A Histochemical Method for the Simultaneous Demonstration of Capillaries and Fiber Type in Skeletal Muscle

Jd, Rosenblatt; Kuzon, WM; Mj, Plyley; Br, Pynn; Nh, McKee

doi:10.3109/10520298709107973

Cited by 77 publications

(71 citation statements)

References 24 publications

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“…Our results may be limited in that the stain used to simultaneously detect ®bre composition and capillaries 19 does not differentiate type 2 ®bre subtypes. There might have been a change in type 2 ®bre subtypes but this could not be measured with the methods used.…”

Section: Discussionmentioning

confidence: 89%

“…Sections were cut at a thickness of 10 mm at 7208C using a Tissue-Tek Cryostat (Miles Laboratories) and mounted on glass slides with a coverslip. Muscle sections were simultaneously stained for capillaries and ®bre types using the technique of Rosenblatt et al 19 as modi®ed for human muscle by Hepple et al 13 This technique does not allow for dierentiation of type 2 ®bre subtypes. Staining for each individual's pre-and post-training muscle biopsy were done within the same assay to decrease inter-assay variability.…”

Section: Muscle Biopsiesmentioning

confidence: 99%

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Histochemical changes in muscle of individuals with spinal cord injury following functional electrical stimulated exercise training

et al. 1999

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Study Design: Longitudinal training. Objectives: To determine the eects of functional electrical stimulated (FES) leg cycle ergometer training on muscle histochemical characteristics in individuals with motor-complete spinal cord injury (SCI). Setting: University of Alberta, Edmonton, Alberta, Canada. Methods: Six individuals with motor-complete SCI (age 31 ± 50 years; 3 ± 25 years postinjury) trained using FES leg cycle ergometry for 30 min, 3 days per week for 8 weeks. Biopsies of the vastus lateralis muscle were obtained pre-and post-training and analyzed for ®bre composition, ®bre size and capillarization. Results: The majority of muscle ®bres were classi®ed as type 2 pre-and post-training. Average ®bre area increased 23% (P50.05) and capillary number increased 39% (P50.05) with training. As a result of these proportional increases, capillarization expressed relative to ®bre area was unchanged with training. Conclusions: FES leg cycle ergometer training results in proportional increases in ®bre area and capillary number in individuals with SCI. Sponsorship: Supported by the Glenrose Rehabilitation Hospital.

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Section: Discussionmentioning

confidence: 89%

Section: Muscle Biopsiesmentioning

confidence: 99%

Histochemical changes in muscle of individuals with spinal cord injury following functional electrical stimulated exercise training

et al. 1999

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“…Capillary imaging was carried out by a modified ATPase staining method, 13 and visualized by light microscopy. Capillaries and muscle fibers from 3 random areas from each of 3 sections from each muscle were counted (Scion Image software).…”

Section: Capillary Densitymentioning

confidence: 99%

Diet-Induced Obesity in Female Mice Leads to Offspring Hyperphagia, Adiposity, Hypertension, and Insulin Resistance

