A fluoro-microbead guiding chip for simple and quantifiable immunoassay of cardiac troponin I (cTnI)

Song, Seung Yeon; Han, Yong Duk; Kim, Kangil; Yang, Sang Sik; Yoon, Hyun C.

doi:10.1016/j.bios.2011.02.036

Cited by 88 publications

(44 citation statements)

References 31 publications

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“…For FI, Song et al (2011) developed a fluoro-microbead guiding chip (FMGC)-based sandwich immunoassay for cTnI detection. The FMGC offers the capability of using a fluorescence microscope to count the number of beads bound in the immunosensing region directly.…”

Section: Fluorescence Immunoassaysmentioning

confidence: 99%

Diagnostics on acute myocardial infarction: Cardiac troponin biomarkers

Fathil

Arshad

Gopinath

et al. 2015

Biosensors and Bioelectronics

203

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Section: Fluorescence Immunoassaysmentioning

confidence: 99%

Diagnostics on acute myocardial infarction: Cardiac troponin biomarkers

Fathil

Arshad

Gopinath

et al. 2015

Biosensors and Bioelectronics

203

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“…To verify the applicability of RJPs as optical labels for the immunosensing of disease markers, a retroreflective sandwich immunoassay using SMJP for cTnI, an AMI-specific biomarker, was conducted. 25 Figure S4d). To the prepared cTnI immunosensing surface on the RQC, various concentrations of cTnI samples (0−100 ng/mL) and cTnI signaling antibodyconjugated SMJP probes (0.4 mg/mL) were sequentially applied ( Figure S4d).…”

Section: Acs Applied Materials and Interfacesmentioning

confidence: 90%

“…By counting the number of shining RJPs in the sensing zone using NIH ImageJ software, a linear dose−response curve for cTnI was obtained ( Figure 10b). 25 The calibration result yielded a cTnI detection range from 0.01 to 100 ng/mL. The limit of detection for cTnI immunosensing, calculated as 3.3 times the standard deviation of the background signal divided by the slope of the calibration curve based on the ICH guidelines, was as low as 0.05 ng/mL.…”

Section: Acs Applied Materials and Interfacesmentioning

confidence: 99%

Retroreflective Janus Microparticle as a Nonspectroscopic Optical Immunosensing Probe

Han

Kim

Park

et al. 2016

ACS Appl. Mater. Interfaces

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We developed retroreflective Janus microparticles (RJPs) as a novel optical immunosensing probe for use in a nonspectroscopic retroreflection-based immunoassay. By coating the metals on the hemispherical surface of silica particles, highly reflective RJPs were fabricated. On the basis of the retroreflection principle, the RJPs responded to polychromatic white light sources, in contrast to conventional optical probes, which require specific monochromatic light. The retroreflection signals from RJPs were distinctively recognized as shining dots, which can be intuitively counted using a digital camera setup. Using the developed retroreflective immunosensing system, cardiac troponin I, a specific biomarker of acute myocardial infarction, was detected with high sensitivity. On the basis of the demonstrated features of the retroreflective immunosensing platform, we expect that our approach may be applied for various point-of-care-testing applications.

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“…For troponin I detection, fluoro-microbead guiding chip (FMGC) performing by an optical immunoassay has been reported. 53 In 2011, Song and coworkers developed an optical immunoassay for cTnI. Antibody-tagged fluoro-microbeads were used to perform a sandwich immunoassay.…”

Section: Fluorescence-based Biosensors For Cardiac Troponin Detectionmentioning

confidence: 99%

“…9 avidin-biotin couple was used to enhance the signal. 53 DTSP, 3,3′-dithiobis-propionic acid N-hydroxysuccinimide ester; SAM, self-assembled monolayer.…”

Section: Fluorescence-based Biosensors For Cardiac Troponin Detectionmentioning

confidence: 99%