A cryopreservation protocol for immature zygotic embryos of species of Ilex (Aquifoliaceae)

Mroginski, L. A.; Sansberro, Pedro Alfonso; Scocchi, Adriana; Luna, Claudia Verónica; Rey, H. Y.

doi:10.32604/biocell.2008.32.033

Cited by 14 publications

(3 citation statements)

References 9 publications

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“…Therefore, for the present investigation, the following hypotheses were raised: (1) In different accessions of vanilla acclimatized in a greenhouse and subsequently multiplied in vitro, the presence of fungal endophytes will be low or non-existent due to the conditions provided in a balanced environment, guaranteeing the availability of healthy plant material for fulfilment of Koch's third postulate [25,26]. (2) Fusarium species obtained from symptomatic tissue and inoculated into healthy vanilla accessions multiplied in vitro will all induce symptoms in the hosts tested.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Evaluation of the Development of Fusarium in Vanilla Accessions

et al. 2022

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Vanilla is an economically important crop for low-lying humid tropical regions. World demand for natural vanilla is increasing, but cultivated plants face serious phytosanitary problems. The disease known as Fusarium wilt is mainly related to the fungus Fusarium oxysporum f. sp. vanillae, and for its management, the pathogen–host relationship must be understood. Four in vitro multiplied vanilla accessions were evaluated: two Vanilla planifolia from Colombia and Mexico, one from V. odorata, and one (1) F1 hybrid (V. rivasii × V. trigonocarpa). In addition, three isolates of Fusarium from different symptomatic plants present in small-scale agroforestry systems: (1Fov) F. oxysporum f. sp. vanillae from leaf, (2Fov) F. oxysporum f. sp. vanillae from root and (3Fs) F. solani also from root. Plants with two months of growth were inoculated in vitro by immersion of roots, and the development of Fusarium wilt was recorded for 15 days, using a severity scale to describe symptoms and to calculate the area under the disease progress curve (AUDPC). No statistical differences were found when analyzing the interaction between Fusarium isolates and vanilla accessions. However, when independently analyzing the design factor Fusarium isolates, there were significant differences; the 1Fov isolate of F. oxysporum f. sp. vanillae induced the highest symptoms as well as death in some plants of all accessions, while F. solani was considered a secondary pathogen. There were no statistical differences for the vanilla accessions factor, but the values of AUDPC and symptoms observed suggest a slight resistance in all the accessions. Therefore, it is suggested to explore the vanilla gene pool to generate multiplication material with resistance genes and to contribute with genetic improvement to successfully integrate the management of Fusarium wilt in commercial systems.

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Section: Introductionmentioning

confidence: 99%

In Vitro Evaluation of the Development of Fusarium in Vanilla Accessions

et al. 2022

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“…cell suspensions and calli (Withers and Engelmann, 1997)), and even differentiated structures such as the shoot apices of cold-tolerant species (e.g. Reed and Chang, 1997) and in isolated cases, excised embryonic axes/zygotic embryos from recalcitrant seeds (Mroginski et al, 2008).…”

Section: Routine Operations: Recalcitrant Seeds and Vegetatively Prop...mentioning

confidence: 99%

New technologies to improve the ex situ conservation of plant genetic resources

Hay¹

2021

Burleigh Dodds Series in Agricultural Science

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Access to plant genetic resources is fundamental to the development of more resilient and nutritious crops. The efficient and effective conservation of plant genetic resources is therefore key to ensuring global food security. There are more than 1750 genebanks around the world, storing various types of plant germplasm including tissue cultures, seeds, embryos and pollen. This chapter describes some of the recent applications of science and technology to improve the management of genebank collections of orthodox seeds, not least through the introduction of automation. In addition, the chapter looks at how routine cryopreservation procedures are now possible for many species, and how storage procedures for recalcitrant-seeded and vegetatively propagated species continue to evolve given the challenges they present in terms of long term ex situ germplasm conservation.

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“…I. tarapotina and I. vomitoria are used for making stimulatory beverages. Other species of Ilex ( I. cornuta, I. aquifolium, I. crenata and I. opaca ), commonly called “ hollies ”, are usually cultivated for Christmas tree production or landscaping [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Phytochemical and Biological Screening of Leaf, Bark and Fruit Extracts from Ilex dipyrena Wall.

Ali

Khuda

Nazir

et al. 2021

Life

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The Aquifoliaceae is an important family and has been used traditionally for some time. One of the members of this family is the Ilex dipyrena wall, which itself possesses a potential medicinal importance. This plant is traditionally used for the treatment of various ailments including pain, swelling, burns, and fever. The current study was designed to screen out the antioxidant and analgesic potential of this plant and to verify its traditional uses, along with its phytochemical profile. Extracts were subjected to antioxidant, analgesic, and phytochemical analysis using DPPH, chemical-induced (acetic acid and formalin) nociception models and GC-MS analysis, respectively. The leaf, bark, and fruit extracts showed significant antioxidant activity compared to that of standard. Likewise, all the extracts demonstrated significant (p < 0.01) analgesic activity in a mice model. In acetic acid induced analgesia, the leaf, bark, and fruit extracts caused 51.64, 56.13 and 59.52% inhibition, respectively at a dose of 100 mg/kg while at 200 mg/kg it showed 83.01, 71.69 and 75.47% inhibition, respectively. In Formalin-induced paw-licking assay, fruit extract showed 59.42 and 64.19% inhibition at 200 mg/kg dose in the first and second phase, respectively. The GC-MS analysis revealed the presence of cathinone, phenylpropanolamine, dl-phenylephrine, amphetamine, myristic acid, and palmitic acid. Results of the study suggest that crude extracts from different parts of this plant may be a useful source for the development of novel analgesics. However, further investigation in terms of isolation of bioactive compounds and their toxicological evaluations are needed to validate the observed results.

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A cryopreservation protocol for immature zygotic embryos of species of Ilex (Aquifoliaceae)

Cited by 14 publications

References 9 publications

In Vitro Evaluation of the Development of Fusarium in Vanilla Accessions

In Vitro Evaluation of the Development of Fusarium in Vanilla Accessions

New technologies to improve the ex situ conservation of plant genetic resources

Phytochemical and Biological Screening of Leaf, Bark and Fruit Extracts from Ilex dipyrena Wall.

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