A Determinative Scheme for the Identification of Certain Genera of Gram‐negative Bacteria, With Special Reference to the Pseudomonadaceae

Bacterial Populations in Water Masses of different Organic Matter Concentrations in Lake Biwa

“…Isolation of bacteria was made by the spread method. Taxonomical studies were made according to the scheme of SHEWAN et al (1960) modified by SIMIZU (1973) with 392 strains isolated from colonies grown on the agar plates containing 0.5 and 5.0 g peptone per 1, 000 ml. The ability to digest chitin of the isolated strain was examined by observing the formation of a clear zone around the colony grown on the agar plate containing chitin powder.…”

A Comparison of Two Methods for Detecting Attack on Glucose by Pseudomonads and Achromobacters

“…Stanier, Palleroni & Doudoroff (1966) found that P. maltophilia utilized glucose as carbon +energy source when methionine, required as a growth factor, was present. Representatives of Pseudomonas group I11 and group IV of Shewan et al (1960), which by definition do not produce acid in O/F glucose media, were found to attack glucose when tested by the enzyme method (Table 1).…”

Effect of Lactobacillus rhamnosus cells against specific and native fish spoilage bacteria and their spoilage indices on Asian seabass fish chunks

“…Bacteria that are able to produce hydrogen sulphide from either sulphur source appeared as black colonies due to precipitation of ferrous sulphide in the agar. Non-hydrogen sulphide producing bacteria appeared as white colonies (Shewan et al, 1960). The isolates of S. putrefaciens and P. fluorescens were maintained in BHI broth (Hi-°M edia, India) containing 20% glycerol at -80 C. Successive specific spoilage bacterial cultures were inoculated into BHI broth and revitalized before the experiments.…”

“…Confirmation of specific spoilage bacteria : S.putrefaciens and P. fluorescens were characterized using tests such as Gramreaction (Gram et al, 1987), production of DNAase, decarboxlyase and ornithine (Shewan et al, 1960). Specific spoilage bacteria such as S. putrefaciens ATCC 8071 and P. fluorescens ATCC 1352 were procured from the American Culture Type Collection (ATCC) and their cultural characteristics were compared with the specific spoilage bacterial isolates (Stenstrom and Molin, 1990) used in this study.…”