A label-free multi-functionalized graphene oxide based electrochemiluminscence immunosensor for ultrasensitive and rapid detection of Vibrio parahaemolyticus in seawater and seafood

Yan, Sha; Zhang, Xuan; Li, Wenrou; Wu, Wei; Wang, Sui; Guo, Zhiyong; Zhou, Jun; Su, Xiurong

doi:10.1016/j.talanta.2015.09.058

Cited by 57 publications

(21 citation statements)

References 44 publications

(47 reference statements)

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“…Its reproducibility capacity plays an extremely important role in practical application for a biosensor. With this device, the relative standard deviation (RSD) toward the target DNA concentration of 4.33% (n = 6) was an excellent result in terms of the reproducibility and precision of the optimized DNA biosensor and compares well with other attempts such as that of Sha et al ( 2016 ) who reported RSD measurements of 7.8%.…”

Section: Discussionsupporting

confidence: 78%

“…Although most conventional DNA-based biosensors have the advantage of high selectivity due to the unique structure of the probe DNA, the complicated preparation process and the low electrochemical signal intensity discourage many attempts at practical application (Wang et al 2016 ). Additionally, many efforts suffer from one or more other drawbacks including long analytical time, high analytical costs and expensive instrumentation (Sha et al 2016 ).…”

Section: Discussionmentioning

confidence: 99%

“…Recent examples which show promise include a highly selective and sensitive SERS-based aptasensor which used Au@Ag core–shell nanoparticles as the active substrate (Duan et al 2016 ) which needs to be more sensitive and a label-free electrochemiluminescence (ECL) immunosensor based on multi-functionalized graphene oxide but which needs to be tested in the field (Sha et al 2016 ).…”

Section: Discussionmentioning

confidence: 99%

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A simple, portable, electrochemical biosensor to screen shellfish for Vibrio parahaemolyticus

et al. 2017

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An earlier electrochemical mechanism of DNA detection was adapted and specified for the detection of Vibrio parahaemolyticus in real samples. The reader, based on a screen printed carbon electrode, was modified with polylactide-stabilized gold nanoparticles and methylene blue was employed as the redox indicator. Detection was assessed using a microprocessor to measure current response under controlled potential. The fabricated sensor was able to specifically distinguish complementary, non-complementary and mismatched oligonucleotides. DNA was measured in the range of 2.0 × 10−8–2.0 × 10−13 M with a detection limit of 2.16 pM. The relative standard deviation for 6 replications of differential pulse voltammetry (DPV) measurement of 0.2 µM complementary DNA was 4.33%. Additionally, cross-reactivity studies against various other food-borne pathogens showed a reliably sensitive detection of the target pathogen. Successful identification of Vibrio parahaemolyticus (spiked and unspiked) in fresh cockles, combined with its simplicity and portability demonstrate the potential of the device as a practical screening tool.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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A simple, portable, electrochemical biosensor to screen shellfish for Vibrio parahaemolyticus

et al. 2017

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“…The most frequent bacterial targets for biosensors are foodborne pathogens including Escherichia coli [13,14,15], Salmonella enterica [16,17,18], Listeria monocytogenes [19] and Vibrio parahaemolyticus [20]. The EIS biosensors for bacteria provide typical limits of detection (LODs) of 10 2 -10 4 CFU mL À 1 (colony-forming units) with no pre-enrichment and analysis time around 1-2 hours [19,21,22].…”

