A label-free affinity sensor with compensation of unspecific protein interaction by a highly sensitive integrated optical Mach–Zehnder interferometer on silicon

Brosinger, Franz; Freimuth, H.; Lacher, M.; Ehrfeld, W.; Gedig, Erk; Katerkamp, Andreas; Spener, Friedrich; Cammann, Karl

doi:10.1016/s0925-4005(97)00226-8

Cited by 87 publications

(29 citation statements)

References 6 publications

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“…In our sensor, LOD = 5 x 10 -7 RIU. These values for sensitivity and LOD show good performances of our sensors in comparison with other MZI platforms [16,17]. …”

Section: Sensing Measurementssupporting

confidence: 54%

Asymmetric Mach–Zehnder Interferometer Using Biosensors

2018

IJRTER

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We present a study of Aflatoxin M1 detection by photonic biosensors based on Si3N4 Asymmetric MachZehnder Interferometer (aMZI) functionalized with antibodies fragments (Fabʹ). We measured a best volumetric sensitivity of 10 4 rad/RIU, leading to a Limit of Detection below 5x10 -7 RIU. On sensors functionalized with Fabʹ, we performed specific and non-specific sensing measurements at various toxin concentrations. Reproducibility of the measurements and re-usability of the sensor were also investigated.

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“…In our sensor, LOD = 5 x 10 -7 RIU. These values for sensitivity and LOD show good performances of our sensors in comparison with other MZI platforms [16,17]. …”

Section: Sensing Measurementssupporting

confidence: 54%

Asymmetric Mach–Zehnder Interferometer Using Biosensors

2018

IJRTER

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“…Examples include on-chip embodiments of a Mach-Zehnder (11)(12)(13)(14) and Young Interferometer (15), dual polarization interferometer (16), porous Si (17) and nanoporous Al (18) sensors, diffraction optics technology (19), and the biological compact disk (BioCD) (20,21), as well as BSI (9,10,22). The Mach-Zehnder and Young dual polarization interferometers use wave guiding to monitor binding with surface interaction sensing, which requires relatively long sections of the channel to be coated with the target and large sample volumes to be introduced to facilitate micromolar to nanomolar detection limits.…”

mentioning

confidence: 99%

Free-Solution, Label-Free Molecular Interactions Studied by Back-Scattering Interferometry

Bornhop

Latham

Kussrow

et al. 2007

Science

191

216

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Free-solution, label-free molecular interactions were investigated with back-scattering interferometry in a simple optical train composed of a helium-neon laser, a microfluidic channel, and a position sensor. Molecular binding interactions between proteins, ions and protein, and small molecules and protein, were determined with high dynamic range dissociation constants (K d spanning six decades) and unmatched sensitivity (picomolar K d 's and detection limits of 10,000s of molecules). With this technique, equilibrium dissociation constants were quantified for protein A and immunoglobulin G, interleukin-2 with its monoclonal antibody, and calmodulin with calcium ion Ca 2+ , a small molecule inhibitor, the protein calcineurin, and the M13 peptide. The high sensitivity of back-scattering interferometry and small volumes of microfluidics allowed the entire calmodulin assay to be performed with 200 picomoles of solute.

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“…Apart from high cost, the only limitation associated with this technique is that the sensitivity depends on the molecular mass of the adsorbed layer, which in turn controls the optical thickness, so low concentrations of small molecules (molecular mass < 250, including most haptens) are unlikely to be measurable directly without additional labeling. In addition to SPR, Mach -Zehnder interferometers [377], RIFS [201], [202], and so-called grating couplers [378] are also used for optical immunosensing. An interesting step towards miniaturization was introduced by the development of practicable fiber optical SPR systems by JORGENSON AND YEE [379] and KATERKAMP et al in the ICB Muenster [380], [381].…”

Section: Affinity Sensors -Immuno-probesmentioning

confidence: 99%

Chemical and Biochemical Sensors

Cammann¹,

Roß²,

Katerkamp³

et al. 2001

Ullmann's Encyclopedia of Industrial Chemistry

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The article contains sections titled: 1. Introduction to the Field of Sensors and Actuators 2. Chemical Sensors 2.1. Introduction 2.2. Molecular Recognition Processes and Corresponding Selectivities 2.2.1. Catalytic Processes in Calorimetric Devices 2.2.2. Reactions at Semiconductor Surfaces and Interfaces Influencing Surface or Bulk Conductivities 2.2.3. Selective Ion Conductivities in Solid‐State Materials 2.2.4. Selective Adsorption ‐ Distribution and Supramolecular Chemistry at Interfaces 2.2.5. Selective Charge‐Transfer Processes at Ion‐Selective Electrodes (Potentiometry) 2.2.6. Selective Electrochemical Reactions at Working Electrodes (Voltammetry and Amperometry) 2.2.7. Molecular Recognition Processes Based on Molecular Biological Principles 2.3. Transducers for Molecular Recognition: Processes and Sensitivities 2.3.1. Electrochemical Sensors 2.3.1.1. Self‐Indicating Potentiometric Electrodes 2.3.1.2. Voltammetric and Amperometric Cells 2.3.1.3. Conductance Devices 2.3.1.4. Ion‐Selective Field‐Effect Transistors (ISFETs) 2.3.2. Optical Sensors 2.3.2.1. Fiber‐Optical Sensors 2.3.2.2. Integrated Optical Chemical and Biochemical Sensors 2.3.2.3. Surface Plasmon Resonance 2.3.2.4. Reflectometric Interference Spectroscopy 2.3.3. Mass‐Sensitive Devices 2.3.3.1. Introduction 2.3.3.2. Fundamental Principles and Basic Types of Transducers 2.3.3.3. Theoretical Background 2.3.3.4. Technical Considerations 2.3.3.5. Specific Applications 2.3.3.6. Conclusions and Outlook 2.3.4. Calorimetric Devices 2.4. Problems Associated with Chemical Sensors 2.5. Multisensor Arrays, Electronic Noses, and Tongues 3. Biochemical Sensors (Biosensors) 3.1. Definitions, General Construction, and Classification 3.2. Biocatalytic (Metabolic) Sensors 3.2.1. Monoenzyme Sensors 3.2.2. Multienzyme Sensors 3.2.3. Enzyme Sensors for Inhibitors ‐ Toxic Effect Sensors 3.2.4. Biosensors Utilizing Intact Biological Receptors 3.3. Affinity Sensors ‐ Immuno‐Probes 3.3.1. Direct‐Sensing Immuno‐Probes without Marker Molecules 3.3.2. Indirect‐Sensing Immuno‐Probes using Marker Molecules 3.4. Whole‐Cell Biosensors 3.5. Problems and Future Prospects 4. Actuators and Instrumentation 5. Future Trends and Outlook

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A label-free affinity sensor with compensation of unspecific protein interaction by a highly sensitive integrated optical Mach–Zehnder interferometer on silicon

Cited by 87 publications

References 6 publications

Asymmetric Mach–Zehnder Interferometer Using Biosensors

Asymmetric Mach–Zehnder Interferometer Using Biosensors

Free-Solution, Label-Free Molecular Interactions Studied by Back-Scattering Interferometry

Chemical and Biochemical Sensors

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