effectors that produce IFN-γ or IL-17 and express high levels of the skin- and inflammation-seeking molecule E-selectin ligand. Consistent with a role for chemokine receptor CCR7 in mediating T cell exit from extralymphoid tissues, conventional CD4 and CD8 T cells in skin-draining lymph were enriched in their expression of CCR7 compared to their skin-residing counterparts. In contrast, co-isolated γδ T cells in skin or lymph lacked expression of CCR7, indicating that they use alternative receptors for egress. Skin-draining γδ T cells were unresponsive to many cutaneous and inflammatory chemokines, including ligands for CCR2, CCR4, CCR5, CCR8, CCR10, and CXCR3, but showed selective chemotaxis toward the cutaneously expressed CCR6 ligand CCL20. Moreover, IL-17+ γδ T cells were the most CCL20-responsive subset of γδ T cells. The data suggest that γδ T cells survey the skin and sites of inflammation and infection, entering via CCR6 and E-selectin ligand and leaving independent of the CCR7-CCL21 axis.

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Section: Resultsmentioning

confidence: 99%

Ovine skin-recirculating γδ T cells express IFN-γ and IL-17 and exit tissue independently of CCR7

Geherin

Lee

Wilson

et al. 2013

Veterinary Immunology and Immunopathology

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“…1); however, there are more tissue lymphocytes in sites of inflammation, and chronic inflammation also dramatically enhances lymphocyte migration from blood into the inflamed site (5). To test whether the efficiency of tissue egress is itself affected by chronic inflammation, or if enhanced exit could be explained solely by increased tissue lymphocyte numbers, we turned to the mouse model.…”

Section: Resultsmentioning

confidence: 99%

“…Classic studies in the sheep showed that large numbers of lymphocytes leave the site of inflammation via the afferent lymph (5, 34). Our experiments extend these early studies by revealing that chronic inflammation dramatically boosts the egress rate of memory/effector (L-selectin lo/- and E-selectin ligand + ) CD4 and CD8 T cells from the affected site (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The cannulated lymphatics drained the skin, muscles and bones of the rear flank. In sheep, inflammation was induced by subcutaneous injection of 0.3 – 0.5 ml CFA emulsified with saline into 2 injection sites either less than 48 hours (acute inflammatory phase) or more than 3 weeks (chronic inflammatory phase) before experimentation as described (5, 31-34). In mice, inflammation was induced by subcutaneous injection of 10μl non-emulsified CFA into the rear footpads using a 10μl Hamilton syringe.…”

Section: Methodsmentioning

confidence: 99%

“…After a period of residency in lymph nodes, naïve and antigen-experienced T cells enter efferent lymph sinuses, which drain into efferent lymph vessels, and via the thoracic duct, back into the blood. On average, lymphocytes require 24h to migrate from blood into and through extralymphoid tissue and back into afferent lymph (5). Parabiotic mouse models also illustrate that memory CD8 T cells rapidly turn over in many extralymphoid tissues (6).…”

Section: Introductionmentioning

confidence: 99%

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Chemoattractant Receptors and Lymphocyte Egress from Extralymphoid Tissue: Changing Requirements during the Course of Inflammation

Brown

Fintushel

Lee

et al. 2010

The Journal of Immunology

Self Cite

121

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Memory/effector T cells traffic efficiently through extralymphoid tissues, entering from the blood and leaving via the afferent lymph. During inflammation, T cell traffic into the affected tissue dramatically increases; however, the dynamics and mechanisms of T cell exit from inflamed tissues are poorly characterized. Here we show, using both a mouse and a sheep model, that large numbers of lymphocytes leave the chronically inflamed skin. Many T cells capable of producing IFN-γ and IL-17 also entered the draining afferent lymph, demonstrating that memory/effector T cells egress from sites of inflammation. Whereas efficient egress from acutely inflamed skin required lymphocyte-expressed CCR7, chronic inflammation promoted significant CCR7-independent exit as well. Lymphocyte exit at late time points of inflammation was sensitive to pertussis toxin but only partially affected by the drug FTY720, implying the contribution of alternative chemoattractant receptors other than S1P1. Our data show that CCR7 is an important receptor for lymphocyte egress from both resting and inflamed extralymphoid tissues, but that alternative exit receptors come into play during chronic inflammation.

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Role of Interferon-? in Cutaneous Trafficking of Lymphocytes With Emphasis on Molecular and Cellular Adhesion Events

Nickoloff¹

1988

Arch Dermatol

142

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A comparison of lymphocyte migration through intestinal lymph nodes, subcutaneous lymph nodes, and chronic inflammatory sites of sheep

Cited by 92 publications

References 12 publications

Ovine skin-recirculating γδ T cells express IFN-γ and IL-17 and exit tissue independently of CCR7

Ovine skin-recirculating γδ T cells express IFN-γ and IL-17 and exit tissue independently of CCR7

Chemoattractant Receptors and Lymphocyte Egress from Extralymphoid Tissue: Changing Requirements during the Course of Inflammation

Role of Interferon-? in Cutaneous Trafficking of Lymphocytes With Emphasis on Molecular and Cellular Adhesion Events

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