A gold nanoparticle-dye/poly(3-aminobenzylamine)/two dimensional MoSe2/graphene oxide electrode towards label-free electrochemical biosensor for simultaneous dual-mode detection of cancer antigen 15-3 and microRNA-21

Pothipor, Chammari; Jakmunee, Jaroon

doi:10.1016/j.colsurfb.2021.112260

Cited by 59 publications

(25 citation statements)

References 59 publications

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“…In order to further expand the application of graphene in electrochemical sensors, a lot of work has been carried out to further optimize the performance of graphene by means of doping other materials, chemical functionalization, and synthesis of composite materials ( Reddy et al, 2020 ). Gold is the most chemically stable metal, with good electrical conductivity and biocompatibility, and can be combined with biologically active proteins, enzymes, and other molecules without destroying their activity ( Karimi-Maleh et al, 2021a ; Wang et al, 2021 ; Zheng et al, 2021 ; Karimi-Maleh et al, 2022b ; Pothipor et al, 2022 ). Gold nanomaterials are often modified on the electrode surface as substrate materials to immobilize antigens or antibodies and provide a good channel environment for electron transfer on the electrode surface.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Detection of Carcinoembryonic Antigen via an Electrochemical Platform Fabricated by AuNPs/Streptavidin/Reduced Graphene Oxide

Guo

2022

Front. Chem.

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Tumor markers are one of the important indicators for early cancer diagnosis. As a new analytical method, electrochemical immunosensing analysis has the advantages of high sensitivity, good selectivity, and rapid detection, which is of great significance for the detection of tumor markers. In this work, an AuNP/reduced graphene oxide (AuNP/rGO) composite was synthesized. We used it for electrochemical sensor fabrication with the assistance of the biotin–streptavidin protein (SA) system to further amplify the signal to achieve sensitive detection of carcinoembryonic antigen (CEA). In addition, AuNPs have been incorporated due to their good electrical conductivity and biocompatibility, which can accelerate electron transfer at the electrode interface and improve the loading capacity to capture antibodies. The fabricated AuNPs/SA/rGO has a large working surface area and high material utilization ratio, which improves the catalytic capacity of H2O2 reduction and effectively amplifies the current signal. The linear range of the response current signal of the sensor toward the CEA concentration is 20 fg/ml to 200 ng/ml, and the limit of detection can achieve 6.2 fg/ml. In addition, the fabricated immunosensor has good reproducibility, selectivity, and stability.

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Section: Introductionmentioning

confidence: 99%

Highly Sensitive Detection of Carcinoembryonic Antigen via an Electrochemical Platform Fabricated by AuNPs/Streptavidin/Reduced Graphene Oxide

Guo

2022

Front. Chem.

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“…Superior electronic conductivity and high surface-to-volume ratio of the nanocomposite, are the key reason for more antibody and capture probe loading which further influenced enhanced peak current and selectivity for the target components. The LODs of 0.14 U ml −1 and 1.2 fM for CA 15-3 and miRNA-21, respectively were achieved in this work and further was demonstrated for the target detection from simulated serum samples 37 which is very important as mentioned in the introduction section of this review that along with miRNAs, other proteins and hormones which needed to be tested for the DCIS condition makes the sensor suitable to be an alternative diagnostic tool to mammography. Chang et al identified a new electrochemical biosensor which could detect let-7a and miRNA-21 using functionalized metalorganic frameworks prepared by using porous UIO-66-NH 2 , and double stranded DNA.…”

Section: Ecs Sensors Plus 2022 1 021602mentioning

confidence: 61%

“…25 On this basis, numerous research has been reported for the single miRNA detection using varied electrochemical biosensors where electrochemistry technique reduces the false positive results due to interference and ambiguous signals to a greater extent, reveals its highly useful nature for physiological and pathological diagnosis over the conventionally used Real-time PCR (qRT-PCR) qRT-PCR. [26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43] The qRT-PCR even though is widely used powerful tool for miRNA assay and DCIS screening tool that could achieve high sensitivity involves tedious procedures and expensive primer sequences that restricts its further critical applications 44 (see Scheme 2).…”

