A highly sensitive fluorimetric protocol based on isoindole formation for determination of gabapentin

Abstract

A new, simple, highly sensitive and selective spectrofluorimetric method was developed for determination of gabapentin through its derivatization with o-phthalaldehyde in the presence of 2-mercaptoethanol.

Green innovative fluorescence approach for the feasible and reliable assay of thiol-containing drugs; captopril as a model

“… 54 Therefore, the chain should be of reasonable length. In our laboratory, various compounds that contain aliphatic primary amino group such as gabapentin, 48 3-isobutyl gamma aminobutyric acid, 49 milnacipran, 50 and AMH 51 were analyzed through the isoindole formation reaction. However, the use of AMH was found to fulfil the aforementioned criteria of being has a side chain of appropriate length (C6) with two points of branching, one of them is close to the amino group.…”

“…The interaction of OPA with thiols, specially 2-ME and compounds containing primary amino group, was extensively used to determine primary amine compounds, [47][48][49][50][51] including pharmaceutical compounds. 52,53 Because of the essential presence of the thiol compound in the reaction vessel, the reaction could be possibly employed for the thiol-containing compound.…”

A new approach based on isoindole formation reaction for sensitive fluorimetric assay of milnacipran in tablets and biological fluids (plasma/urine)

“…The reaction proceeded in basic pH and 2-mercaptoethanol is essential for isoindole product stability. 20 The MLC derivative was monitored at (l ex 338.5 nm, l em 433.5 nm) ( Fig. 1) and (Scheme 1) elucidate isoindole formation mechanism.…”

Protonation of gabapentin: Ion mobility spectrometry and computational study

“…So, other methods like spectrofluorimetry and spectrophotometry have been applied to determination gabapentin. 30–35 It is found that the measurement of gabapentin in human matrices is complicated and laborious work, including extraction, precipitation, and derivatizations. Ion mobility spectroscopy (IMS), as a fast, sensitive, and low-cost technique, has been raised to measure drugs as solid and aqueous samples.…”