A hydrogen-evolving enzyme is present in Frankia sp. R43

Mohapatra, Anasuya; Leul, Melakeselam; Mattsson, Ulrika; Sellstedt, Anita

doi:10.1111/j.1574-6968.2004.tb09652.x

Cited by 6 publications

(3 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various industrial processes have been developed that use these natural systems and attempt to mimic their capacity for hydrogen production. One strain of Frankia, designated R43, was demonstrated to produce large quantities of hydrogen under anaerobic conditions using the carbon source propionate [20]. It was subsequently proven that its hydrogen-producing enzyme is present in both its hyphae and its vesicles [20].…”

Section: Heterotrophic Production Of Hydrogenmentioning

confidence: 99%

Biomass‐Based Energy Production

Arshadi

Sellstedt

2014

Introduction to Chemicals From Biomass

Self Cite

View full text Add to dashboard Cite

Section: Heterotrophic Production Of Hydrogenmentioning

confidence: 99%

Biomass‐Based Energy Production

Arshadi

Sellstedt

2014

Introduction to Chemicals From Biomass

Self Cite

View full text Add to dashboard Cite

“…Processes with industrial applications have adopted natural systems and tried to mimic these for hydrogen production. One strain of Frankia, designated R43, was shown to produce a large quantity of hydrogen in anaerobic conditions using the carbon source propionate (Mohapatra et al 2004). Figure 6.4 presents the different cells of Frankia R43, both in the hyphae and vesicles.…”

Section: Heterotrophic Production Of Hydrogenmentioning

confidence: 99%

“…Figure 6.4 presents the different cells of Frankia R43, both in the hyphae and vesicles. It was earlier shown that the hydrogen-producing enzyme is present both in hyphae and vesicles (Mohapatra et al, 2004). With regard to the need for substrates for industrial hydrogen production, there is a wide range of substrates produced in different industries that could be used.…”

Section: Heterotrophic Production Of Hydrogenmentioning

confidence: 99%

Production of Energy from Biomass

Arshadi

Sellstedt

2008

Introduction to Chemicals From Biomass

Self Cite

View full text Add to dashboard Cite

The transport sector generates a lot of emissions of different gases and particles in the air. The gases are released by combustion of different fossil fuels in car engines. These emissions have a negative effect on human health and are also now known to cause vast environmental changes. Several of these gases are greenhouse gases such as CO 2 , CH 4 , N 2 O, etc. There is very strong evidence of a correlation between greenhouse gases and worldwide weather changes resulting in global warming. These negative environmental effects together with increasing energy demands in the world and depletion of fossil fuels in the near future, in combination with longdistance transport have had a positive impact on developing new energy sources. Bioenergy (energy derived from biological sources) production from renewable resources has increased during recent decades. World energy demand has dramatically increased and new sources of energy, beside fossil resources, are needed. The European Union is prompting its member states to increase their use of biofuels to meet a target share of 5.75% of the total fuel market by 2010. However, they had only reached an average share of 1.4% of the biofuel usage for all member states in 2005. To meet the EU target it is necessary to act in several different areas and develop several different bioenergy products, depending on regional possibilities Introduction to Chemicals from Biomass Edited by James Clark and Fabien Deswarte

show abstract

Structural and gene expression analyses of uptake hydrogenases and other proteins involved in nitrogenase protection in Frankia

et al. 2013

View full text Add to dashboard Cite

The actinorhizal bacterium Frankia expresses nitrogenase and can therefore convert molecular nitrogen into ammonia and the by-product hydrogen. However, nitrogenase is inhibited by oxygen. Consequently, Frankia and its actinorhizal hosts have developed various mechanisms for excluding oxygen from their nitrogen-containing compartments. These include the expression of oxygen-scavenging uptake hydrogenases, the formation of hopanoid-rich vesicles, enclosed by multi-layered hopanoid structures, the lignification of hyphal cell walls, and the production of haemoglobins in the symbiotic nodule. In this work, we analysed the expression and structure of the so-called uptake hydrogenase (Hup), which catalyses the in vivo dissociation of hydrogen to recycle the energy locked up in this 'waste' product. Two uptake hydrogenase syntons have been identified in Frankia: synton 1 is expressed under freeliving conditions while synton 2 is expressed during symbiosis. We used qPCR to determine synton 1 hup gene expression in two Frankia strains under aerobic and anaerobic conditions. We also predicted the 3D structures of the Hup protein subunits based on multiple sequence alignments and remote homology modelling. Finally, we performed BLAST searches of genome and protein databases to identify genes that may contribute to the protection of nitrogenase against oxygen in the two Frankia strains. Our results show that in Frankia strain ACN14a, the expression patterns of the large (HupL1) and small (HupS1) uptake hydrogenase subunits depend on the abundance of oxygen in the external environment. Structural models of the membrane-bound hydrogenase subunits of ACN14a showed that both subunits resemble the structures of known [NiFe] hydrogenases (Volbeda et al. 1995), but contain fewer cysteine residues than the uptake hydrogenase of the Frankia DC12 and Eu1c strains. Moreover, we show that all of the investigated Frankia strains have two squalene hopane cyclase genes (shc1 and shc2). The only exceptions were CcI3 and the symbiont of Datisca glomerata, which possess shc1 but not shc2. Four truncated haemoglobin genes were identified in Frankia ACN14a and Eu1f, three in CcI3, two in EANpec1 and one in the Datisca glomerata symbiont (Dg).

show abstract

A hydrogen-evolving enzyme is present in Frankia sp. R43

Cited by 6 publications

References 38 publications

Biomass‐Based Energy Production

Biomass‐Based Energy Production

Production of Energy from Biomass

Structural and gene expression analyses of uptake hydrogenases and other proteins involved in nitrogenase protection in Frankia

Contact Info

Product

Resources

About