et al. 2008

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Abstract-Maternal obesity is increasingly prevalent and may affect the long-term health of the child. We investigated the effects of maternal diet-induced obesity in mice on offspring metabolic and cardiovascular function. Female C57BL/6J mice were fed either a standard chow (3% fat, 7% sugar) or a palatable obesogenic diet (16% fat, 33% sugar) for 6 weeks before mating and throughout pregnancy and lactation. Offspring of control (OC) and obese dams (OO) were weaned onto standard chow and studied at 3 and 6 months of age. OO were hyperphagic from 4 to 6 weeks of age compared with OC and at 3 months locomotor activity was reduced and adiposity increased (abdominal fat pad mass; PϽ0.01). OO were heavier than OC at 6 months (body weight, PϽ0.05). OO abdominal obesity was associated with adipocyte hypertrophy and altered mRNA expression of ␤-adrenoceptor 2 and 3, 11␤HSD-1, and PPAR-␥ 2. OO showed resistance artery endothelial dysfunction at 3 months, and were hypertensive, as assessed by radiotelemetry (nighttime systolic blood pressure at 6 months [ Key Words: obesity Ⅲ pregnancy Ⅲ developmental programming Ⅲ metabolic syndrome Ⅲ appetite Ⅲ blood pressure Ⅲ mouse O besity among women of reproductive age is presenting a critical challenge to health care. 29% of USA women aged 20 to 39 years are reported to be clinically obese 1 and there is serious concern in many European countries over the increasing obesity among young women. 2 While obesity is associated with increased risk of almost every common complication of pregnancy, obesity in the mother may play a direct role in transmission of an obesogenic and diabetogenic trait from generation to generation. Increasing evidence suggests that children born of pregnancies complicated by either obesity or related gestational diabetes mellitus (GDM) are at increased risk of obesity, impaired glucose tolerance, and other facets of the metabolic syndrome. 3 Animal models have proven invaluable in interrogation of associations between maternal diet and body composition and offspring phenotype. 4 Those studies which have addressed effects of maternal calorific excess, including several from our laboratory, have generally fed rats diets rich in animal fat. 4 -7 Because young women of reproductive age often consume excessive amounts of sugars as well as fats, 8 the relevance of a diet rich in fat alone is limited. In this study, we induced obesity by feeding mice a highly palatable diet rich in sugars and animal fat, and addressed the hypothesis that diet-induced obesity during pregnancy can transmit a propensity for adiposity, glucose intolerance, and cardiovascular dysfunction to the offspring. Obesity was induced in female mice and offspring cardiovascular and metabolic function

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“…Opposite results were found in the myotomal muscle of M. furnieri, C. guatucupa and Engraulis anchoita Hubbs & Marini, 1935(Devincenti et al 2000a, b, 2015. In mammals, prolonged fixation stained the capillaries and, in turn, the reaction was more intense in type II (rapid) fibers than in type I (slow) fibers (rOsenBlatt et al 1987). In bird muscles, however, this technique permitted the detection of capillaries only (FOuces et al 1993).…”

Section: 10 11mentioning

confidence: 99%

The adductor pectoral fin muscle of Micropogonias furnieri (Perciformes: Sciaenidae): a morphological and histochemical study

Hernández¹,

Longo²,

Devincenti³

et al. 2016

Zoologia (Curitiba)

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ABSTRACT. The adductor pectoral fin muscle in post-spawning females of the white croaker Micropogonias furnieri (Desmarest, 1823) is described from a morphological, histochemical and morphometric perspectives. A description of the morphological characteristics was conducted after dissections in different layers, down to the deep layer. Five muscles were found: superficial, medial, radial and deep adductors, and dorsal arrector. Their fibers were studied after applying histochemical techniques: succinic dehydrogenase (SDH) to reveal mitochondria, periodic acid-Schiff (PAS) for glycogen, Sudan black for lipids, myosin adenosinetriphosphatase (mATPase) to reveal the types of fibers, and modified mATPase to evidence the capillaries. Fiber diameters were measured and the number of capillaries was counted. Fiber subtypes named small, medium and large were found within red, pink and white fibers, the latter prevailing. Staining homogeneity was observed in white fibers after alkaline pre-incubations. The number of capillaries decreased from red to white fibers. Due to the prevalence of white fibers, the adductor muscle of the pectoral fins appears to be capable of rapid and discontinuous movements, which are important to body stabilization during subcarangiform swimming. The homogenous staining of white fibers observed in this research corresponds to the post-spawning gonad stage studied.

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A Histochemical Method for the Simultaneous Demonstration of Capillaries and Fiber Type in Skeletal Muscle

Cited by 77 publications

References 24 publications

Histochemical changes in muscle of individuals with spinal cord injury following functional electrical stimulated exercise training

Histochemical changes in muscle of individuals with spinal cord injury following functional electrical stimulated exercise training

Diet-Induced Obesity in Female Mice Leads to Offspring Hyperphagia, Adiposity, Hypertension, and Insulin Resistance

The adductor pectoral fin muscle of Micropogonias furnieri (Perciformes: Sciaenidae): a morphological and histochemical study

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