“…A wealth of structural and functional information is available for both wild-type (wt) and mutant PBGDs, including several three-dimensional structures as well as kinetic, biophysical, and computational data ( Awan et al., 1997 ; Bung et al., 2019 ; Bustad et al., 2013 ; Hadener et al., 1999 ; Louie et al., 1992 ; Niemann et al., 1994 ; Pluta et al., 2018 ; Roberts et al., 2013 ; Shoolingin-Jordan et al., 1996 ; Song et al., 2009 ). The crystal structures of wt-PBGD have so far been solved for human (PDB: 3ECR , 5M7F ( Pluta et al., 2018 ; Song et al., 2009 )), Escherichia coli (PDB: 1PDA ( Louie et al., 1992 )), Arabidopsis thaliana (PDB: 4HTG ( Roberts et al., 2013 )), Bacillus megaterium (PDB: 4MLV ( Azim et al., 2014 )) and Vibrio cholera (PDB: 5H6O ( Uchida et al., 2018 )) enzymes, showing the same basic topology with three separate domains ( Figure 1 A).…”