A fluorescent <i>τ</i>-probe: quantitative imaging of ultra-trace endogenous hydrogen polysulfide in cells and <i>in vivo</i>

Yang, Fan; Gao, He; Li, Shanshan; An, Ruibing; Sun, Xiaoyang; Kang, Bin; Xu, Jing‐Juan; Chen, Hong‐Yuan

doi:10.1039/c8sc01879k

Cited by 41 publications

(18 citation statements)

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“…Maximum human plasma concentrations of DOXY are usually ranging from 1.5 to 7.0 µg/mL or from 3 to 14.6 µM [45,46]. Concentrations of endogenously produced polysulfides in HeLa cells are ~0–120 nM [47] and H 2 S concentration can be higher than 1 µM [48]. However, in the very local environment presence of 1 molecule in 1000 nm 3 gives raise of 1.65 mM H 2 S or polysulfide concentration.…”

Section: Discussionmentioning

confidence: 99%

Sulfide (Na2S) and Polysulfide (Na2S2) Interacting with Doxycycline Produce/Scavenge Superoxide and Hydroxyl Radicals and Induce/Inhibit DNA Cleavage

et al. 2019

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Doxycycline (DOXY) is an antibiotic routinely prescribed in human and veterinary medicine for antibacterial treatment, but it has also numerous side effects that include oxidative stress, inflammation, cancer or hypoxia-induced injury. Endogenously produced hydrogen sulfide (H2S) and polysulfides affect similar biological processes, in which reactive oxygen species (ROS) play a role. Herein, we have studied the interaction of DOXY with H2S (Na2S) or polysulfides (Na2S2, Na2S3 and Na2S4) to gain insights into the biological effects of intermediates/products that they generate. To achieve this, UV-VIS, EPR spectroscopy and plasmid DNA (pDNA) cleavage assay were employed. Na2S or Na2S2 in a mixture with DOXY, depending on ratio, concentration and time, displayed bell-shape kinetics in terms of producing/scavenging superoxide and hydroxyl radicals and decomposing hydrogen peroxide. In contrast, the effects of individual compounds (except for Na2S2) were hardly observable. In addition, DOXY, as well as oxytetracycline and tetracycline, interacting with Na2S or other studied polysulfides reduced the •cPTIO radical. Tetracyclines induced pDNA cleavage in the presence of Na2S. Interestingly, they inhibited pDNA cleavage induced by other polysulfides. In conclusion, sulfide and polysulfides interacting with tetracyclines produce/scavenge free radicals, indicating a consequence for free radical biology under conditions of ROS production and tetracyclines administration.

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Section: Discussionmentioning

confidence: 99%

Sulfide (Na2S) and Polysulfide (Na2S2) Interacting with Doxycycline Produce/Scavenge Superoxide and Hydroxyl Radicals and Induce/Inhibit DNA Cleavage

et al. 2019

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“…The lifetimes of the micelles M1, M2, and M3 were determined by using an FLS980 Spectrometer [40,41]. First, 3 mL of PtTFPP micelles were placed in a quartz cuvette, and the phosphorescence lifetime of the luminescent particles was measured respectively under air and nitrogen conditions.…”

Section: Lifetime Determinationmentioning

confidence: 99%

Extracellular Oxygen Sensors Based on PtTFPP and Four-Arm Block Copolymers

Qiao

Pan

et al. 2019

Applied Sciences

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Featured Application: Nanosensors for extracellular oxygen-sensing.Abstract: Three four-arm amphiphilic block copolymers with different chain lengths, consisting of a hydrophilic chain of polyethylene glycol (PEG) and hydrophobic segment of polycaprolactam (PCL), were synthesized and used to encapsulate the high-efficient and hydrophobic oxygen probe of platinum(II)-5,10,15,20-tetrakis-(2,3,4,5,6-pentafluorophenyl)-porphyrin (PtTFPP) to form polymer micelles. This approach enabled the use of PtTFPP in aqueous solution for biosensing. Experimental results demonstrated that the particle sizes of these nano-oxygen sensors between 40.0 and 203.8 nm depend on the structures of block copolymers. PtTFPP in these micelles showed an effective quantum yield under nitrogen environment, ranging from 0.06 to 0.159. The new sensors are suitable for analyzing dissolved oxygen concentrations in the range of 0.04-39.3 mg/L by using the linear Stern-Volmer equation at room temperature. In addition, it has been shown that these sensors are capable of in situ monitoring the dissolved oxygens in the culture medium of E. coli and Romas cells during the respiration process, and distinguishing the drug activity of antibiotic ampicillin from that of antimycin A. This study showed that the use of these nanostructured multi-arm block copolymer micelles can achieve efficient biological applications without specific structural modification of the hydrophobic PtTFPP probe, which is expected to have broad prospects.

