A fast, sensitive, single-step colorimetric dipstick assay for quantifying ascorbic acid in urine

LeVatte, Marcia; Lipfert, Matthias; Wishart, David S.

doi:10.1016/j.ab.2019.05.015

Cited by 12 publications

(7 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Table 3 lists reported studies for ascorbic acid analysis using PADs with various colorimetric detection methods. 35–37 The analytical features of the cited work are listed for comparison with this study. This work uses a simple chemical reaction, comprising excess KIO 3 and acetic acid to generate volatile iodine, which is proportional to the ascorbic acid content in the sample solution.…”

Section: Resultsmentioning

confidence: 99%

“…, spot test, 35 immersion into a sample solution, 36 and dipstick assay. 37 These methods also did not separate iodine before detection, and thus can be affected by the matrix of the sample. The linear range of this work is 0.284–2.84 mM ascorbic acid, with a LOD of 0.16 mM and precision < 2% RSD, which is fit-for-purpose for the analysis of ascorbic acid in fruit juice samples.…”

Section: Resultsmentioning

confidence: 99%

“…29 Various instrumental methods have been employed for ascorbic acid determination, such as high-performance liquid chromatography (HPLC), 30 electrochemical analysis, 31 capillary electrophoresis, 32 fluorescence, 33 and chemiluminescence. 34 Recently, various paper-based analytical devices (PADs) with simple colorimetric detection involving a spot test, 35 immersion in sample solution, 36 and dipstick assay 37 have been applied for ascorbic acid analysis.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A ninety-six well plate as headspaces with moist starch indicator paper as a cover for the determination of ascorbic acid by iodate oxidation and formation of volatile iodine

et al. 2022

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A ninety-six well plate as headspaces with moist starch indicator paper as a cover for the determination of ascorbic acid by iodate oxidation and formation of volatile iodine

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Colorimetric kits: They enable the detection of L-ascorbic acid in a wide range of biological fluids, tissues, and cells. They are available in 96-well or dipstick formats [250]. The determination is based on the chelation of ferrous iron with a colorimetric probe to produce a compound exhibiting a strong absorbance.…”

Section: Determination Of Vitamin C In Biological Materialsmentioning

confidence: 99%

Vitamin C—Sources, Physiological Role, Kinetics, Deficiency, Use, Toxicity, and Determination

et al. 2021

View full text Add to dashboard Cite

Vitamin C (L-ascorbic acid) has been known as an antioxidant for most people. However, its physiological role is much larger and encompasses very different processes ranging from facilitation of iron absorption through involvement in hormones and carnitine synthesis for important roles in epigenetic processes. Contrarily, high doses act as a pro-oxidant than an anti-oxidant. This may also be the reason why plasma levels are meticulously regulated on the level of absorption and excretion in the kidney. Interestingly, most cells contain vitamin C in millimolar concentrations, which is much higher than its plasma concentrations, and compared to other vitamins. The role of vitamin C is well demonstrated by miscellaneous symptoms of its absence—scurvy. The only clinically well-documented indication for vitamin C is scurvy. The effects of vitamin C administration on cancer, cardiovascular diseases, and infections are rather minor or even debatable in the general population. Vitamin C is relatively safe, but caution should be given to the administration of high doses, which can cause overt side effects in some susceptible patients (e.g., oxalate renal stones). Lastly, analytical methods for its determination with advantages and pitfalls are also discussed in this review.

show abstract

“…Ascorbic acid in urine is a useful indicator of vitamin C intake amount. This water-soluble vitamin and good antioxidant is required for enzymatic activities for norepinephrine biosynthesis and hormone stabilization . Urinary glucose can be found when blood glucose is raised for patients suffering from diabetes.…”

Section: Introductionmentioning

confidence: 99%

Evidence of Cu(I) Coupling with Creatinine Using Cuprous Nanoparticles Encapsulated with Polyacrylic Acid Gel-Cu(II) in Facilitating the Determination of Advanced Kidney Dysfunctions

Kalasin

Sangnuang

Khownarumit

et al. 2020

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

An electrochemical-based sensor created for creatinine detection has been developed for early point-of-care (POC) of diagnosis of renal illnesses. Useful information for the preventive diagnosis and clinical treatments of congenital disorders of creatinine mechanism, advanced liver and kidney diseases, and renal dysfunction can be obtained by the noninvasive evaluation of the creatinine levels in urine. The direct detection of creatinine can be achieved using the modified nanocomposite of cuprous nanoparticles encapsulated by polyacrylic acid (PAA) gel-Cu(II) fabricating on a screen-printed carbon electrode. Here, we report that the degree of kidney dysfunction failure can be determined by an amount of Cu(I) bound with the creatinine through the adsorptive mechanism on the modified electrode. Under cyclic voltammetry scans, the amount of creatinine was measured from the adsorptive signals of the redox peak current identifying the Cu(I)–creatinine complex with a natural logarithm of the creatinine concentration ranging from 200 μM to 100 mM. For this detection range, the theoretical calculation was postulated to describe experimental behaviors of the adsorptive mechanism as creatinine diffused to adsorb on the composite-modified electrode to reduce oxidized copper nanoparticles and transformed to Cu(II)–creatinine complexes. Interestingly, there was evidence that anodic peak potentials had been reduced in magnitudes and shifted negatively by natural logarithm during the formation of the Cu(I)–creatinine complex. For practical usage in POC technology, the creatinine detection in interference was carried out using differential pulse voltammetry to solely determine faradaic currents of creatinine–copper formation. With the interference of urea, glucose, ascorbic acid, glycine, and uric acid in artificial urine, the sensor showed promising results of the interference-free determination with 99.4% sensitivity efficiency, whereas for human urine interference, this sensor showed 85% sensitivity efficiency in detecting creatinine. This shows that this composite-modified sensor (PAA gel-Cu(II)/Cu2O NPs) has great potential for use in the next-generation devices for creatinine sensing to determine the progression in kidney dysfunctions.

show abstract

A fast, sensitive, single-step colorimetric dipstick assay for quantifying ascorbic acid in urine

Cited by 12 publications

References 25 publications

A ninety-six well plate as headspaces with moist starch indicator paper as a cover for the determination of ascorbic acid by iodate oxidation and formation of volatile iodine

A ninety-six well plate as headspaces with moist starch indicator paper as a cover for the determination of ascorbic acid by iodate oxidation and formation of volatile iodine

Vitamin C—Sources, Physiological Role, Kinetics, Deficiency, Use, Toxicity, and Determination

Evidence of Cu(I) Coupling with Creatinine Using Cuprous Nanoparticles Encapsulated with Polyacrylic Acid Gel-Cu(II) in Facilitating the Determination of Advanced Kidney Dysfunctions

Contact Info

Product

Resources

About