A graphene-based label-free voltammetric immunosensor for sensitive detection of the egg allergen ovalbumin

Eissa, Shimaa; L’Hocine, Lamia; Siaj, Mohamed; Zourob, Mohammed

doi:10.1039/c3an36883a

Cited by 89 publications

(69 citation statements)

References 51 publications

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“…They are classified according to the type of used bioreceptor. The bioreceptors most commonly used are specific antibodies [93][94][95][96][97][98][99][100][101][102][103][104], single-stranded DNA molecules [105][106][107][108], aptamers [109][110][111][112], and other affinity receptors like lectins [113] and peptides [114]. The affinity reaction between these bioreceptors, which are immobilized onto electrodes or solid supports like magnetic beads (MBs), and the target allergen has been monitored using (chrono)amperometry [68,94,97,99,[101][102][103][104]109], voltammetry [93,95,96,98,102,103,106,107,[112][113][114] and electrochemical impedance spectroscopy [105,110,111].…”

Section: Electrochemical Affinity Biosensors For Allergensmentioning

confidence: 99%

“…Monitoring of the antigen-antibody interaction using DPV in the presence of [Fe(CN) 6 ] 4−/3− , allowed a LOD of 5 × 10 −8 g·mL −1 to be achieved. Immunosensors for β-lactoglobulin [94] and OVA [96] were developed by electro-grafting an aryl diazonium salt organic film on graphene-modified SPCEs. The capture antibody was covalently immobilized using EDC/NHS chemistry or glutaraldehyde on the carboxyphenyl [96] or nitrophenyl [95] groups of the film grafted onto the graphene electrodes by electrochemical reduction of the in situ generated diazonium salt.…”

Section: Electrochemical Affinity Biosensors For Allergensmentioning

confidence: 99%

“…Immunosensors for β-lactoglobulin [94] and OVA [96] were developed by electro-grafting an aryl diazonium salt organic film on graphene-modified SPCEs. The capture antibody was covalently immobilized using EDC/NHS chemistry or glutaraldehyde on the carboxyphenyl [96] or nitrophenyl [95] groups of the film grafted onto the graphene electrodes by electrochemical reduction of the in situ generated diazonium salt. The decrease in the [Fe(CN) 6 ] 3−/4− reduction peak current, measured by DPV after the immunological reaction, was used for the target allergen quantification.…”

Section: Electrochemical Affinity Biosensors For Allergensmentioning

confidence: 99%

“…In addition, given the sensitivity required for some allergens detection, many biosensor devices with improved sensitivity based on the use of nanomaterials, such as QDs [115], AuNPs [93,100,101,[106][107][108], MWCNTs [93] and graphene [95,96,106] have been developed. Worth to mention also the increasing tendency to fabricate these electrochemical biosensors onto screen-printed electrodes (SPEs) [85,[95][96][97][98][99][100][101][102][103][104]107,109,112,113] since they are suitable to be fabricated with different materials, to be drawn in diverse geometries and multiplexed formats and Recently, Sugawara et al [116] developed an electrochemical biosensor to detect ovalbumin (OVA) by adsorbing a specific peptide probe (peptide-1) conjugated with the electroactive daunomycin on a GCE.…”

Section: Electrochemical Affinity Biosensors For Allergensmentioning

confidence: 99%

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Electrochemical Affinity Biosensors in Food Safety

2017

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Safety and quality are key issues of today's food industry. Since the food chain is becoming more and more complex, powerful analytical methods are required to verify the performance of food safety and quality systems. Indeed, such methods require high sensitivity, selectivity, ability for rapid implementation and capability of automatic screening. Electroanalytical chemistry has, for decades, played a relevant role in food safety and quality assessment, taking more and more significance over time in the solution of analytical problems. At present, the implementation of electrochemical methods in the food is evident. This is in a large part due to the relevant results obtained by combining the attractive advantages of electrochemical transduction strategies (in terms of relatively simple hardware, versatility, interface with automatic logging and feasibility of application outside the laboratory environment) with those from biosensors technology. Important examples of enzyme electrochemical biosensors are those dedicated to the determination of glucose, alcohol or cholesterol are important examples. In addition, other types of different electrochemical biosensing approaches have emerged strongly in the last years. Among these, the strategies involving affinity interactions have been shown to possess a large number of applications. Therefore, electrochemical immunosensors and DNA-based biosensors have been widely used to determine major and minor components in foodstuffs, providing sufficient data to evaluate food freshness, the quality of raw materials, or the origin of samples, as well as to determine a variety of compounds at trace levels related to food safety such as micotoxins, allergens, drugs residues or pathogen microorganisms. This review discusses some critical examples of the latest advances in this area, pointing out relevant methodologies related to the measurement techniques, including the use of nanostructured electrodes and strategies for signal amplification.

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Section: Electrochemical Affinity Biosensors For Allergensmentioning

confidence: 99%

Section: Electrochemical Affinity Biosensors For Allergensmentioning

confidence: 99%

Section: Electrochemical Affinity Biosensors For Allergensmentioning

confidence: 99%

Section: Electrochemical Affinity Biosensors For Allergensmentioning

confidence: 99%

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Electrochemical Affinity Biosensors in Food Safety

2017

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“…23 Furthermore, a voltammetric immunosensor using a graphene-modified screen-printed carbon electrode was fabricated to sensitively determine OVA. 24 We proposed the sensing of α-phosvitin using a carbon paste electrode modified with the gallium(III)-acetylacetonate (AA) complex. 25 This procedure depended on an interaction between the phosphate groups and gallium(III) ions.…”

Section: Introductionmentioning

confidence: 99%

Construction of an Electrode Modified with Gallium(III) for Voltammetric Detection of Ovalbumin

et al. 2014

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Electrodes modified with gallium(III) complexes were constructed to detect ovalbumin (OVA). For immobilization of a gallium(III)-nitrilotriacetate (NTA) complex, the electrode was first covered with collagen film. After the amino groups of the film had reacted with isothiocyanobenzyl-NTA, the gallium(III) was then able to combine with the NTA moieties. Another design featured an electrode cast with a gallium(III)-acetylacetonate (AA) complex. The amount of gallium(III) in the NTA complex was equivalent to one-quarter of the gallium(III) that could be utilized from an AA complex. However, the calibration curves of OVA using gallium(III)-NTA and gallium(III)-AA complexes were linear in the ranges of 7.0 × 10 -11 -3.0 × 10 -9 M and 5.0 × 10 -10 -8.0 × 10 -9 M, respectively. The gallium(III) on the electrode with NTA complex had high flexibility due to the existence of a spacer between the NTA and the collagen film, and, therefore, the reactivity of the gallium(III) to OVA was superior to that of the gallium(III)-AA complex with no spacer.

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