A functional polymorphism rs11614913 in microRNA-196a2 is associated with an increased risk of colorectal cancer although not with tumor stage and grade

Abstract

Abstract. A C/T polymorphism (rs11614913) was identified in the microRNA (miRNA) 196a2 (miR-196a2) gene and was implicated in the susceptibility to cancer. Numerous studies have investigated its association with the risk of colorectal cancer (CRC). However, the results were inconsistent and inconclusive. The present meta-analysis was conducted based on the results of six published case-control studies comprising 1,754 cases and 2,430 controls (up to November, 2012). Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated for the allelic and genotypic comparisons following the co-dominant, dominant and recessive genetic models. The Chi-square-based Q-test was used to assess heterogeneity. Egger's test and inverted funnel plots were used to investigate publication bias. Subgroup analysis was also performed. The results demonstrated that almost all the genetic models (except the model of CT vs. TT) indicated a significant association between rs11614913 polymorphism and CRC risk. The subgroup analysis in an Asian population also demonstrated similar results. However, there was no significant association of miR-196a2 rs11614913 polymorphism with the clinical characteristics of CRC patients. Our results confirmed the association of the polymorphism rs11614913 with the risk of CRC, but not with tumor stage and grade.

Deciphering the Variants Located in the MIR196A2, MIR146A, and MIR423 with Type-2 Diabetes Mellitus in Pakistani Population

“…They also reported a higher frequency of CC genotype (49%) in patients as compared to control (38%), while the genotype GG was more common in the control group (17%) as compared to patients (10%). The previous study has also shown that the two common variants: rs2910164 G/C, rs11614913 T/C were associated with the development of several diseases including type 2 diabetes mellitus (T2DM) [ 52 , 53 , 54 , 55 , 56 ].…”

MicroRNA gene polymorphisms and the risk of colorectal cancer

“…Some meta-analysis studies evaluated the correlation between one miRNA and a single cancer susceptibility. Wang et al confirmed the association of the polymorphism, miR-196a2 rs11614913, with the risk of CRC, but not with tumor stage and grade ( 43 ). Some meta-analysis studies evaluated the correlation between one miRNA and a variety of cancer susceptibilities.…”

MicroRNA-196a2 Biomarker and Targetome Network Analysis in Solid Tumors

“…1). Reports have demonstrated that this particular polymorphism could alter mature miR196a2 expression and binding ability to target mRNAs [13,15]. This was not predicted by the bioinformatics tools based on searching for perfect Watson-Crick pairing between the miRNAs' seed sequence and the target mRNA sequence alone, or in combination with other rules, such as evolutionary conservation criteria to predict miRNA target sites [16].…”

“…Altered miR-196a2 expression levels have been reported in various types of cancer [10,11]. High expression levels of mature miR-196a2 were found to promote the migration and invasion of cancer cells in vitro [12,13].…”