A dynamic multiple reaction monitoring strategy to develop and optimize targeted metabolomics methods: Analyzing bile acids in capecitabine-induced diarrhea

Wang, Zhipeng; Li, Hanglin; Yun, Yunlei; Wang, Hongsen; Meng, Bosu; Mu, Yuhui; Gao, Shouhong; Xia, Tao; Chen, Wansheng

doi:10.1016/j.jpba.2022.114938

Cited by 6 publications

(3 citation statements)

References 32 publications

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“…The dynamic multiple reaction monitoring (dMRM) data acquisition mode can yield a higher response, symmetrical peak shape because the mass spectrometer scans only certain channels within a specified time window so that more scans can be obtained [ 33 , 34 ]. In this study, the MRM mode and dMRM mode were compared.…”

Section: Resultsmentioning

confidence: 99%

“…However, ACN was chosen as the preferred solvent because it extracted fewer coextracted matrix components [22]. Meanwhile, it was also the major com- The dynamic multiple reaction monitoring (dMRM) data acquisition mode can yield a higher response, symmetrical peak shape because the mass spectrometer scans only certain channels within a specified time window so that more scans can be obtained [33,34]. In this study, the MRM mode and dMRM mode were compared.…”

Section: Extracting Proceduresmentioning

confidence: 99%

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Highly-Selective Analytical Strategy for 90 Pesticides and Metabolites Residues in Fish and Shrimp Samples

et al. 2023

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The analysis of pesticide residues in aquatic products is challenging due to low residue levels and the complex matrix interference. In this study, we developed a simple, fast method for the trace analysis of 90 pesticides and metabolites in aquatic products. The analytes covered a wide polarity range with log Kow (log octanol-water partition coefficient) ranging from −1.2 to 6.37. Grass carp (Ctenopharyngodon idellus) and prawn (Penaeus chinensis) samples were chosen to validate the quantification method. The samples were extracted by 0.2% formic-acetonitrile, cleaned by solid-phase extraction (PRiME HLB), and analyzed by high performance liquid chromatography−tandem mass spectrometry. The results showed good linearities for the analytes and were observed in the range of 0.05–50 μg/L. The recoveries of the method were within 50.4–118.6%, with the relative standard deviations being lower than 20%. The limits of quantifications (LOQs) of the method were in the range of 0.05–5.0 μg/kg, which were superior to values compared with other research. The developed method was applied to detect pesticide residues in prawn samples from eastern coastal areas of China. Three herbicide residues of diuron, prometryn, and atrazine were detected in prawn samples. The method was sensitive and efficient, which is of significance in expanding the screening scope and improving the quantitative analysis efficiency in aquatic products.

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Section: Resultsmentioning

confidence: 99%

Section: Extracting Proceduresmentioning

confidence: 99%

Highly-Selective Analytical Strategy for 90 Pesticides and Metabolites Residues in Fish and Shrimp Samples

et al. 2023

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“…The methodology validation was completed according to FDA guidelines and Chinese Pharmacopoeia (2015 edition), and the linearity, inter- and intra-precision and accuracy, matrix effect and extraction recovery, stability and specificity of this method were validated according to previous study ( Lu et al, 2022 ; Wang et al, 2022 ; Shu et al, 2016 ).…”

Section: Methodsmentioning

confidence: 99%

Pharmacokinetic assessment of tacrolimus in combination with deoxyschizandrin in rats

Sun,

Wang,

Liu

et al. 2024

Front. Pharmacol.

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BackgroundTacrolimus (Tac) is commonly used for postoperative immunosuppressive therapy in transplant patients. However, problems, for example, low bioavailability and unstable plasma concentration, persist for a long time, Studies have reported that the deoxyschizandrin could effectively improve these problems, but the pharmacokinetic parameters (PKs) of Tac combined with deoxyschizandrin are still unknown.MethodIn this study, an UHPLC-MS/MS method has been established for simultaneous quantitation of Tac and deoxyschizandrin. The PKs of Tac influenced by different doses of deoxyschizandrin after single and multiple administrations were analyzed, and the different impact of deoxyschizandrin and Wuzhi capsule on PKs of Tac were compared.ResultThe modified UHPLC-MS/MS method could rapid quantification of Tac and deoxyschizandrin within 2 min using bifendatatum as the internal standard (IS). All items were successfully validated. The Cmax of deoxyschizandrin increased from 148.27 ± 23.20 to 229.13 ± 54.77 ng/mL in rats after multiple administrations for 12 days. After co-administration of 150 mg/mL deoxyschizandrin, Tac had an earlier Tmax and greater Cmax and AUC0–t, and the Cmax and AUC0–t of Tac increased from 14.26 ± 4.73 to 54.48 ± 14.37 ng/mL and from 95.10 ± 32.61 to 315.23 ± 92.22 h/ng/mL, respectively; this relationship was positively proportional to the dosage of deoxyschizandrin. In addition, compared with Wuzhi capsule, the same dose of deoxyschizandrin has a better effective on Tac along with more stable overall PKs.ConclusionAn UHPLC-MS/MS method was established and validated for simultaneous detection of deoxyschizandrin and Tac. Deoxyschizandrin could improve the in vivo exposure level and stability of Tac, besides, this effect is better than Wuzhi capsule in same dose.

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Simultaneous determination of UDCA and its major metabolites in human plasma with surrogate matrix by a rapid and specific LC-MS/MS method

Zhang

et al. 2023

Journal of Chromatography B

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A dynamic multiple reaction monitoring strategy to develop and optimize targeted metabolomics methods: Analyzing bile acids in capecitabine-induced diarrhea

Cited by 6 publications

References 32 publications

Highly-Selective Analytical Strategy for 90 Pesticides and Metabolites Residues in Fish and Shrimp Samples

Highly-Selective Analytical Strategy for 90 Pesticides and Metabolites Residues in Fish and Shrimp Samples

Pharmacokinetic assessment of tacrolimus in combination with deoxyschizandrin in rats

Simultaneous determination of UDCA and its major metabolites in human plasma with surrogate matrix by a rapid and specific LC-MS/MS method

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