Section: Dcis Stages Grades and Incidencementioning

confidence: 99%

Review—Systematic Review on Electrochemical Biosensing of Breast Cancer miRNAs to Develop Alternative DCIS Diagnostic Tool

Guruswami

Ponpandian

et al. 2022

ECS Sens. Plus

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Till date, no systematic review is conducted on electrochemical biosensing of multiplexed breast cancer miRNAs to identify their suitability as an alternative diagnostic tool for ductal carcinoma in-situ (DCIS). Original articles published in English from PUBMED, Science Direct, Scopus, MEDLINE, Cochrane Library, National Centre for Biotechnology Information, and Google scholar during Jan 2012– Feb 2022 were searched using set inclusion criteria. The Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) 2020 guidelines was followed to report this review. Methodological quality of the included studies was evaluated using Risk of Bias (ROB) assessment criteria. Out of 1973 screened articles, 17 studies were eligible and included in this review wherein, 1 study (5.88%) involving generic neutravidin modified nanolabel probe using biotintylated molecular beacons immobilized metal nanoparticles prepared using one pot assay to detect miRNAs 21 and 141 had low ROB and 16 studies (94.12%) had medium ROB. Medium ROB of majority of the included studies reveals its limited evidence to conclude its suitability for diagnosing DCIS using miRNAs. High quality studies with inter assays and validation are extensively needed for the development of diagnostic tool for DCIS via miRNAs.

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“…The proposed assay strategy reveals excellent analytical performance, and the biosensor can perform the analysis at the femtomolar level (1.2 fM) for miRNA-21 and with a LOD of 0.14 U/mL for CA 15-3. The biosensor provides not only a fast analytical response time, but also simplicity in preparation, high selectivity, reproducibility, and acceptable stability [ 51 ].…”

Section: Outcomementioning

confidence: 99%

Role of Nano-miRNAs in Diagnostics and Therapeutics

Coradduzza

Bellu

Congiargiu

et al. 2022

IJMS

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MicroRNAs (miRNA) are key regulators of gene expression, controlling different biological processes such as cellular development, differentiation, proliferation, metabolism, and apoptosis. The relationships between miRNA expression and the onset and progression of different diseases, such as tumours, cardiovascular and rheumatic diseases, and neurological disorders, are well known. A nanotechnology-based approach could match miRNA delivery and detection to move beyond the proof-of-concept stage. Different kinds of nanotechnologies can have a major impact on the diagnosis and treatment of miRNA-related diseases such as cancer. Developing novel methodologies aimed at clinical practice represents a big challenge for the early diagnosis of specific diseases. Within this context, nanotechnology represents a wide emerging area at the forefront of research over the last two decades, whose potential has yet to be fully attained. Nanomedicine, derived from nanotechnology, can exploit the unique properties of nanometer-sized particles for diagnostic and therapeutic purposes. Through nanomedicine, specific treatment to counteract only cancer-cell proliferation will be improved, while leaving healthy cells intact. In this review, we dissect the properties of different nanocarriers and their roles in the early detection and treatment of cancer.

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A gold nanoparticle-dye/poly(3-aminobenzylamine)/two dimensional MoSe2/graphene oxide electrode towards label-free electrochemical biosensor for simultaneous dual-mode detection of cancer antigen 15-3 and microRNA-21

Cited by 59 publications

References 59 publications

Highly Sensitive Detection of Carcinoembryonic Antigen via an Electrochemical Platform Fabricated by AuNPs/Streptavidin/Reduced Graphene Oxide

Highly Sensitive Detection of Carcinoembryonic Antigen via an Electrochemical Platform Fabricated by AuNPs/Streptavidin/Reduced Graphene Oxide

Review—Systematic Review on Electrochemical Biosensing of Breast Cancer miRNAs to Develop Alternative DCIS Diagnostic Tool

Role of Nano-miRNAs in Diagnostics and Therapeutics

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