A Label-free CMOS DNA Microarray based on Charge Sensing

Anderson, Erik P.; Daniels, John R.; Heng, Yu; Lee, T.; Pourmand, Nader

doi:10.1109/imtc.2008.4547305

Cited by 10 publications

(8 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Yet another approach functions both as a hybridization and polymerization sensor, and could therefore be used to sequence DNA [140]. Probe DNA is immobilized over a polymer layer by spotting, and target DNA of a different length is then introduced.…”

Section: Biochemical Sensorsmentioning

confidence: 99%

The potential of microelectrode arrays and microelectronics for biomedical research and diagnostics

et al. 2010

View full text Add to dashboard Cite

Planar microelectrode arrays (MEAs) are devices that can be used in biomedical and basic in vitro research to provide extracellular electrophysiological information about biological systems at high spatial and temporal resolution. Complementary metal oxide semiconductor (CMOS) is a technology with which MEAs can be produced on a microscale featuring high spatial resolution and excellent signal-to-noise characteristics. CMOS MEAs are specialized for the analysis of complete electrogenic cellular networks at the cellular or subcellular level in dissociated cultures, organotypic cultures, and acute tissue slices; they can also function as biosensors to detect biochemical events. Models of disease or the response of cellular networks to pharmacological compounds can be studied in vitro, allowing one to investigate pathologies, such as cardiac arrhythmias, memory impairment due to Alzheimer's disease, or vision impairment caused by ganglion cell degeneration in the retina.

show abstract

Section: Biochemical Sensorsmentioning

confidence: 99%

The potential of microelectrode arrays and microelectronics for biomedical research and diagnostics

et al. 2010

View full text Add to dashboard Cite

show abstract

“…As opposed to ISFET's, which measure the charge of DNA molecules indirectly, direct charge sensing, usually implemented by induction on capacitive electrodes, is used as well. Sensitivity levels as 2203 e − [40], 100 pM [41] and 10 −14 C (which is roughly 60000 e − ) [42] are reported with this technique. This paper reports a novel CMOS sensor chip utilizing direct charge sensing technique [43] with improved sensitivity compared to its counterparts.…”

Section: Introductionmentioning

confidence: 65%

“…This distance is determined by the thickness of nitride passivation layer and APTES, which are about 1 μm and 20 nm [45], [46] respectively. The overall charge Q induced on the surface by a charge q can be calculated as in (1), for given detector width (w = 7 μm) and separation distance (d = 1 μm) [40].…”

Section: Introductionmentioning

confidence: 99%

Label-Free DNA Detection Using a Charge Sensitive CMOS Microarray Sensor Chip

et al. 2014

View full text Add to dashboard Cite

This paper presents label-free DNA detection using a charge sensitive microarray sensor. The microarray sensor, fabricated with a standard CMOS process, contains 1024 detector elements integrated together with their readout circuit in a single chip. This microarray sensor chip is developed for biosensing applications involving label-free detection of charged particles and molecules with improved sensitivity. The proposed chip is used for the detection of DNA immobilization and hybridization by directly sensing the phosphate backbone charge of DNA molecules. The sensing part of the chip consists of an array of 7 µm × 7 µm capacitive metal electrodes arranged in 32 × 32 format with a pitch of 15 µm, which allows implementation of a portable and low-cost microarray sensor. The sensitivity of the microarray sensor is improved compared with other direct charge sensing CMOS biosensors, using low-noise detection and amplification circuits, and implementing correlated double sampling, which reduces the input referred rms noise level down to 6.8 electrons (e − ). Due to this low noise level, detection of DNA having 1 pM concentration is achieved, showing that the chip is much more sensitive than its counterparts, and even as sensitive as conventional fluorescence or gravimetric detection techniques. A dynamic range of 70 dB is achieved, along with the low noise level. The tests were performed with 10 base pairs DNA in 13-µM probe (5'-TCTCACCTTC-3') and 1-pM target (3'-AGAGTGGAAG-5') oligomer solutions. In both cases, charges of the DNA molecules interacting with the surface were successfully detected.

show abstract

“…The biomolecules are shown to be captured on the top metal layer (marked as TM2) of the back-end-of-line (BEOL) stack of the CMOS process. Complete CMOS biosensors or biochips have then been explored by researchers for various applications, like DNA characterization [ 50 , 51 , 52 ], detection of biomarkers [ 53 ], and cytometric application [ 54 , 55 , 56 ]. The next step is the monolithic integration of microwave sensor architecture on the CMOS/BiCMOS platform.…”

Section: Integrated Microwave Biosensormentioning

confidence: 99%

A Review on Passive and Integrated Near-Field Microwave Biosensors

2017

View full text Add to dashboard Cite

In this paper we review the advancement of passive and integrated microwave biosensors. The interaction of microwave with biological material is discussed in this paper. Passive microwave biosensors are microwave structures, which are fabricated on a substrate and are used for sensing biological materials. On the other hand, integrated biosensors are microwave structures fabricated in standard semiconductor technology platform (CMOS or BiCMOS). The CMOS or BiCMOS sensor technology offers a more compact sensing approach which has the potential in the future for point of care testing systems. Various applications of the passive and the integrated sensors have been discussed in this review paper.

show abstract

A Label-free CMOS DNA Microarray based on Charge Sensing

Abstract: -The first label-free CMOS

Cited by 10 publications

References 12 publications

The potential of microelectrode arrays and microelectronics for biomedical research and diagnostics

The potential of microelectrode arrays and microelectronics for biomedical research and diagnostics

Label-Free DNA Detection Using a Charge Sensitive CMOS Microarray Sensor Chip

A Review on Passive and Integrated Near-Field Microwave Biosensors

Contact Info

Product

Resources

About