A comparison of methods of seed tuber inoculation for assessing the susceptibility of potato cultivars to blackleg (Erwinia carotovora subsp. atroseptica) in the field

Lapwood, D. H.; Read, P. J.

doi:10.1111/j.1744-7348.1986.tb05320.x

Cited by 8 publications

(4 citation statements)

References 14 publications

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“…Most of these plants were actually diseased early on; the diseased parts died and disappeared, and new, symptomless stems emerged, which were often frail and did not lead to any significant tuber production. This observation is consistent with earlier reports (Lapwood & Read, 1986), and probably explains the fact that yield losses in symptomless plants were highest for plants scored at the latest assessment dates. Inoculation method and inoculum concentration were major factors for the subsequent development of symptoms.…”

Section: Discussionsupporting

confidence: 93%

Development of symptoms caused by Erwinia carotovora ssp. atroseptica under field conditions and their effects on the yield of individual potato plants

Hélias¹,

Andrivon²,

Jouan³

2000

Plant Pathology

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Infection of seed tubers by pectinolytic Erwinia species can lead to the development of various symptoms during vegetative growth of potato crops, including non‐emergence of plants, chlorosis, wilting, haulm desiccation and typical blackleg. The relationships between types of symptoms and yield are poorly documented, and are investigated by following the development of symptoms in potato plants grown under field conditions from seed tubers artificially inoculated with E. carotovora ssp. atroseptica (Eca), and measuring the yield of each plant. Symptoms were classified into five main types (non‐emergence, wilting/chlorosis, blackleg, haulm desiccation and plant death). Each plant was scored for types of symptom on four successive dates; plants without visible symptoms were scored as healthy. The method of inoculation and inoculum concentration proved major factors for the subsequent development of symptoms. Disease development was more severe after vacuum infiltration of bacteria into seed tubers than after shaking tubers in contaminated sand. Disease usually progressed from chlorosis and/or wilting to partial or total desiccation on a given plant. Yield losses varied according to symptom type, but the relationship between symptoms recorded and yield also depended on scoring dates. The data suggest that the beginning of tuber growth might be the most suitable stage for predicting yield losses from symptom observations. In both cultivars studied (Bintje, highly susceptible, and Désirée, moderately resistant), the yield of symptomless plants growing from inoculated seed tubers was significantly less than that of control plants, indicating that the presence of bacteria on the seed tuber was detrimental, even in the absence of visible symptoms. Differences in symptom expression in the field between cultivars matched the level of visible infection of tubers at harvest, as Bintje tubers showed a higher incidence of rot than Désirée tubers.

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Section: Discussionsupporting

confidence: 93%

Development of symptoms caused by Erwinia carotovora ssp. atroseptica under field conditions and their effects on the yield of individual potato plants

Hélias¹,

Andrivon²,

Jouan³

2000

Plant Pathology

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“…Methods are only suitable if they are easy to perform, applicable to early vegetative generations and yield reproducible and quantitative data that show good agreement with the resistance of the clones in farmers' fields. Numerous methods for screening of plants in the field [Hossain and Logan, 1983;Lapwood and Legg, 1983;Lapwood and Gans, 1984;Lapwood and Read, 1986a;Gans et al, 1991], or in the glasshouse [Munzert, 1975;Hidalgo and Echandi, 1982;Lapwood and Read, 1986b;Lojkowska and Kelman, 1989] have been described. Field tests correspond most to field conditions but they are expensive to perform and not feasible when only a small amount of seed is available.…”

Section: Introductionmentioning

confidence: 99%

The role of the seed tuber in determining partial resistance to potato blackleg caused byErwinia spp.

Allefs¹,

Dooijeweert

Jong

et al. 1995

Eur J Plant Pathol

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In t991 and 1992, 12 potato cultivars were screened at two locations for resistance to blackleg, after vacuum infiltration of the seed with Erwinia carotovora subsp, atroseptica or E. chrysanthemi. Cultivar differences for resistance to E.c. subsp, atroseptica and E. chrysanthemi were found which were consistent over locations and years. Seed tubers of the same cultivars were also screened for resistance to both Erwinia spp. by using a tuber slice inoculation method. Correlation coefficients for comparisons between resistance to blackleg in the field and tuber tissue resistance under aerobic or anaerobic conditions were not significant. This could partly be explained by drastic changes in relative tuber tissue resistance of the cultivars within a 5 weeks period after planting in the field. Presprouting of seed tubers in diffuse daylight had a less pronounced effect on relative tuber tissue resistance than planting in the field. Monitoring the process of mother tuber decay during the growing season of 1993 after vacuum infiltration with E.c. subsp, atroseptica and E. chrysanthemi revealed that cultivars differed in the extent to which these bacteria enhanced the process of mother tuber decay. These differences partly explained the cultivar differences for resistance to blackleg in the field.Abbreviations: Eca = Erwinia carotovora subsp, atroseptica; Ech = Erwinia chrysanthemi; NOP = Noordoostpolder; Wag --Wageningen

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“…could be utilized to increase resistance in potatoes ( Pérombelon & Salmond, 1995). Under field conditions, cultivars could be classified as resistant, susceptible and intermediate ( Lapwood & Gans, 1984; Lapwood & Read, 1986; Bain & Pérombelon, 1988; Bain et al ., 1990 ).…”

Section: Introductionmentioning

confidence: 99%

Internal colonization pathways of potato plants by Erwinia carotovora ssp. atroseptica

Hélias¹,

Andrivon²,

Jouan³

2000

Plant Pathology

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Transmission of pectinolytic Erwinia species from infected mother tubers to daughter tubers has been studied mainly through detection tests, carried out at harvest, on limited samples of tubers produced by plants grown from artificially inoculated mother tubers. However, detection has not been performed on samples collected at different stages of crop development, in order to follow the contamination progress in different organs through the plants to the progeny tubers. In this study the bacterial contamination of progeny tubers was investigated by detecting Erwinia carotovora ssp. atroseptica in different symptomless plant organs (stolons, stems, progeny tubers) and in the parts with or without symptoms of diseased stems, collected at various stages of crop development. Infection levels in below‐ and above‐ground organs of plants of two cultivars differing in their resistance to Erwinia, infected by either vacuum infiltration or sand wounding, were monitored throughout the growing season and at harvest using DAS‐ELISA and PCR. Detection tests showed that healthy organs from symptomless plants were less frequently contaminated than symptomless organs from diseased plants, and that stolons were precociously and more frequently contaminated than stems and daughter tubers, irrespective of the health of the plant. Stem infections were shown to progress latently in the stem, bacteria usually being recovered 10–15 cm past visible lesions. In many cases, typical aerial stem‐rot symptoms could be related to this upward movement of bacteria from the infected mother tuber. Daughter tubers without symptoms were shown to be frequently contaminated, usually at heel ends, suggesting internal contamination from mother tuber to progeny.

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A comparison of methods of seed tuber inoculation for assessing the susceptibility of potato cultivars to blackleg (Erwinia carotovora subsp. atroseptica) in the field

Cited by 8 publications

References 14 publications

Development of symptoms caused by Erwinia carotovora ssp. atroseptica under field conditions and their effects on the yield of individual potato plants

Development of symptoms caused by Erwinia carotovora ssp. atroseptica under field conditions and their effects on the yield of individual potato plants

The role of the seed tuber in determining partial resistance to potato blackleg caused byErwinia spp.

Internal colonization pathways of potato plants by Erwinia carotovora ssp. atroseptica

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