A facile approach toward protein-resistant biointerfaces based on photodefinable poly-p-xylylene coating

Su, Chiao-Tzu; Yuan, Ruei-Hung; Chen, Yung-Chih; Lin, Tyrone T.; Chien, Hsiu‐Wen; Hsieh, Chia Chien; Tsai, Wei‐Bor; Chang, Chien Hsin; Chen, Hsien‐Yeh

doi:10.1016/j.colsurfb.2013.11.044

Cited by 13 publications

(11 citation statements)

References 38 publications

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“…PEG-modified surface was previously shown to be protein resistant 36,37 and preventing nonspecific protein adsorption, 38,39 and was used as a control surface where low protein adsorption was expected. 40 Low fibronectin (1.5 ± 0.5 ng cm −2 ) and BMP-2 (3.4 ± 1.1 ng cm −2 ) binding was observed on these reference surfaces, which confirming the successful adsorption of these proteins onto the parylene-C surfaces. QCM dynamic analysis was further used to characterize the adsorption stability for surfaces on which fibronectin or BMP-2 layers were already adsorbed.…”

Section: Resultsmentioning

confidence: 52%

Enhanced bone morphogenic property of parylene-C

et al. 2016

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The ability to induce osteointegration was introduced to a parylene-C surface via the simple and intuitive process of protein adsorption mediated by hydrophobic interactions. In this way, bone morphogenetic protein (BMP)-2, fibronectin, and platelet-rich plasma (PRP) could be immobilized on parylene-C surfaces. This approach alleviates concerns related to the use of potentially harmful substances in parylene-C modification processes. The adsorbed protein molecules were quantitatively characterized with respect to adsorption efficacy and binding affinity, and the important biological activities of the proteins were also examined using both early and late markers of osteogenetic activity, including alkaline phosphatase expression, calcium mineralization and marker gene expression. Additionally, the adsorbed PRP exhibited potential as a substitute for expensive recombinant growth factors by effectively inducing comparable osteogenetic activity. In addition to the excellent biocompatibility of parylene-C and its ability to coat a wide variety of substrate materials, the modification of parylene-C via protein adsorption provides unlimited possibilities for installing specific biological functions, expanding the potential applications of this material to include various biointerface platforms.

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Section: Resultsmentioning

confidence: 52%

Enhanced bone morphogenic property of parylene-C

et al. 2016

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“…Aer the photoimmobilization process conjugated CHX, a weakened 1667 cm À1 band was detected in the spectra (Fig. 2a), and strong absorption from the C-C-OH group was identied at 1711 cm À1 , indicating the excited triplet state [41][42][43] of benzoyl groups. In addition, peaks at 1568 cm À1 and 1510 cm À1 , indicative of a C]N group, 44 peaks at 1035 cm À1 and 931 cm À1 , indicative of a chlorophenyl group, and a band from 3161 cm À1 to 3453 cm À1 , indicative of a -N-H group, were found for the immobilized CHX molecules.…”

Section: Surface Modications and Characterizationmentioning

confidence: 98%

Compatibility balanced antibacterial modification based on vapor-deposited parylene coatings for biomaterials

et al. 2014

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Advanced antibacterial surfaces are designed based on covalently attached antibacterial agents, avoiding potential side effects associated with overdosed or eluted agents. The technique is widely applicable regardless of the underlying substrate material. In addition, antibacterial surfaces are effective against the early stages of bacterial adhesion and can significantly reduce the formation of biofilm, without compromising biocompatibility. Here, this concept was realized by employing a benzoyl-functionalized parylene coating. The antibacterial agent chlorhexidine was used as a proof of concept. Chlorhexidine was immobilized by reaction with photoactivated benzoyl-functionalized surfaces, including titanium alloy, stainless steel, polyether ether ketone, polymethyl methacrylate, and polystyrene. A low concentration of chlorhexidine (1.4 AE 0.08 nmol cm À2 ) covalently bound to surfaces rendered them sufficiently resistant to an Enterobacter cloacae inoculum and its adherent biofilm. Compared to unmodified surfaces, up to a 30-fold reduction in bacterial attachment was achieved with this coating technology. The immobilization of chlorhexidine was verified with infrared reflection absorption spectroscopy (IRRAS) and X-ray photoelectron spectroscopy (XPS), and a leaching test was performed to confirm that the chlorhexidine molecules were not dislodged. Cell compatibility was examined by culturing fibroblasts and osteoblasts on the modified surfaces, revealing greater than 93% cell viability.This coating technology may be broadly applicable for a wide range of other antibacterial agents and allow the design of new biomaterials.

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“…The resultant thickness of the coating was approximately 2000 Å with a uniformity of ±70 Å. The modification of benzoyl-PPX coating provides a reactivity analogous to the benzophenone moiety that can rapidly conjugate with molecules via benzophenone triplet insertion into C-H or N-H bonds under UV irradiation 43,44 . However, the maleimide-PPX modification provides the accessibility to Michael-type nucleophilic addition between the maleimide group and a target molecule that contains a thiol group 45 .…”

Section: Resultsmentioning

confidence: 99%

Characterization of Mechanical Stability and Immunological Compatibility for Functionalized Modification Interfaces

Hsu

Guan

et al. 2019

Sci Rep

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Surface modification layers are performed on the surfaces of biomaterials and have exhibited promise for decoupling original surface properties from bulk materials and enabling customized and advanced functional properties. The physical stability and the biological compatibility of these modified layers are equally important to ensure minimized delamination, debris, leaching of molecules, and other problems that are related to the failure of the modification layers and thus can provide a long-term success for the uses of these modified layers. A proven surface modification tool of the functionalized poly-para-xylylene (PPX) system was used as an example, and in addition to the demonstration of their chemical conjugation capabilities and the functional properties that have been well-documented, in the present report, we additionally devised the characterization protocols to examine stability properties, including thermostability and adhesive strength, as well as the biocompatibility, including cell viability and the immunological responses, for the modified PPX layers. The results suggested a durable coating stability for PPXs and firmly attached biomolecules under these stability and compatibility tests. The durable and stable modification layers accompanied by the native properties of the PPXs showed high cell viability against fibroblast cells and macrophages (MΦs), and the resulting immunological activities created by the MΦs exhibited excellent compatibility with non-activated immunological responses and no indication of inflammation.

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A facile approach toward protein-resistant biointerfaces based on photodefinable poly-p-xylylene coating

Cited by 13 publications

References 38 publications

Enhanced bone morphogenic property of parylene-C

Enhanced bone morphogenic property of parylene-C

Compatibility balanced antibacterial modification based on vapor-deposited parylene coatings for biomaterials

Characterization of Mechanical Stability and Immunological Compatibility for Functionalized Modification Interfaces

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