A homologue of the breast cancer-associated gene BARD1 is involved in DNA repair in plants

Reidt, Wim; Wurz, Rebecca; Wanieck, Kristina; Chu, Ha Hoang; Puchta, Holger

doi:10.1038/sj.emboj.7601313

Cited by 49 publications

(75 citation statements)

References 62 publications

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“…A mutation in the BRCA1-associated RING domain1 gene caused both a defect in DNA repair and in the maintenance of SAM organization by repressing WUS expression in plants (Reidt et al, 2006;Han et al, 2008). In a previous study, double-knockdown lines of the cyclin-dependent kinases CDKB2;1 and CDKB2;2 showed abnormal SAMs and phyllotaxis, suggesting a close link between cell cycle progression and the regulation of meristem organization in plants (Andersen et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

A DNA2 Homolog Is Required for DNA Damage Repair, Cell Cycle Regulation, and Meristem Maintenance in Plants

2016

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Plant meristem cells divide and differentiate in a spatially and temporally regulated manner, ultimately giving rise to organs. In this study, we isolated the Arabidopsis jing he sheng 1 (jhs1) mutant, which exhibited retarded growth, an abnormal pattern of meristem cell division and differentiation, and morphological defects such as fasciation, an irregular arrangement of siliques, and short roots. We identified JHS1 as a homolog of human and yeast DNA Replication Helicase/Nuclease2, which is known to be involved in DNA replication and damage repair. JHS1 is strongly expressed in the meristem of Arabidopsis. The jhs1 mutant was sensitive to DNA damage stress and had an increased DNA damage response, including increased expression of genes involved in DNA damage repair and cell cycle regulation, and a higher frequency of homologous recombination. In the meristem of the mutant plants, cell cycle progression was delayed at the G2 or late S phase and genes essential for meristem maintenance were misregulated. These results suggest that JHS1 plays an important role in DNA replication and damage repair, meristem maintenance, and development in plants.

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Section: Discussionmentioning

confidence: 99%

A DNA2 Homolog Is Required for DNA Damage Repair, Cell Cycle Regulation, and Meristem Maintenance in Plants

2016

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“…involved in HR or its regulation (e.g. bard1, rad51C, and rad54) are also sensitive to cross-linking agents (Abe et al, 2005;Osakabe et al, 2006;Reidt et al, 2006;Shaked et al, 2006). Moreover, they are deficient in intrachromosomal HR, but in contrast to rad5a not only after bleomycin treatment but also without DSB induction.…”

Section: Atrad5 Is Involved In Dsb-induced Hr In Somatic Cellsmentioning

confidence: 99%

A Homolog ofScRAD5Is Involved in DNA Repair and Homologous Recombination in Arabidopsis

et al. 2008

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Rad5 is the key component in the Rad5-dependent error-free branch of postreplication repair in yeast (Saccharomyces cerevisiae). Rad5 is a member of the Snf2 ATPase/helicase family, possessing as a characteristic feature, a RING-finger domain embedded in the Snf2-helicase domain and a HIRAN domain. Yeast mutants are sensitive to DNA-damaging agents and reveal differences in homologous recombination. By sequence comparisons we were able to identify two homologs (AtRAD5a and AtRAD5b) in the Arabidopsis thaliana genome, sharing about 30% identity and 45% similarity to yeast Rad5. AtRad5a and AtRad5b have the same kind of domain organization with a higher degree of similarity to each other than to ScRad5. Surprisingly, both genes differ in function: whereas two independent mutants of Atrad5a are hypersensitive to the cross-linking agents mitomycin C and cis-platin and to a lesser extent to the methylating agent, methyl methane sulfonate, the Atrad5b mutants did not exhibit any sensitivity to all DNA-damaging agents tested. An Atrad5a/Atrad5b double mutant resembles the sensitivity phenotype of the Atrad5a single mutants. Moreover, in contrast to Atrad5b, the two Atrad5a mutants are deficient in homologous recombination after treatment with the double-strand break-inducing agent bleomycin. Our results suggest that the RAD5-dependent error-free branch of postreplication repair is conserved between yeast and plants, and that AtRad5a might be functionally homologous to ScRad5.

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“…BRCA1 and BRCA2 orthologs appear to be present in all vertebrate genomes. A BRCA2 homolog has been identified in the fungus Ustilago maydis [20] and homologs of BRCA1 exist in worms and in some plant species [21][22][23][24], but both genes appear to be absent from the genomes of Saccharomyces cerevisiae and Schizosaccharomyces pombe. The reason for this patchy representation of BRCA genes across evolution is not well understood.…”

Section: Introductionmentioning

confidence: 99%

Minding the gap: The underground functions of BRCA1 and BRCA2 at stalled replication forks

Nagaraju

Scully

2007

DNA Repair

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The hereditary breast and ovarian cancer predisposition genes, BRCA1 and BRCA2, participate in the repair of DNA double strand breaks by homologous recombination. Circumstantial evidence implicates these genes in recombinational responses to DNA polymerase stalling during the S phase of the cell cycle. These responses play a key role in preventing genomic instability and cancer. Here, we review the current literature implicating the BRCA pathway in HR at stalled replication forks and explore the hypothesis that BRCA1 and BRCA2 participate in the recombinational resolution of single stranded DNA lesions termed "daughter strand gaps", generated during replication across a damaged DNA template.

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A homologue of the breast cancer-associated gene BARD1 is involved in DNA repair in plants

Cited by 49 publications

References 62 publications

A DNA2 Homolog Is Required for DNA Damage Repair, Cell Cycle Regulation, and Meristem Maintenance in Plants

A DNA2 Homolog Is Required for DNA Damage Repair, Cell Cycle Regulation, and Meristem Maintenance in Plants

A Homolog ofScRAD5Is Involved in DNA Repair and Homologous Recombination in Arabidopsis

Minding the gap: The underground functions of BRCA1 and BRCA2 at stalled replication forks

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