A linkage map of chickpea (Cicer arietinum L.) based on populations from Kabuli × Desi crosses: location of genes for resistance to fusarium wilt race 0

Abstract

Two recombinant inbred line (RIL) populations derived from intraspecific crosses with a common parental line (JG62) were employed to develop a chickpea genetic map. Molecular markers, flower colour, double podding, seed coat thickness and resistance to fusarium wilt race 0 (FOC-0) were included in the study. Joint segregation analysis involved a total of 160 markers and 159 RILs. Ten linkage groups (LGs) were obtained that included morphological markers and 134 molecular markers (3 ISSRs, 13 STMSs and 118 RAPDs). Flower colour (B/b) and seed coat thickness (Tt/tt) appeared to be linked to STMS (GAA47). The single-/double-podding locus was located on LG9 jointly with two RAPD markers and STMS TA80. LG3 included a gene for resistance to FOC-0 (Foc0(1)/foc0(1)) flanked by RAPD marker OPJ20(600) and STMS marker TR59. The association of this LG with FOC-0 resistance was confirmed by QTL analysis in the CA2139 x JG62 RIL population where two genes were involved in the resistance reaction. The STMS markers enabled comparison of LGs with preceding maps.

Impact of Genomic Technologies on Chickpea Breeding Strategies

“…An intraspecific chickpea genome map consisting of 51 STMS, 3 inter-simple sequence repeats (ISSRs) and 12 resistance gene analogs (RGA) mapped to eight linkage groups was developed using an F 2 population of chickpea [67]. Another map developed based on a "Kabuli × Desi" cross included a total of 134 molecular markers (3 ISSR, 13 STMSs AND 118 RAPDs) mapped to 10 linkage groups [68]. This map spanned a genomic region of 534.5 cM with an average marker interval of 8.1 cM.…”

Chickpea Improvement: Role of Wild Species and Genetic Markers

“…This work may help in identifying genes responsible for early flowering which may help in developing varieties which may escape terminal drought. QTLs for flower colour, double podding, seed coat thickness, seed weight and carotenoids have been also reported (Cobos et al, 2005;Abbo et al, 2005; using interspecific as well as intraspecific mapping populations, but none of these QTLs have shown very tight linkage with the markers needed for MAS, Furthermore, the results of none QTL mapping experiment have rarely been validated in different crosses to check their robustness (Table 4).…”

Translational Genomics in Agriculture: Some Examples in Grain Legumes

“…Among these QTL clusters, one QTL cluster corresponding to QTLs for 12 traits and explaining about 60% phenotypic variation has been identified on CaLG04, referred to as a "QTL-hotspot" (Varshney et al, 2013b). Similar mapping efforts have been undertaken for important the biotic stresses like FW (Benko-Iseppon et al, 2003;Cobos et al, 2005;Sabbavarapu et al, 2013), and Aschochyta blight (Anbessa et al, 2009;Aryamanesh et al, 2010;Kottapalli et al, 2009). One agronomic trait under complex genetic control (Buckler et al, 2009;Mouradoy et al, 2002) that has received much attention is flowering time.…”

“…In the case of soybean, in addition to developing improved lines or varieties for resistance to different biotic stresses like soybean cyst nematode (SCN) races (Cahill and Schmidt, 2004;Concibido et al, 1996), phytophthora root rot and brown stem rot (Cobos et al, 2005;2009;Hossain et al, 2011;Lichtenzveig et al, 2006) …”