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“…Due to the high reactivity, low concentration, and short lifetime of H 2 S n and O 2 •– in biological systems, it is of great significance to develop suitable analytical methods to trap them and assess the real-time existence of these reactive species. Fluorescence imaging can meet the above requirements, for it can offer in real-time, in situ and noninvasive imaging analysis. − Despite the fact that the fluorescent probes for imaging of O 2 •– and H 2 S n in cells have been elegantly developed. ,− However, the separate detection of H 2 S n and O 2 •– in the same detection system always leads to unsatisfactory spectral overlap, nonhomogeneous distribution, and furthermore, the test conditions are coordinated differently . , To overcome these limitations, a probe can be conceived to offer a synchronous response to multispecies under the same test condition. In the past years, some fluorescent probes for the simultaneous and sequential detection of multiple analytes are reported. − We hope the synchronous response of both H 2 S n and O 2 •– with a single probe will better clarify the cross-talk between the two biological species in the regulation of signal transduction and redox homeostasis. − Therefore, the development of multiresponse fluorescent probe is of great urgency and expectation.…”

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confidence: 99%

Sequential Detection of Superoxide Anion and Hydrogen Polysulfides under Hypoxic Stress via a Spectral-Response-Separated Fluorescent Probe Functioned with a Nitrobenzene Derivative

et al. 2019

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Chronic hypoxic stress disrupts the intracellular redox homeostasis, leads to a series of physiological dysfunction, and finally results in many diseases including cancer and inflammatory and cardiovascular diseases. The intracellular redox status is related to the homeostasis between reactive oxygen species (ROS) and cellular antioxidant species. Superoxide anion (O 2 •−) is considered to be a precursor of ROS. As a member of reactive sulfur species, hydrogen polysulfides (H 2 S n) are a class of antioxidants in cells, which act as an important regulator for the intracellular redox state. Therefore, trapping the cross-talk of O 2 •− and H 2 S n is a benefit for further understanding the physiological and pathological effects. Herein, we conceive a fluorescent probe HCy-ONO for sequential detection of O 2 •− and H 2 S n in cells and in mouse models. Based on a tandem reaction, the probe HCy-ONO can be used to detect O 2 •− and H 2 S n in different fluorescence collection windows without spectral overlap interference with limits of detection 90 and 100 nM, respectively. The strategy affords high sensitivity and selectivity for our detection in living cell models under continuous hypoxic and intermittent hypoxic conditions, revealing the reason for ischemia-reperfusion injury. Moreover, the probe can distinguish the inflamed tissue from normal tissue in acute peritonitis mouse model. Finally, our probe is successfully applied for imaging of O 2 •− and H 2 S n in the SH-SY5Y tumor-bearing mouse model, which is helpful to elucidate the physiological and pathological processes. These data demonstrated that different hypoxic status lead to different concentrations between H 2 S n and O 2 •− .

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A fluorescent τ-probe: quantitative imaging of ultra-trace endogenous hydrogen polysulfide in cells and in vivo

Abstract: A fluorescent τ-probe was demonstrated to quantitate ultra-trace endogenous hydrogen sulfide (H2Sn) within cells and zebrafish through changes of fluorescence lifetime.

Cited by 41 publications

References 50 publications

Sulfide (Na2S) and Polysulfide (Na2S2) Interacting with Doxycycline Produce/Scavenge Superoxide and Hydroxyl Radicals and Induce/Inhibit DNA Cleavage

Sulfide (Na2S) and Polysulfide (Na2S2) Interacting with Doxycycline Produce/Scavenge Superoxide and Hydroxyl Radicals and Induce/Inhibit DNA Cleavage

Extracellular Oxygen Sensors Based on PtTFPP and Four-Arm Block Copolymers

Sequential Detection of Superoxide Anion and Hydrogen Polysulfides under Hypoxic Stress via a Spectral-Response-Separated Fluorescent Probe Functioned with a Nitrobenzene Derivative

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