A fluorescent probe based on tryptophan-coated silver nanoclusters for copper (II) ions detection and bioimaging in cells

Li, Shaoqing; Li, Gufeng; Shi, Hongyu; Yang, Min; Tan, Wei; Wang, Hongbin; Yang, Wenrong

doi:10.1016/j.microc.2022.107222

Cited by 32 publications

(7 citation statements)

References 47 publications

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“…The precision of our protocol (especially at low concentrations) is inside the range (from 2% to 13%) of those found in the literature [35][36][37][38][39][40], that exploit fluorescence spectroscopy and photoactive materials. A comparative table with the literature is shown in the supporting information (Table S4).…”

Section: Real Water Testssupporting

confidence: 61%

Determination of Pb(II) Ions in Water by Fluorescence Spectroscopy Based on Silver Nanoclusters

et al. 2022

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In this work, a method to determine Pb(II) ions in model water is presented; the method is based on the fluorescence emission of a silver nanoclusters (AgNCs) colloidal solution, which is sensitive to lead ions. The presence of Pb(II) ions causes a photoemission enhancement of the AgNCs solution dependent on the pollutant concentration. The functional dependence is logarithmic in the range from 2.5 to 40 µM, and through the linearization of the calibration points, a linear function is determined and exploited for the extrapolation of the test Pb(II) concentrations with a precision estimated by relative standard deviation (RSD) ranging from 21% to 10% from the highest to the lowest Pb(II) quantity, respectively. Finally, inductively coupled plasma–optical emission spectroscopy (ICP-OES) successfully validated the described method. The accuracy of the method is also studied for intentionally polluted mineral waters, revealing the same trend of the model water: the lower the concentration, the higher the precision of the method.

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Section: Real Water Testssupporting

confidence: 61%

Determination of Pb(II) Ions in Water by Fluorescence Spectroscopy Based on Silver Nanoclusters

et al. 2022

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“…7A. 93 The reaction mechanisms were static quenching and charge transfer. Because of its good biocompatibility, it was possible to realize the visual detection of Cu 2+ in the cell culture environment.…”

Section: Applications Of Visual Detectionmentioning

confidence: 99%

Recent advances in the visual detection of ions and molecules based on gold and silver nanoclusters

et al. 2022

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“…Based on these requirements, in the last 10 years, several production processes have emerged, where only one smaller or larger molecule like proteins [ 8 , 9 ], peptides [ 10 ] and simple amino acids [ 11 , 12 , 13 ] are used for the reduction of metal ions (e.g., [AuCl 4 ] − , Ag + or Cu 2+ ), which in addition play a dominant role in the stabilization and functionalization of the metallic surface as well. In the case of amino acids, several articles can be found for tryptophan (Trp) [ 12 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ], tyrosine (Tyr) [ 21 , 22 ], histidine (His) [ 12 , 13 , 23 ], arginine (Arg) [ 24 ], or amino acids containing S donors like cysteine (Cys) or methionine (Met) [ 11 , 13 ]. Among these amino acids for Trp, it was confirmed that Au- [ 12 , 15 , 16 , 18 , 20 ], Ag- [ 14 , 19 ], and Cu-based [ 18 ] NCs can also be produced via a direct reduction of the metal ions with Trp without the application of any other agents.…”

Section: Introductionmentioning

confidence: 99%

“…In the case of amino acids, several articles can be found for tryptophan (Trp) [ 12 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ], tyrosine (Tyr) [ 21 , 22 ], histidine (His) [ 12 , 13 , 23 ], arginine (Arg) [ 24 ], or amino acids containing S donors like cysteine (Cys) or methionine (Met) [ 11 , 13 ]. Among these amino acids for Trp, it was confirmed that Au- [ 12 , 15 , 16 , 18 , 20 ], Ag- [ 14 , 19 ], and Cu-based [ 18 ] NCs can also be produced via a direct reduction of the metal ions with Trp without the application of any other agents. By comparing the experimental conditions optimized in these publications, it can be stated that predominantly the use of ligand excess (depending on the metal ions, these are in the range of a 2:1–20:0 molar ratio, 100–120 °C temperature, and basic conditions (pH = 11–12)) can be generally used to produce blue-emitting Trp-coated NCs.…”

Section: Introductionmentioning

confidence: 99%

Tryptophanhydroxamic Acid-Stabilized Ultrasmall Gold Nanoclusters: Tuning the Selectivity for Metal Ion Sensing

Gombár,

Ungor,

Szatmári

et al. 2024

Nanomaterials

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Sub-nanometer-sized gold nanoclusters (Au NCs) were prepared via the spontaneous reduction of [AuCl4]−- ions with a hydroxamate derivative of L-tryptophan (Trp) natural amino acid (TrpHA). The prepared TrpHA-Au NCs possess intense blue emission (λem = 470 nm; λex = 380 nm) with a 2.13% absolute quantum yield and 1.47 ns average lifetime. The Trp-stabilized noble metal NCs are excellent metal ion sensors for Fe3+, but in this work, we highlighted that the incorporation of the hydroxamate functional group with an excellent metal ion binding capability can tune the selectivity and sensitivity of these NCs, which is a promising way to design novel strategies for the detection of other metal ions as well. Moreover, their simultaneous identification can also be realized. By decreasing the sensitivity of our nano-sensor for Fe3+ (limit of detection (LOD) ~11 µM), it was clearly demonstrated that the selectivity for Cu2+-ions can be significantly increased (LOD = 3.16 µM) in an acidic (pH = 3–4) condition. The surface-bounded TrpHA molecules can coordinate the Cu2+ confirmed by thermodynamic data, which strongly generates the linking of the NCs via the Cu2+ ions in acidic pH, and a parallel fluorescence quenching occurs. In the case of Fe3+, the degree of quenching strongly depends on the metal ion concentration, and it only occurs when the NCs are not able to bind more Fe3+ (~10 µM) on the surface, causing the NCs’ aggregation.

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A fluorescent probe based on tryptophan-coated silver nanoclusters for copper (II) ions detection and bioimaging in cells

Cited by 32 publications

References 47 publications

Determination of Pb(II) Ions in Water by Fluorescence Spectroscopy Based on Silver Nanoclusters

Determination of Pb(II) Ions in Water by Fluorescence Spectroscopy Based on Silver Nanoclusters

Recent advances in the visual detection of ions and molecules based on gold and silver nanoclusters

Tryptophanhydroxamic Acid-Stabilized Ultrasmall Gold Nanoclusters: Tuning the Selectivity for Metal Ion Sensing

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