A glassy carbon electrode modified with reduced graphene oxide and gold nanoparticles for electrochemical aptasensing of lipopolysaccharides from Escherichia coli bacteria

Pourmadadi, Mehrab; Shayeh, Javad Shabani; Omidi, Meisam; Yazdian, Fatemeh; Alebouyeh, Masoud; Tayebi, Lobat

doi:10.1007/s00604-019-3957-9

Cited by 81 publications

(38 citation statements)

References 35 publications

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“…There are two reasons for this increase. The first one is that the excellent electrical properties of graphene itself improve the electron transfer rate ( Pourmadadi et al, 2019 ). The second is that the lamellar structure of graphene greatly enhances the electrochemically active area ( Gupta et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Graphene-Assisted Sensor for Rapid Detection of Antibiotic Resistance in Escherichia coli

Sun

2021

Front. Chem.

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In recent years, antibiotic-resistant bacteria caused by antibiotic abuse in the medical industry have become a new environmental pollutant that endangers public health. Therefore, it is necessary to establish a detection method for evaluating drug-resistant bacteria. In this work, we used Escherichia coli as a target model and proposed a method to evaluate its drug resistance for three antibiotics. Graphene dispersion was used to co-mix with E. coli cells for the purpose of increasing the current signal. This electrochemical-based sensor allows the evaluation of the activity of E. coli on the electrode surface. When antibiotics were present, the electrocatalytic reduction signal was diminished because of the reduced activity of E. coli. Based on the difference in the electrochemical reduction signal, we can evaluate the antibiotic resistance of different E. coli strains.

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Section: Resultsmentioning

confidence: 99%

Graphene-Assisted Sensor for Rapid Detection of Antibiotic Resistance in Escherichia coli

Sun

2021

Front. Chem.

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“…Compared with GO, RGO has higher electrical conductivity, better ductility, thermal stability, and has received more attention in the biological analysis [96]. For example, Pourmadadi et al modified aptamers on the surface of a glassy carbon electrode (GCE) via RGO and AuNPs, which were successfully used in the analysis of serum of patients and normal people [53]. In another case, the research by Yazdian et al used RGO-Au NPs to modify electrodes to immobilize thiolated aptamer that specifically binds to endotoxin [54].…”

Section: Lpsmentioning

confidence: 99%

Aptamer-based biosensors for the diagnosis of sepsis

Liu

Han

et al. 2021

J Nanobiotechnol

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Sepsis, the syndrome of infection complicated by acute organ dysfunction, is a serious and growing global problem, which not only leads to enormous economic losses but also becomes one of the leading causes of mortality in the intensive care unit. The detection of sepsis-related pathogens and biomarkers in the early stage plays a critical role in selecting appropriate antibiotics or other drugs, thereby preventing the emergence of dangerous phases and saving human lives. There are numerous demerits in conventional detection strategies, such as high cost, low efficiency, as well as lacking of sensitivity and selectivity. Recently, the aptamer-based biosensor is an emerging strategy for reasonable sepsis diagnosis because of its accessibility, rapidity, and stability. In this review, we first introduce the screening of suitable aptamer. Further, recent advances of aptamer-based biosensors in the detection of bacteria and biomarkers for the diagnosis of sepsis are summarized. Finally, the review proposes a brief forecast of challenges and future directions with highly promising aptamer-based biosensors.

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“…Nitrite is an electroactive substance with redox reaction at the surface of carbon electrodes, but the electron exchange rate is usually poor, which causes great limitations in the detection sensitivity [17]. It is reported that many nanostructured materials are excellent electrode materials with high conductivity [18][19][20]. Therefore, it is a good alternative approach to modify electrodes with these nanomaterials.…”

Section: Introductionmentioning

confidence: 99%

Mesoporous CoOx/C Nanocomposites Functionalized Electrochemical Sensor for Rapid and Continuous Detection of Nitrite

et al. 2021

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Nitrite is widespread in the environment, and is frequently used as an additive to extend the shelf life of meat products. However, the excess intake of nitrite can be harmful to human health. Hence, it is very important to know and control the content of nitrite in foodstuffs. In this work, by the means of self-assembly induced by solvent evaporation, we used the amphiphilic PEO-b-PS diblock copolymers resol and cobalt nitrate as a template to synthesize ordered mesoporous CoOx/C nanocomposites. Then, the CoOx/C nanocomposites were modified on a glassy carbon electrode (GCE), which showed excellent sensitivity, good selectivity, and a wide detection range for nitrite. Through cyclic voltammetry and current–time techniques, the electrochemical performance of the GCE modified with CoOx/C nanocomposites was analyzed. Under the optimized conditions, we found that anodic currents were linearly related to nitrite concentrations with a regression equation of lp (µA) = 0.36388 + 0.01616C (R2 = 0.9987) from 0.2 µM to 2500 µM, and the detection limit was 0.05 µM. Furthermore, the electrochemical sensor behaved with high reproducibility and anti-interference ability towards various organic and inorganic ions, such as NO3−, SO42−, Cl−, COOH− (Ac−), Na+, K+, Mg2+, and NH4+. Our results indicated that these CoOx/C nanocomposites could be applied in electrochemical sensors for the rapid and sensitive detection of the food preservative nitrite.

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A glassy carbon electrode modified with reduced graphene oxide and gold nanoparticles for electrochemical aptasensing of lipopolysaccharides from Escherichia coli bacteria

Cited by 81 publications

References 35 publications

Graphene-Assisted Sensor for Rapid Detection of Antibiotic Resistance in Escherichia coli

Graphene-Assisted Sensor for Rapid Detection of Antibiotic Resistance in Escherichia coli

Aptamer-based biosensors for the diagnosis of sepsis

Mesoporous CoOx/C Nanocomposites Functionalized Electrochemical Sensor for Rapid and Continuous Detection of Nitrite

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