A distinct tospovirus causing necrotic streak on Alstroemeria sp. in Colombia

Abstract

A tospovirus causing necrotic streaks on leaves was isolated from Alstroemeria sp. in Colombia. Infected samples reacted positively with tomato spotted wilt virus (TSWV) antiserum during preliminary serological tests. Further analysis revealed a close serological relationship to tomato chlorotic spot virus (TCSV) and groundnut ringspot virus (GRSV). A major part of the S-RNA segment, encompassing the nucleocapsid (N) protein gene, the 5′ untranslated region and a part of the intergenic region 3′ of the N gene, was cloned and sequenced. The deduced N protein sequence showed highest amino acid identity (82%) to that of TCSV, indicating that the virus represents a new tospovirus species, for which the name Alstroemeria necrotic streak virus (ANSV) is coined. Phylogenetic analysis based on the N protein sequence revealed that this Alstroemeria-infecting tospovirus clustered with tospoviruses from the American continent. Frankliniella occidentalis was identified as potential vector species for ANSV.

A new tospovirus species infectingSolanum esculentumandCapsicum annuumin Thailand

“…Recent advances in molecular techniques have now enabled the discovery of at least 19 distinct tospovirus species with one or more tospovirus species now found on every continent except Antarctica (Pappu et al 2009). Along with TNRV, the number of tospovirus species described is likely to continue to increase with at least two further species being recently proposed (Ciuffo et al 2009;Hassani-Mehraban et al 2010).…”

Evaluation of Seed Transmissibility of Soybean Vein Necrosis-Associated Virus in Two Soybean Cultivars Grown Under Field Conditions

“…However, transmission of SVNaV mechanically from soybean to soybean or from experimental hosts to soybean has been difficult and inefficient [6,8,9]. It should be noted that SVNaV is not an exception in this regard as difficulties in mechanical inoculation of Alstroemeria necrotic streak virus, another tospovirus, to its respective natural host have also been reported [13]. The nucleotide sequence data corresponding to complete nucleocapsid protein gene of 13 isolates reported in this article have been submitted to GenBank and assigned the accession numbers KJ955706 to KJ955718.…”

Dna Barcoding and Development of Species-Specific Markers for the Identification of Tea Mosquito Bugs (Miridae: Heteroptera) in India

“…Among the known 6,000 species of Thrips (Thripidae) (Mound & Morris 2007) nearly 1% are pests of agricultural crops and the following 14 species are reported as vectors of Tospovirus: Thrips tabaci Lindeman, T. palmi Karny, T. setosus Moulton, Scirtothrips dorsalis Hood, Frankliniella occidentalis (Pergande), F. schultzei (Trybom), F. bispinosa (Morgan), F. cephalica (Crawford), F. fusca (Hinds), F. gemina Bagnall, F. intonsa (Trybom), F. zucchini Nakahara Monteiro, Ceratothripoides claratris (Shumsher) and Dictyothrips betae Uzel (Ullman et al 1997;Mound 2005;Jones 2005;Pappu et al 2009;Ciuffo et al 2010;Hassani-Mehraban et al 2010). Most of them are highly polyphagous, have overlapping host ranges, thus being collected together which makes their identification difficult, even though morphological identification keys and web-based identification systems are available.…”