A Fluorescent Polymer for Patterning of Mesenchymal Stem Cells

You, Jungmok; Heo, June Seok; Lee, Jiyea; Kim, Han Soo; Kim, Hyun Ok; Kim, Eunkyoung

doi:10.1021/ma802722q

Cited by 27 publications

(34 citation statements)

References 47 publications

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“…1B, C). 14 Traditional tissue culture plates with -COOH and -OH, (B) hydrophobic DTOPV-coated plates with only tertiary amine, (C) DTOPV-UV with -COOH produced by UV exposure to DTOPV, (D) negatively and positively charged plates with -COOH, -OH, and -NH 2 , and (E) amine-coated plates. The tissue culture plates with various chemical groups were tested for efficacy for enhancing enucleation of cultured erythroblasts in suspension.…”

Section: Methodsmentioning

confidence: 99%

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Enhanced Production of Red Blood Cells in Suspension by Electrostatic Interactions with Culture Plates

Baek

You

Kim

et al. 2010

Tissue Engineering Part C: Methods

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Enucleation of erythroblasts, a critical step in the generation of red blood cells (RBCs), occurs at a low rate without cocultured stromal cells. Previously, the surface properties of the cell culture plate were not considered in the enucleation process, because the cells exist in suspension. Here, we show that a significantly higher rate of enucleation of erythroblasts occurred on the positively charged plates than on the negatively charged surfaces or the both negatively and positively charged plates. Also, the negatively and positively charged plate group showed a significantly higher enucleation than did the hydrophobic plates. Therefore, the plates fully coated with amine groups generated 1.88 times more enucleated RBCs than did the hydrophobic plates. This study suggests an important insight into the effect of surface characteristics of cell culture plates on suspension cell culture. Further, this simple and inexpensive procedure could contribute to a more efficient RBC production system, eliminating the need for robust and expensive coculture procedures.

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Section: Methodsmentioning

confidence: 99%

“…14 The static contact angles of sessile water drops on culture plates were measured at room temperature with a CAM 101 contact angle measurement instrument (KSV Instruments Ltd.). The CAM 101 video capture and the captured image was investigated by axis-symmetric drop shape analysis.…”

Section: Comparison Of the Surface Characteristicsmentioning

confidence: 99%

Enhanced Production of Red Blood Cells in Suspension by Electrostatic Interactions with Culture Plates

Baek

You

Kim

et al. 2010

Tissue Engineering Part C: Methods

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“…Recently, we reported direct photopatterning approaches for functional polymers to avoid decomposition of the functional group in polymer structure during the patterning step [ 20 – 22 ]. Leveraging simple and direct photo-patterning process, we have developed a highly fluorescent and biocompatible p -phenylene vinylene (PPV) polymer pattern film that allows stem cell micropatterns [ 23 ]. This enables us to easily detect the location of cells or other biomaterials without the labeling cells because the pattern is fluorescent.…”

Section: Introductionmentioning

confidence: 99%

Direct photo-patterning on anthracene containing polymer for guiding stem cell adhesion

et al. 2016

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BackgroundVarious micropatterned surfaces capable of guiding the selective adhesion of biomolecules such as proteins and cells are of great interests in biosensor, diagnostics, drug screening, and tissue engineering. In this study, we described a simple photo-patterning method to prepare micro-patterned films for stem cell patterning using anthracene containing polymers (PMAn). This micro patterned polymer film was prepared by the facile photo-reaction of anthracene units in polymer backbone structure.ResultsThe UV irradiation of PMAn through a photomask resulted in the quenching of fluorescent intensity as well as the changes in surface wettability from hydrophobic to hydrophilic surface. As a result, UV exposed regions of PMAn film show lower fluorescent intensity as well as higher proliferation rate of mesenchymal stem cells (MSCs) than unexposed region of PMAn film. Furthermore, the selective MSC attachment was clearly observed in the UV exposed regions of PMAn film.ConclusionWe developed a simple cell patterning method with a fluorescent, biocompatible, and patternable polymer film containing anthracene units. This method provides a facile stem cell patterning method and could be extended to various patterning of biomaterials without labor-intensive preparation and no pre-treatment for complex interactions of cell-microenvironment.

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“…Dopants may be an anion, either small (e.g., Cl -, ClO4 -) or large (e.g., polystyrene sulfonate (PSS)) [14], [40], [41], [42]. [48] conducting biomaterials [49] polypyrrole PPy [C4H2NH]n 10 -7.5 x 10 3 modulate cellular activities [50], [51] nerve regeneration [52] biomedicine [53] biosensors [54] bacterial detection [55], [56], [57] poly(p-phenylene) PPP [C6H4]n 10 -3 -10 2 dental applications [58] cell alignment [59], [60], [61], [62] polyaniline PANI [C6H4NH]n 10 -2 -200 neural application [63] tissue engineering [64] biosensors [65], [66], [67], [68], [69] Reprinted with permission from Ref. 28.…”

Section: Electronically Conducting Polymers (Intrinsically Conductingmentioning

confidence: 99%

Conducting Polymers, Hydrogels and Their Composites: Preparation, Properties and Bioapplications

Tomczykowa

Płońska‐Brzezińska

2019

Preprint

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This review is focused on current state-of-the-art research on electroactive-based materials and their synthesis, as well as their physicochemical and biological properties. Special attention is paid to pristine intrinsically conducting polymers (ICPs) and their composites with other organic and inorganic components, well-defined micro- and nanostructures, and enhanced surface areas compared with those of conventionally prepared ICPs. Hydrogels, due to their defined porous structures and being filled with aqueous solution, offer the ability to increase the amount of immobilized chemical, biological or biochemical molecules. When other components are incorporated into ICPs, the materials form composites; in this particular case, they form conductive composites. The design and synthesis of conductive composites result in the inheritance of the advantages of each component and offer new features because of the synergistic effects between the components. The resulting structures of ICPs, conducting polymer hydrogels and their composites, as well as the unusual physicochemical properties, biocompatibility and multi-functionality of these materials, facilitate their bioapplications. The synergistic effects between constituents have made these materials particularly attractive as sensing elements for biological agents, and they also enable the immobilization of bioreceptors such as enzymes, antigen–antibodies, and nucleic acids onto their surfaces for the detection of an array of biological agents. Currently, these materials have unlimited applicability in biomedicine. In this review, we have limited discussion to three areas in which it seems that the use of ICPs and materials, including their different forms, are particularly interesting, namely, biosensors, delivery of drugs and tissue engineering.

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A Fluorescent Polymer for Patterning of Mesenchymal Stem Cells

Cited by 27 publications

References 47 publications

Enhanced Production of Red Blood Cells in Suspension by Electrostatic Interactions with Culture Plates

Enhanced Production of Red Blood Cells in Suspension by Electrostatic Interactions with Culture Plates

Direct photo-patterning on anthracene containing polymer for guiding stem cell adhesion

Conducting Polymers, Hydrogels and Their Composites: Preparation, Properties and Bioapplications

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