A gamma 3 hinge region probe: first specific human immunoglobulin subclass probe

Abstract

We report the first specific human immunoglobulin subclass probe which was obtained by subcloning the gamma 3 hinge region. This specific y3 probe allowed us to identify with certainty the Cy3 gene on Southern genomic blots, to describe the first Cy3 restriction fragment length polymorphism (EZZ y3 RF) and to show that an IgG3 selective deficiency, previously described serologically, was not due to a deletion of the Cy3 gene. Such a probe should be particularly useful for screening libraries from individuals with IgG3 immunodeficiencies or presenting unusual Cy3 genes and, consequently, for studying the Cy gene evolution.

Subclass restriction pattern of antigen-specific antibodies in donors with defective expression of IgG or IgA subclass heavy chain constant region genes

“…I ngiml). implying a regulatory nature of the defect, as suggested also by the presence of the C,3 gene in the BAR family (27).…”

Human IgE mRNA expression by peripheral blood lymphocytes stimulated with interleukin 4 and pokeweed mitogen

“…The probe for human C, chain is a 2.0-kb Eco RI -Hind I11 fragment isolated from plasmid pSH3Cy3 [27] …”

IGHV3-Associated Restriction Fragment Length Polymorphisms Confer Susceptibility to Bullous Pemphigoid

“…1. Polymorphisms of the IGHG genes using enzyme restriction-digested DNA obtained from BP patients and controls and pSH3CÁ3, a IGHV3 probe which cross-hybridizes with the other IGHG genes [14]. The molecular weights in kilobases (kb) of the analyzed DNA fragments are indicated.…”

“…The pSH3CÁ3 probe, a 2-kb SacI-SphI fragment encompassing the hinge and the exons 2 and 3 of the IGHG3 gene [14][15][16] which cross-hybridizes with the other IGHG genes (i.e., gamma 1, gamma 2, gamma 4, and pseudogamma genes) was used to analyze the RFLP of the IGH constant locus. The VH26-8 probe was kindly provided by T.H.…”