A Comparison of Phosphomonoesterase Activities in the Seminal Plasmas of the Domestic Cock, Turkey Tom, Boar, Bull, Buck Rabbit and of Man

Bell, Diana; Lake, P. E.

doi:10.1530/jrf.0.0030363

Cited by 31 publications

(23 citation statements)

References 8 publications

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“…Several reports in the literature indicated that the level of phosphatases differs greatly between species (BELL and LAKE, 1962;MANN, 1964;and MURDOCH and WHITE, 1968). O u r finding that buffalo semen had almost 150 O/o and 250 O / o higher alkaline and acid phosphatase activity, respectively, than that of Friesian and native bulls agrees with the findings of ROY et al (1960).…”

Section: Discussionsupporting

confidence: 84%

Comparative Study of the Phosphatase Activity in the Semen of Bovines (Bos bubalis and Bos taurus) in Egypt

Abdou

El-Guindi

Mostafa

et al. 2010

Zentralblatt Für Veterinärmedizin Reihe A

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Summary The paper records the average values of acid and alkaline phosphatases in 102 (first, second and third) ejaculates from buffalo, Friesian, and native bulls maintained at 3 different localities. The levels of these enzymes were studied in both whole semen and seminal plasma. The results were examined to reveal the difference between buffalo and cattle, and between consecutive ejaculates, and also the variation in phosphomonoesterase activity of semen in relation to the locality. Zusammenfassung Vergleichende Untersuchungen über die Phosphatase‐Aktivität im Sperma von Boviden (Bos bubalis und Bos taurus) in Ägypten Die Aktivität der sauren und alkalischen Phosphatase in drei aufeinanderfolgenden Ejakulaten von 15 Büffeln, 6 Friesen‐Bullen und 2 im Lande gezüchteten Baladi‐Bullen wurde untersucht. Die Enzymwerte wurden sowohl im Vollsperma als auch im Spermaplasma bestimmt. Die Ergebnisse wurden einerseits hinsichtlich der Differenzen zwischen den Büffeln und Rindern und anderseits zwischen den aufeinanderfolgenden Ejakulaten ausgewertet. Im weiteren wurden die Unterschiede der Phosphomonoesterase‐Aktivität des Samens hinsichtlich des verschiedenen Standortes der Probanden geprüft. Résumé Etude comparative de l'activité des phosphatases dans le sperme de bovins (Bos bubalis et Bos taurus) en Egypte On a examiné l'activité des phosphatases acides et alcalines dans trois éjaculats successifs de 15 buffles, 6 taureaux frisons et 2 taureaux Baladi élevés dans le pays. On a déterminé les valeurs enzymatiques aussi bien dans le sperme complet que dans le plasma du sperme. On a donné les résultats d'une part en fonction des différences entre les éjaculats successifs. On a testé de plus les différences de l'activité de la phosphomonoesterase du sperme en rapport avec les différents lieux de prélèvement. Resumen Estudio comparativo de la actividad fosfatásica en el semen de bovinos (Bos bubalis y Bos taurus) en Egipto Se analizó la actividad de las fosfatasas ácida y alcalina en tres eyaculados consecutivos de 15 búfalos, 6 toros de la raza frisia y 2 toros baladi autóctonos en 3 localidades. Los valores enzimáticos se valoraron en el esperma íntegro y en el plasma seminal. Los resultados se examinaron para revelar por un lado las diferencias entre los búfalos y los toros, y por otro entre los eyaculados consecutivos. Además se examinaron las variaciones en la actividad de la fosfomonoestearasa del semen en relación con los lugares diferentes en que se hallaban los animales en cuestión.

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Section: Discussionsupporting

confidence: 84%

Comparative Study of the Phosphatase Activity in the Semen of Bovines (Bos bubalis and Bos taurus) in Egypt

Abdou

El-Guindi

Mostafa

et al. 2010

Zentralblatt Für Veterinärmedizin Reihe A

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“…Material was obtained for staining from the caput flexure of the epididymis and the cauda epididymidis of four rabbits, six hamsters and six rats immediately after death, and ejaculated spermatozoa were collected from two rabbits by means of an artificial vagina (Walton, 1958 Every sample was washed three times in normal saline, centrifuging each time at low speed for about 2 min, in order to remove excess epididymal plasma which might itself contain alkaline phosphatase (Bell & Lake, 1962). The procedure was shown to be absolutely necessary in the golden hamster where the content of alkaline phosphatase in epididymal plasma was so high that, after staining, the spermatozoa were completely obscured on the slides as a result of a generalized reaction.…”

Section: Methodsmentioning

confidence: 99%

Alkaline Phosphatase in the Cytoplasmic Droplet of Mammalian Spermatozoa

Moniem¹,

Glover²

1972

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“…The activity of AP in the seminal plasma of the fowl is extremely high, and, when compared with that of several mammals, only human seminal plasma shows a higher activity (Bell & Lake, 1962). Since the greater part of the AP of fowl semen was present mainly in small particles in seminal plasma, and since it was difficult to be absolutely certain that washed spermatozoa were free from contamination with the particles, the amount of AP activity in the spermatozoa remains doubtful.…”

Section: Assay Of Enzymesmentioning

confidence: 97%

The Distribution of Some Hydrolytic Enzymes in the Semen of the Domestic Fowl, Gallus Domesticus

McIndoe¹,

Lake²

1974

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Summary. The distribution of some hydrolytic enzymes in fowl semen was studied. Washed spermatozoa and seminal plasma were examined for the following enzymes: acid phosphatase, sulphatase, N-acetyl-\g=b\-dglucosaminidase, \g=b\-glucuronidase,phospholipase A, trypsin-like enzyme and neuraminidase. Only a trypsin-like enzyme, N-acetyl-\g=b\-d-glucosaminidase and acid phosphatase were detected in the semen. Most of the acid phosphatase activity occurred in particulate material in the seminal plasma, whereas virtually all the trypsin-like activity was in the spermatozoa. The N-acetyl-\g=b\-d-glucosaminidaseactivity in semen occurred both in the spermatozoa and the seminal plasma; the proportion of the activity in whole semen contributed by the spermatozoa was less than that contributed by the seminal plasma but the concentration of activity was greater in the spermatozoa.A slightly inhibitory action of seminal plasma on the activity of the trypsin-like enzyme was indicated.A preliminary examination was made of the semen of different strains of fowl and revealed between-strain differences in the activity of enzymes, particularly acid phosphatase.

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A Comparison of Phosphomonoesterase Activities in the Seminal Plasmas of the Domestic Cock, Turkey Tom, Boar, Bull, Buck Rabbit and of Man

Cited by 31 publications

References 8 publications

Comparative Study of the Phosphatase Activity in the Semen of Bovines (Bos bubalis and Bos taurus) in Egypt

Comparative Study of the Phosphatase Activity in the Semen of Bovines (Bos bubalis and Bos taurus) in Egypt

Alkaline Phosphatase in the Cytoplasmic Droplet of Mammalian Spermatozoa

The Distribution of Some Hydrolytic Enzymes in the Semen of the Domestic Fowl, Gallus Domesticus

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