Section: Introductionmentioning

confidence: 99%

Amperometric Immunosensor for Rapid Detection of Honeybee Pathogen Melissococcus Plutonius

et al. 2019

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European foulbrood (EFB) is a honeybee larvae disease caused by a bacterium Melissococcus plutonius. An amperometric immunosensor based on a sandwich assay was developed for rapid point‐of‐care detection of this pathogen. An in‐house made anti‐Melissococcus antibody was immobilized to a gold surface of a screen‐printed sensor via self‐assembled monolayer of cysteamine activated with glutaraldehyde. The direct impedimetric detection of captured microbial cells was tested, however, a better performance was obtained after the formation of sandwich with the peroxidase‐labeled antibody in the amperometric mode. The label‐free assay was limited by higher non‐specific binding. The limit of detection of the immunosensor was 6.6×104 CFU mL−1 (colony‐forming units) with wide linear range between 105 CFU mL−1 and 109 CFU mL−1. The whole analysis was completed within 2 h, which is shorter compared to common laboratory diagnostic tools, such as enzyme‐linked immunosorbent assay or polymerase chain reaction. Furthermore, atomic force microscopy was used for confirmation of the bacteria presence on the electrode surface. The developed immunosensor was successfully employed in the analysis of real samples of honeybees and larvae. The achieved results demonstrate the potential of the amperometric immunosensor for practical in‐field diagnosis of EFB, which can prevent infection spreading and connected losses of honeybee colonies.

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“…Various approaches have been developed to obtain better performances of VP analysis, including enzyme-linked immunesorbent assay (ELISA), [8][9][10] DNA probe, 11 most probable number (MPN), 12,13 polymerase chain reaction (PCR), [14][15][16][17][18] loop-mediated isothermal amplification (LAMP), [19][20][21][22][23] and electrochemistry (EC). 24,25 Despite each of these new approaches having a combination of excellent sensitivity, accuracy and specificity, they still suffer drawbacks involving high analytical cost, need for expensive equipment, and professional trained personnel. Therefore, simple, rapid, sensitive and low-cost assays for determining VP are urgently needed.…”

Section: Introductionmentioning

confidence: 99%

Christmas-tree Derived Amplification Immuno-Strategy for Sensitive Visual Detection of Vibrio parahaemolyticus Based on Gold Label Silver Stain Technology

Song

et al. 2017

ANAL. SCI.

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Recently, Liu and his coworkers designed a Fe(III)-grafted g-C3N4 photocatalyst that displayed an excellent photocatalytic performance. 37 These make it promising in the development of biosensors with high 2017 © The Japan Society for Analytical Chemistry † To whom correspondence should be addressed. A developed Christmas-tree derived immunosensor based on a gold label silver stain (GLSS) technique was fabricated for a highly sensitive analysis of Vibrio parahaemolyticu (VP). In this strategy, captured VP antibody (cAb) was immobilized on a solid substrate; then, the VPs were sequentially tagged with a signal probe by incubating the assay with a detection VP antibody (dAb) conjugated gold nanoparticles (AuNPs)-labeled graphite-like carbon nitride (g-C3N4). Finally, the attached signal probe could harvest a visible signal by the silver meal deposition, and then followed by homebrew Matlab 6.0 as a grey value acquisition. In addition, the overall design of the biosensor was established in abundant AuNPs and g-C3N4 with a two-dimensional structure, affording a bulb-decorated Christmas-tree model. Moreover, with the optimized conditions, the detection limit of the as-proposed biosensor is as low as 10 2 CFU (Colony-Forming Units) mL -1 , exhibiting an increase of two orders of magnitude compared with the traditional immune-gold method. Additionally, the developed visible immunosensor was also successfully applied to the analysis of complicated samples.

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A label-free multi-functionalized graphene oxide based electrochemiluminscence immunosensor for ultrasensitive and rapid detection of Vibrio parahaemolyticus in seawater and seafood

Cited by 57 publications

References 44 publications

A simple, portable, electrochemical biosensor to screen shellfish for Vibrio parahaemolyticus

A simple, portable, electrochemical biosensor to screen shellfish for Vibrio parahaemolyticus

Amperometric Immunosensor for Rapid Detection of Honeybee Pathogen Melissococcus Plutonius

Christmas-tree Derived Amplification Immuno-Strategy for Sensitive Visual Detection of Vibrio parahaemolyticus Based on Gold Label Silver Stain Technology

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