A fast, ultrasensitive SERS immunoassay to detect SARS-CoV-2 in saliva

Mohammadi, Moein; Antoine, Delphine; Vitt, Madison; Dickie, Julia Marie; Jyoti, Sharmin Sultana; Wall, J. Gerard; Johnson, Patrick A.; Wawrousek, Karen

doi:10.1016/j.aca.2022.340290

Cited by 27 publications

(16 citation statements)

References 63 publications

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“…While both of the present scFvs retained binding to spike protein from the Delta variant virus, neither scFv exhibited detectable binding of Omicron spike protein. This contrasts with scFv 3 which was also isolated from the biopanning and bound Alpha, Beta, Delta and Omicron spike proteins with similar affinity (Mohammadi et al, 2022), but failed to inhibit the RBD-ACE2 interaction in this study. The inability of the Wuhan-Hu-1-and Delta-binding scFvs 10 and D4 to detect Omicron spike protein mirrors clinical experience, with the ongoing emergence of virus variants which are resistant to detection and/or neutralisation by antibodies protective against earlier strains (Focosi et al, 2021).…”

Section: Discussioncontrasting

confidence: 70%

Isolation of SARS-CoV-2-blocking recombinant antibody fragments and characterisation of their binding to variant spike proteins

Antoine

Mohammadi

McDermott

et al. 2022

Front. Nanotechnol.

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COVID-19 is a severe acute respiratory disease caused by SARS-CoV-2. From its initial appearance in Wuhan, China in 2019, it developed rapidly into a global pandemic. In addition to vaccines, therapeutic antibodies play an important role in immediately treating susceptible individuals to lessen severity of the disease. In this study, phage display technology was utilised to isolate human scFv antibody fragments that bind the receptor-binding domain (RBD) of SARS-CoV-2 Wuhan-Hu-1 spike protein. Of eight RBD-binding scFvs isolated, two inhibited interaction of RBD with ACE2 protein on VeroE6 cells. Both scFvs also exhibited binding to SARS-CoV-2 Delta variant spike protein but not to Omicron variant spike protein in a Raman spectroscopy immunotest. The study demonstrates the potential of recombinant antibody approaches to rapidly isolate antibody moieties with virus neutralisation potential.

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Section: Discussioncontrasting

confidence: 70%

Isolation of SARS-CoV-2-blocking recombinant antibody fragments and characterisation of their binding to variant spike proteins

Antoine

Mohammadi

McDermott

et al. 2022

Front. Nanotechnol.

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“…The information obtained on the adsorption on metal nanoparticles, on the chemical species linked to metal, on the molecular sites directly involved in the chemical interaction, and on the structural and electronic modifications of the adsorbed molecule are of fundamental importance in view of the present and future applications of this spectroscopic technique. In this respect, the most recent SERS investigations in the field of medical diagnosis are of particular relevance [ 65 , 66 , 67 , 68 , 69 ].…”

Section: Discussionmentioning

confidence: 99%

Can DFT Calculations Provide Useful Information for SERS Applications?

Muniz‐Miranda

Menziani

et al. 2023

Molecules

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Density functional theory (DFT) calculations allow us to reproduce the SERS (surface-enhanced Raman scattering) spectra of molecules adsorbed on nanostructured metal surfaces and extract the most information this spectroscopy is potentially able to provide. The latter point mainly concerns the anchoring mechanism and the bond strength between molecule and metal as well as the structural and electronic modifications of the adsorbed molecule. These findings are of fundamental importance for the application of this spectroscopic technique. This review presents and discusses some SERS–DFT studies carried out in Italy as a collaboration between the universities of Modena and Reggio-Emilia and of Florence, giving an overview of the information that we can extract with a combination of experimental SERS spectra and DFT modeling. In addition, a selection of the most recent studies and advancements on the DFT approach to SERS spectroscopy is reported with commentary.

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“…Experimental results also showed that the biosensor possessed low detection limit and wide linear range for the COVID-19 antigen assay (Table S1). [43][44][45][46][47]…”

Section: Detection Of Rbd Of Sars-cov-2 Spike Glycoproteinmentioning

confidence: 99%

“…The experimental results also showed that the biosensor possessed a low detection limit and wide linear range for the COVID-19 antigen assay (Table S1 †). [43][44][45][46][47] 3.6. Selectivity, stability, and reproducibility of the biosensor The selectivity of the biosensor was evaluated by measurements of the target RBD and various potential proteins including immunoglobulin G (IgG), immunoglobulin M (IgM), human epidermal growth factor receptor 2 (HER2), carbohydrate antigen 15-3 (CA15-3), carbohydrate antigen 12-5 (CA12-5), carcinoembryonic antigen (CEA), and alpha fetoprotein (AFP) (Fig.…”

Section: Detection Of Rbd Of Sars-cov-2 Spike Glycoproteinmentioning

confidence: 99%