A histological study of the effect of developmental stage of tooth-germ on transplantation to hamster cheek pouch

Al-Talabani, Nazar G.; Smith, Colin J.

doi:10.1016/0003-9969(79)90220-6

Cited by 8 publications

(1 citation statement)

References 13 publications

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“…Actually, many parts of mouse body have been tested for hosting grafted tooth germs. These sites include the anterior eye chamber (Kollar and Fisher,1980; Yoshikawa and Kollar,1981; Lumsden and Buchanan,1986), renal subcapsular site (Bartlett and Reade,1973), subcutaneous tissue (Ivanyi,1965), cheek pouch (Al‐Talabani and Smith,1979), and spleen (Ishizeki et al,1987). In addition, chick embryonic wing buds were also shown to be an effective in vivo culture system for tooth development (Koyama et al,2002).…”

Section: Introductionmentioning

confidence: 99%

Mouse embryonic diastema region is an ideal site for the development of ectopically transplanted tooth germ

Song¹,

Yan²,

Muneoka³

2008

Developmental Dynamics

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The anterior eye chamber and the kidney capsule of the mouse have been traditionally used for long-term culture of tooth germ grafts. However, although these sites provide an excellent growth environment, they do not represent real in situ sites for the development of a grafted tooth germ. Here, we describe a protocol to transplant a tooth germ into the mandibular diastema region of mouse embryos using exo utero surgery. Our results demonstrate that the mouse embryonic diastema region represents a normal physiological environment for the development of transplanted tooth germs. Transplanted tooth germs developed synchronically with and became indistinguishable from the endogenous ones. These ectopic teeth were vascularized and surrounded with nerve fibers, and were able to erupt normally. Thus, the exo utero transplantation approach will provide a new avenue to study tooth development and regeneration. Developmental Dynamics 237:411-416, 2008.

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Section: Introductionmentioning

confidence: 99%

Mouse embryonic diastema region is an ideal site for the development of ectopically transplanted tooth germ

Song¹,

Yan²,

Muneoka³

2008

Developmental Dynamics

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Chick limbs with mouse teeth: An effective in vivo culture system for tooth germ development and analysis

Koyama

Shimo

et al. 2002

Developmental Dynamics

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Mouse tooth germ development is currently studied by three main approaches: in wild-type and mutant mouse lines, after transplantation of tooth germs to ectopic sites, and in organ culture. The in vivo approaches are the most physiological but do not provide accessibility to tooth germs for further experimental manipulation. Organ cultures, although readily accessible, do not sustain full tooth germ development and are appropriate for short-term analysis. Thus, we sought to establish a new approach that would combine experimental accessibility with sustained development. We implanted fragments of embryonic day 12 mouse embryo first branchial arch containing early bud stage tooth germs into the lateral mesenchyme of day 4 -5 chick embryo wing buds in ovo. Eggs were reincubated, and implanted tissues were examined by histochemistry and in situ hybridization over time. The tooth germs underwent seemingly normal growth, differentiation, and morphogenesis. They reached the cap, bell, and crown stages in approximately 3, 6, and 10 days, respectively, mimicking in a striking manner native temporal patterns. To examine mechanisms regulating tooth germ development, we first implanted tooth germ fragments, microinjected them with neutralizing antibodies to the key signaling molecule Sonic hedgehog (Shh), and examined them over time. Tooth germ development was markedly delayed, as revealed by poor morphogenesis and lack of mature ameloblasts and odontoblasts displaying characteristic traits such as an elongated cell shape, nuclear relocalization, and amelogenin gene expression. These phenotypic changes began to be reversed upon further incubation. The data show that the limb bud represents an effective, experimentally accessible as well as economical system for growth and analysis of developing tooth germs. The inhibitory effects of Shh neutralizing antibody treatment are discussed in relation to roles of this signaling pathway proposed by this and other groups previously. Developmental Dynamics 226:149 -154, 2003.

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Development and cell fate in interspecific (mus musculus/mus caroli) intraocular transplants of mouse molar tooth-germ tissues detected by in situ hybridization

Lubbock

Harrison

Lumsden

et al. 1996

Archives of Oral Biology

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A histological study of the effect of developmental stage of tooth-germ on transplantation to hamster cheek pouch

Cited by 8 publications

References 13 publications

Mouse embryonic diastema region is an ideal site for the development of ectopically transplanted tooth germ

Mouse embryonic diastema region is an ideal site for the development of ectopically transplanted tooth germ

Chick limbs with mouse teeth: An effective in vivo culture system for tooth germ development and analysis

Development and cell fate in interspecific (mus musculus/mus caroli) intraocular transplants of mouse molar tooth-germ tissues detected by in situ hybridization

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