A kinesin Klp10A mediates cell cycle-dependent shuttling of Piwi between nucleus and nuage

Venkei, Zsolt; Choi, Charlotte P; Feng, Suhua; Chen, Cuie; Jacobsen, Steven E.; Kim, John K.; Yamashita, Yukiko

doi:10.1371/journal.pgen.1008648

Cited by 7 publications

(3 citation statements)

References 91 publications

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“…Mechanistically, our findings support an association between PIWIL1 and the mitotic spindle, as previously observed in germ cells (Rodriguez et al, 2005; Venkei et al, 2020). Taken together, our results highlight the relevance of PIWI proteins in the regulation of the cell cycle in undifferentiated cells, possibly explaining their unexpected but widely documented expression in cancer.…”

Section: Introductionsupporting

confidence: 91%

PIWIL1 is recruited to Centrosomes during Mitosis in Colorectal Cancer Cells and is linked to cell cycle progression

Garcia-Silva,

Montenegro,

Dacosta

et al. 2024

Preprint

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PIWI proteins, traditionally associated with germline development, have recently gained attention for their expression in various cancers, including colorectal cancer (CRC). However, the molecular mechanisms underlying their reactivation and impact on cancer initiation and progression remain elusive. Here, we found that PIWIL1 is expressed at relatively high levels in CRC-derived samples and cell lines, where it undergoes a dynamic re-localization to the centrosome during mitosis. Knockdown of PIWIL1 induces G2/M arrest associated to disruption of mitotic spindle and aberrant metaphase events, highlighting its role in cell cycle progression. We have also found that expression of PIWIL1 is lost during differentiation of Caco-2 cells into enterocytes and that PIWIL1 is expressed in cells at the base of intestinal crypts in normal human colon tissue, where intestinal stem cells are known to reside. Thus, it is possible that the presence of PIWIL1 in cancer cells reflects a physiological role of this protein in stem cell maintenance, what would argue in favor of the proposed stem cell origin of CRC. Supporting this view, dedifferentiation of human fibroblasts into induced pluripotent stem cells (iPSc) implies reactivation of PIWIL2 expression, another member of the PIWI protein family. Overall, our findings suggest a role of PIWIL1 in mediating cell cycle dynamics, both in colorectal cancer cells and possibly also in intestinal stem cells. In a broader aspect, we provide support for an involvement of PIWI proteins in somatic stem cell maintenance, expanding nongonadal functions for this protein family.

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Section: Introductionsupporting

confidence: 91%

PIWIL1 is recruited to Centrosomes during Mitosis in Colorectal Cancer Cells and is linked to cell cycle progression

Garcia-Silva,

Montenegro,

Dacosta

et al. 2024

Preprint

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show abstract

“…Mechanistically, our ndings support an association between PIWIL1 and the mitotic spindle, as previously observed in germ cells [12], [13]. Taken together, our results highlight the relevance of PIWI proteins in the regulation of the cell cycle in undifferentiated cells, possibly explaining their unexpected but widely documented expression in cancer.…”

Section: Introductionsupporting

confidence: 88%

PIWIL1 is recruited to Centrosomes during Mitosis in Colorectal Cancer Cells and is linked to cell cycle progression

Garcia-Silva,

Montenegro,

Dacosta

et al. 2024

Preprint

View full text Add to dashboard Cite

Background PIWI proteins, traditionally associated with germline development, have recently gained attention for their expression in various cancers, including colorectal cancer. However, the molecular mechanisms underlying their reactivation and impact on cancer initiation and progression remain elusive.Results Here, we found that PIWIL1 is expressed at relatively high levels in CRC-derived samples and cell lines, where it undergoes a dynamic re-localization to the centrosome during mitosis. Knockdown of PIWIL1 induces G2/M arrest associated to disruption of mitotic spindle and aberrant metaphase events, highlighting its role in cell cycle progression. We have also found that expression of PIWIL1 is lost during differentiation of Caco-2 cells into enterocytes and that PIWIL1 is expressed in cells at the base of intestinal crypts in normal human colon tissue, where intestinal stem cells are known to reside. Thus, it is possible that the presence of PIWIL1 in cancer cells reflects a physiological role of this protein in stem cell maintenance, what would argue in favor of the proposed stem cell origin of CRC. Supporting this view, dedifferentiation of human fibroblasts into induced pluripotent stem cells (iPSc) implies reactivation of PIWIL2 expression, another member of the PIWI protein family.Conclusions Overall, our findings suggest a role of PIWIL1 in mediating cell cycle dynamics, both in colorectal cancer cells and possibly also in intestinal stem cells. In a broader aspect, we provide support for an involvement of PIWI proteins in somatic stem cell maintenance, expanding non-gonadal functions for this protein family.

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“…In D. melanogaster , the biogenesis of piRNA in germ cells is processed through the ping-pong cycle, mainly in a specific region in the cytoplasm called Nuage and partly in the outer membrane of mitochondria [ 59 ]. The transport of piRNA precursor transcripts in this process primarily depends on the exchange of two cytoplasmic PIWI proteins: Aubergine (Aub) and Argonaute 3 (Ago3) [ 60 – 62 ].…”

Section: Biosynthesis and Basic Functions Of Pirnasmentioning

confidence: 99%

Novel roles of PIWI proteins and PIWI-interacting RNAs in human health and diseases

Wu,

Yu,

Zhang

et al. 2023

Cell Commun Signal

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Non-coding RNA has aroused great research interest recently, they play a wide range of biological functions, such as regulating cell cycle, cell proliferation, and intracellular substance metabolism. Piwi-interacting RNAs (piRNAs) are emerging small non-coding RNAs that are 24–31 nucleotides in length. Previous studies on piRNAs were mainly limited to evaluating the binding to the PIWI protein family to play the biological role. However, recent studies have shed more lights on piRNA functions; aberrant piRNAs play unique roles in many human diseases, including diverse lethal cancers. Therefore, understanding the mechanism of piRNAs expression and the specific functional roles of piRNAs in human diseases is crucial for developing its clinical applications. Presently, research on piRNAs mainly focuses on their cancer-specific functions but lacks investigation of their expressions and epigenetic modifications. This review discusses piRNA’s biogenesis and functional roles and the recent progress of functions of piRNA/PIWI protein complexes in human diseases.

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A kinesin Klp10A mediates cell cycle-dependent shuttling of Piwi between nucleus and nuage

Cited by 7 publications

References 91 publications

PIWIL1 is recruited to Centrosomes during Mitosis in Colorectal Cancer Cells and is linked to cell cycle progression

PIWIL1 is recruited to Centrosomes during Mitosis in Colorectal Cancer Cells and is linked to cell cycle progression

PIWIL1 is recruited to Centrosomes during Mitosis in Colorectal Cancer Cells and is linked to cell cycle progression

Novel roles of PIWI proteins and PIWI-interacting RNAs in human health and diseases

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