A liquid-junction/low-flow interface for sensitivity improvement in micelle electrokinetic chromatography–electrospray ionization-mass spectrometry

Liu, Yilin; Wang, Nan-Hsuan; Li, Fuan; Her, Guor‐Rong

doi:10.1016/j.chroma.2009.10.049

Cited by 11 publications

(11 citation statements)

References 30 publications

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“…With respect to MS detection, there is only one study concerning the use of this detector for the determination of sulfonamide antibiotics as summarized in Table 4 64. The aim of this study was the evaluation of a liquid‐junction/low‐flow interface for sensitivity improvement in MEKC‐ESI‐MS for sulfadiazine, sulfamerazine, sulfathiazole, sulfamethoxazole, sulfamethoxypyridazine, sulfamethazine, sulfamonomethoxine, sulfadimethoxine, and sulfaquinoxaline.…”

Section: Sulfonamidesmentioning

confidence: 99%

“…The simultaneous determination of antibiotics from different groups was carried out using several CE modes such as CZE, MEKC, and EKC. UV detection was mostly employed although ECL 83 and MS detection was also used 64. The methods were applied to the analysis of biological fluids, foods, and pharmaceutical formulations, as summarized in Table 6.…”

Section: Determination Of Mixtures Of Antibioticsmentioning

confidence: 99%

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Recent advances in the analysis of antibiotics by CE and CEC

et al. 2011

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This article reviews the recent developments achieved in the analysis of antibiotics by CE and CEC. CZE has mainly been employed although other separation modes have also been used. Different detection systems coupled to CE have been reported for antibiotic determination. Enhancement on sensitivity through the use of diverse offline, inline, and online preconcentration approaches is also described. Special attention has been paid to sample treatment steps and to the applications developed in different research areas such as the pharmaceutical, biological, food, and environmental. This manuscript covers the period of time from the previous review published in June 2009 until May 2011.

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Section: Sulfonamidesmentioning

confidence: 99%

Section: Determination Of Mixtures Of Antibioticsmentioning

confidence: 99%

Recent advances in the analysis of antibiotics by CE and CEC

et al. 2011

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“…The SDS and Sulfonamide antibiotics (sulfadiazine, sulfamerazine, sulfathiazole, sulfamonomethoxine, sulfamethoxypyridazine, sulfamethazine, and sulfamethoxazole) were obtained from Sigma-Aldrich Chemical. The ADS was prepared by exchanging the sodium ion with ammonium using a strong acidic cation exchange resin, Amberlyst 15 from Sigma-Aldrich (St. Louis, MO, USA) [14]. The running buffer for the microchip MEKC separation of sulfonamide antibiotics was 40 mM ADS in 20 mM ammonium acetate, pH 7.80.…”

Section: Materials and Sample Preparationmentioning

confidence: 99%

“…Column MEKC with ESI/MS can use either conventional sheath liquid interfaces or low-sheath-flow interfaces [11][12][13]. To alleviate the ion suppression effect, we previously reported the use of a liquid junction/low-flow interface (a double junction interface) to prevent MEKC surfactant from entering the ESI/MS, and thus alleviate the ion suppression effect caused by the presence of surfactant in the capillary [14,15]. In our design, a 5-cm connecting column was used between the liquid junction reservoir and the sheath liquid reservoir.…”

Section: Introductionmentioning

confidence: 99%

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A comparative study of interfaces for microchip micellar electrokinetic chromatography‐electrospray ionization mass spectrometry using the surfactant ammonium dodecyl sulfate

Huang

Her

2011

Electrophoresis

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Using ammonium dodecyl sulfate (ADS) as the surfactant, the response of three common interfaces in the direct coupling of microchip micellar electrokinetic chromatography with electrospray ionization mass spectrometry was studied. In the range of 10-40 mM surfactant, a conventional sheath liquid interface provided poorer sensitivity than both sheathless interface and low-sheath-flow interface. At a surfactant concentration <20 mM, a low-sheath-flow interface exhibited less sensitivity than a sheathless interface; however, it outperformed the sheathless interface above a concentration of 20 mM. At a surfactant concentration above 20 mM, signal reduction due to dilution of the analyte compensated by signal enhancement gained from a reduction in ion suppression effect. The difference in responses of the interfaces was mainly due to the dilution effect, whereas the effect of flow rate became an important factor when the difference in responses between the interfaces was not significant. The utility of the PMMA microchip MEKC/MS using a low-sheath-flow interface was demonstrated by the analysis of sulfonamides at a concentration of 40 mM. The interday precision was in the range of 4.9-14.5%, and the LOD was in the range of 0.34-1.03 ng/mL (MEKC/MS/MS).

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Micellar electrokinetic chromatography: A practical overview of current methodological and instrumental advances

Silva

2010

Electrophoresis

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This review highlights recent methodological and instrumental advances in MEKC focused on the improvement of this CE technique in routine analyses. As in the previous reviews, the present one deals with the last 2 years' most relevant methodological contributions for improving sensitivity and resolution in MEKC as well as instrumental research related to MS and LIF detection. The most widespread approaches are discussed in detail, including enhancements in sensitivity arising from the use of on-line sample concentration (stacking, sweeping, and a combination of both of these protocols) and improvements in resolution, obtained by changing the composition of the BGE (e.g. with additives to the aqueous phase or employing alternative micellar phases to classical SDS) and using 2-D separation systems. The advantages and restrictions in MS and LIF detection as applied to MEKC analysis are also examined, especially in the direct coupling of MEKC and MS detection. Finally, some thoughts on potential future directions are also considered.

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A liquid-junction/low-flow interface for sensitivity improvement in micelle electrokinetic chromatography–electrospray ionization-mass spectrometry

Cited by 11 publications

References 30 publications

Recent advances in the analysis of antibiotics by CE and CEC

Recent advances in the analysis of antibiotics by CE and CEC

A comparative study of interfaces for microchip micellar electrokinetic chromatography‐electrospray ionization mass spectrometry using the surfactant ammonium dodecyl sulfate

Micellar electrokinetic chromatography: A practical overview of current methodological and instrumental advances

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