A genome-wide scan for quantitative trait loci affecting limb bone lengths and areal bone mineral density of the distal femur in a White Duroc × Erhualian F2 population

Mao, Huirong; Guo, Yuanmei; Yang, Guoqiang; Yang, Bin; Ren, Jun; Liu, Sanfeng; Ai, Huashui; Ma, Junwu; Brenig, Bertram; Huang, Lusheng

doi:10.1186/1471-2156-9-63

Cited by 19 publications

(24 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Quantitative trait loci for leg weakness in pigs ian intercross (Mao et al, 2008). The co-localization of the QTL for leg weakness-related traits and limb bone length was also observed on SSC7, on which the QTL for front gait score at 223 d was the most significant QTL detected in this study.…”

mentioning

confidence: 67%

A whole genome scan for quantitative trait loci for leg weakness and its related traits in a large F2 intercross population between White Duroc and Erhualian1

Guo

Ren

et al. 2009

Journal of Animal Science

View full text Add to dashboard Cite

To detect QTL for leg weakness and its related traits in pigs, a total of 1,484 F 2 pigs were recorded for leg (at 76 and 213 d) and gait scores (at 153 and 223 d) in a White Duroc × Erhualian intercross. The length and weight of the biceps brachii muscle were measured after slaughter at 240 d. A genome scan was performed with 183 microsatellite markers in the population. A total of 42 QTL were detected, including 16 at the 1% genome-wide significant level and 6 at the 5% genome-wide significant level. Thirty-eight of the 42 QTL showed significant additive effects, and 14 had significant dominance effects. At least 2 QTL were detected for each trait except for leg score at 76 d, for which no QTL was identified. Some of the QTL for leg and gait scores confirmed previous findings. Eighteen QTL were detected for weight and length of the biceps brachii muscle. To our knowledge, this was the first report about QTL for weight and length of the biceps brachii muscle in pigs. Two chromosome regions each on SSC4 and SSC7 showed significant and multiple associations with both leg weakness and growth of the biceps brachii muscle, which are worthwhile for further investigation. ABSTRACT: To detect QTL for leg weakness and its related traits in pigs, a total of 1,484 F 2 pigs were recorded for leg (at 76 and 213 d) and gait scores (at 153 and 223 d) in a White Duroc × Erhualian intercross. The length and weight of the biceps brachii muscle were measured after slaughter at 240 d. A genome scan was performed with 183 microsatellite markers in the population. A total of 42 QTL were detected, including 16 at the 1% genome-wide significant level and 6 at the 5% genome-wide significant level. Thirty-eight of the 42 QTL showed significant additive effects, and 14 had significant dominance effects. At least 2 QTL were detected for each trait except for leg score at 76 d, for which no QTL was identified. Some of the QTL for leg and gait scores confirmed previous findings. Eighteen QTL were detected for weight and length of the biceps brachii muscle. To our knowledge, this was the first report about QTL for weight and length of the biceps brachii muscle in pigs. Two chromosome regions each on SSC4 and SSC7 showed significant and multiple associations with both leg weakness and growth of the biceps brachii muscle, which are worthwhile for further investigation.

show abstract

mentioning

confidence: 67%

A whole genome scan for quantitative trait loci for leg weakness and its related traits in a large F2 intercross population between White Duroc and Erhualian1

Guo

Ren

et al. 2009

Journal of Animal Science

View full text Add to dashboard Cite

show abstract

“…Therefore, the disorder of bone and cartilage are thought to be connected. However, both BMD and OC are reported to have a genetic background and are directly related to each other through genetic factors (Jørgensen and Nielsen, 2005;Kadarmideen and Janss, 2005;Mao et al, 2008). Anyway, no studies are found in pigs to address the relationship between BMD and OC.…”

Section: Discussionmentioning

confidence: 89%

Molecular characterization and methylation study of matrix gla protein in articular cartilage from pig with osteochondrosis

Laenoi

Uddin

Çınar

et al. 2010

Gene

View full text Add to dashboard Cite

“…The genomic region harboring sPPARD G32E is of great interest in pig genetics, because significant QTL for diverse traits related to growth, carcass length, skeletal morphology and fat deposition have been consistently evidenced in this region using different crosses between Chinese indigenous pigs and Western commercial breeds [45]–[50]. Given that sPPARD serves as a crucial and multifaceted determinant of diverse biological functions including lipid metabolism, cartilage development, chondrocyte proliferation and differentiation [51]–[54], sPPARD is a strong candidate of the multiple significant pleiotropic QTL on SSC7.…”

Section: Discussionmentioning

confidence: 99%

The G32E Functional Variant Reduces Activity of PPARD by Nuclear Export and Post-Translational Modification in Pigs

Duan

Brenig

et al. 2013

PLoS ONE

Self Cite

View full text Add to dashboard Cite

Peroxisome proliferator-activated receptor beta/delta (PPARD) is a crucial and multifaceted determinant of diverse biological functions including lipid metabolism, embryonic development, inflammatory response, wound healing and cancer. Recently, we proposed a novel function of porcine PPARD (sPPARD) in external ear development. A missense mutation (G32E) in an evolutionary conservative domain of sPPARD remarkably increases external ear size in pigs. Here, we investigated the underlying molecular mechanism of the causal mutation at the cellular level. Using a luciferase reporter system, we showed that the G32E substitution reduced transcription activity of sPPARD in a ligand-dependent manner. By comparison of the subcellular localization of wild-type and mutated sPPARD in both PK-15 cells and pinna cartilage-derived primary chondrocytes, we found that the G32E substitution promoted CRM-1 mediated nuclear exportation of sPPARD. With the surface plasmon resonance technology, we further revealed that the G32E substitution had negligible effect on its ligand binding affinity. Finally, we used co-immunoprecipitation and luciferase reporter assays to show that the G32E substitution greatly reduced ubiquitination level by blocking ubiquitination of the crucial A/B domain and consequently decreased transcription activity of sPPARD. Taken together, our findings strongly support that G32E is a functional variant that plays a key role in biological activity of sPPARD, which advances our understanding of the underlying mechanism of sPPARD G32E for ear size in pigs.

show abstract

A genome-wide scan for quantitative trait loci affecting limb bone lengths and areal bone mineral density of the distal femur in a White Duroc × Erhualian F2 population

Cited by 19 publications

References 31 publications

A whole genome scan for quantitative trait loci for leg weakness and its related traits in a large F2 intercross population between White Duroc and Erhualian1

A whole genome scan for quantitative trait loci for leg weakness and its related traits in a large F2 intercross population between White Duroc and Erhualian1

Molecular characterization and methylation study of matrix gla protein in articular cartilage from pig with osteochondrosis

The G32E Functional Variant Reduces Activity of PPARD by Nuclear Export and Post-Translational Modification in Pigs

Contact Info

Product

Resources

About