A large indel mutation of the bovine ADD1/SREBP1c gene and its effects on growth traits in some native cattle breeds from China

Abstract

Adipocyte determination and differentiation-dependent factor 1/sterol regulatory element-binding protein-1c (ADD1/SREBP1c) is a major determinant of tissue differential lipogenic capacity in mammalian and avian species. The objectives of the present study were to focus on insertion-deletion polymorphism (indel) in the bovine ADD1/SREBP1c gene, and analyzing its effect on growth traits in a sample of 1035 cattle belonging to four Chinese cattle breeds. PCR-SSCP, DNA sequencing and agarose electrophoresis methods were used. The 778 bp PCR products of ADD1/SREBP1c gene exhibited three genotypes and two alleles were revealed: W and D. Frequencies of the W allele varied from 0.8651 to 1.000. The associations of the 84 bp indel mutation of ADD1/SREBP1c gene with growth traits of 265 Nanyang cows were analyzed. The animals with genotype WD had significantly higher birth weight, body weight, average daily gain than those with genotype WW at birth, 6-, 12-, 18-, and 24-month old (P < 0.05 or P < 0.01). These results suggest that the indel mutation of bovine ADD1/SREBP1c gene may influence the growth traits in cattle.

Effect of a genetic polymorphism in SREBP1 on fatty acid composition and related gene expression in subcutaneous fat tissue of beef cattle breeds

“…The frequency of S allele in Salers bulls was 0.385, higher than Pirenaica heifers (0.214) and bulls (0.135). In contrast, all Holstein‐Friesian individuals showed LL genotype as already reported (Huang et al., 2011; Kaneda et al., 2011; Proskura, 2013) which could have been the consequence of a high selection pressure toward milk production. The scientific literature shows that beef breeds seem to have higher S allele frequency (0.450; Kaneda et al., 2011) compared to dairy breeds (0.160; Conte et al., 2010).…”

“…This polymorphism has been widely investigated in cattle breeds such as Holstein‐Friesian, Hereford and Angus, as well as in Bos indicus and other local breeds (Kaneda et al., 2011; Öztabak et al., 2013; Proskura, 2013). The 84 bp‐indel has also been associated with growth traits (Huang et al., 2011) and milk production (Proskura et al., 2017), while some other studies have investigated its association with FA composition of backfat and muscle (Barton et al., 2010; Han et al., 2013; Lee et al., 2013; Matsuhashi et al., 2011; Xu et al., 2013). However, there are no studies investigating the presence/absence of 84 bp‐indel along with SREBP1 gene expression and their association with the FA profile.…”

“…The 84 bp-indel has also been associated with growth traits (Huang et al, 2011) and milk production (Proskura et al, 2017), while some other studies have investigated its association with FA composition of backfat and muscle (Barton et al, 2010;Han et al, 2013;Lee et al, 2013;Matsuhashi et al, 2011;Xu et al, 2013). However, there are no studies investigating the presence/absence of 84 bp-indel along with SREBP1 gene expression and their association with the FA profile.…”

Association of a polymorphism in SREBP1 with fatty acid composition and lipogenic gene expression in beef cattle breeds

“…The frequency of S allele in Salers bulls was 0.385, higher than Pirenaica heifers (0.214) and bulls (0.135). In contrast, all Holstein-Friesian individuals showed LL genotype as already reported [18,20,21] which could have been the consequence of a high selection pressure towards milk production. The scientific literature shows that beef breeds seem to have higher S allele frequency compared to dairy breeds.…”

“…This polymorphism has been widely investigated in cattle breeds such as Holstein-Friesian, Hereford and Angus, as well as in Bos indicus and other endogenous breeds [18,19,20]. The 84 bpindel has also been associated with growth traits [21] and milk production [22], while some other studies have investigated its association with FA composition of backfat and muscle [23,24,25,26,27]. However, there are no studies investigating the potential effect of 84 bp-indel along with SREBP1 gene expression and their association with the FA profile.…”