A new tospovirus species infectingSolanum esculentumandCapsicum annuumin Thailand

Abstract

Abstract. Based on distinct sequences, serology and host range, isolates of a new tospovirus species infecting field crops of tomato and pepper in Thailand are shown to be members of the provisionally named Tomato necrotic ringspot virus.

Tomato necrotic ring virus (TNRV), a recently described tospovirus species infecting tomato and pepper in Thailand

“…Although preliminary, these observations coupled with the reports of Chiemsombat et al (2010); Seepiban et al (2011) suggest that TNRV is widespread in Thailand. The availability of a TNRV-specific polyclonal antiserum now allows undertaking of more extensive surveys to obtain a better picture of its geographical distribution and impact.…”

“…The virus showed high homology to a recently characterized new tospovirus species, for which the name Tomato necrotic ringspot virus (TNRV) has been suggested (Chiemsombat et al 2010;Seepiban et al 2011).…”

“…FJ946835). T1-isolate showed 99% aa sequence identity to that of TNRV N gene sequence that was deposited in Genbank and is now published (Chiemsombat et al 2010;Seepiban et al 2011). Since both T1-and P1-isolates were almost completely identical, only the current T1 isolate was used for sequence alignments to other reported Asian tospoviruses and TNRV N gene sequences.…”

Inheritance of Tomato Necrotic Ring Virus Resistance in Capsicum annuum

“…Capsicum annuum is a very popular crop in Thailand, of which the production has been declining lately due to tospoviruses in both open field and greenhouse production. Plants are highly susceptible to these viruses at all stages of development, showing severe symptoms, usually necrotic ringspots, on leaves stem and fruits (Chiemsombat et al, 2010). Most of the seeds of blocky sweet pepper varieties grown in Thailand are imported from European seed companies and these varieties are very susceptible to tospoviruses.…”

“…The species were devided into three clades reflecting the original of distribution, American, Eurasian and Asian (Pappu et al, 2009). Furthermore, based on the viral protein sequences, the TNRV isolates cluster within the Asian clade (Chiemsombat et al, 2010;Hassani-Mehraban et al, 2011;Seepiban et al, 2011). Also, TNRV was put in the Asian Tospovirus group together with Capsicum chlorosis virus (CaCV), Watermelon silver mottle virus (WSMoV), Groundnut bud necrosis virus (GBNV), Watermelon bud necrosis virus (WBNV), Calla lily chlorotic spot virus (CCSV), Tomato zonate spot virus (TZSV) and Melon yellow spot virus (MYSV).…”