A Love-wave biosensor using nucleic acids as ligands

Schlensog, Marc Dominic; Gronewold, Thomas M. A.; Tewes, M.; Famulok, Michael; Quandt, Eckhard

doi:10.1016/j.snb.2004.03.015

Cited by 156 publications

(51 citation statements)

References 11 publications

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“…Mass-dependant devices, in addition, are also very useful systems in aptasensors because they offer a label-free system. However, in detection of small organic chemicals, these methods including surface plasmon resonance (SPR), quartz crystal microbalance (QCM) and cantilever methods, are not efficient because the molecular weight of the target chemicals is generally too small to generate an efficient signal (Liss et al, 2002;Minunni et al, 2004;Schlensog et al, 2004;Gronewold et al, 2005;Misono and Kumar, 2005). Herein, we have developed an electrochemical method for 17␤-estradiol detection using a DNA aptamer immobilized on a gold electrode chip.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical detection of 17β-estradiol using DNA aptamer immobilized gold electrode chip

Kim

Jung

Matsuura

et al. 2007

Biosensors and Bioelectronics

249

129

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Section: Introductionmentioning

confidence: 99%

Electrochemical detection of 17β-estradiol using DNA aptamer immobilized gold electrode chip

Kim

Jung

Matsuura

et al. 2007

Biosensors and Bioelectronics

249

129

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“…We have previously developed a prototype love-wave sensor for the quantitative measurements of alpha-human thrombin and the HIV-1 Rev peptide with a detection limit of approximately 75 pg/cm 2 [8], and the RNA/DNA aptamer coated surface acoustic wave (SAW) sensor for investigating the binding of heparin and antithrombin III to immobilized thrombin [9]. The signaling of aptamerprotein interaction was monitored by Jiang et al [10] who used the luminescence signal change of the complex [Ru(phen) 2 (dppz) 2þ ] resulted from its intercalation into a DNA aptamer as a function of the interaction between the aptamer and its target protein.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Sensing of Aptamer‐Protein Interactions Using a Magnetic Particle Assay and Single‐Use Sensor Technology

et al. 2009

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A magnetic particle assay has been designed herein that can report the interactions of DNA aptamers with their cognate protein targets lysozyme (LYS) and human thrombin (THR). Electrochemical sensing of the biomolecular recognition between each aptamer and its target was explored by using a disposable graphite electrode, PGE, in combination with differential pulse voltammetry (DPV). The magnitudes of the oxidation signals of LYS and THR were measured at þ 780 mV and þ 680 mV, respectively, after interaction with the cognate aptamers attached to the surface of magnetic particles. The detection limits estimated for signal to noise ratios above 3.0 correspond to the concentrations of 10.77 mg/mL LYS (769 nM) and 2.00 mg/mL THR (54.5 nM). Our aptamer based approach that combines magnetic particles with a disposable graphite electrode performs well compared to other aptamer-based sensor-formats for quantitative protein detection with respect to sensitivity, selectivity, detection limit, and reproducibility.

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“…The method describes the first label-free detection for arrayed electrodes utilizing electrochemical impedance spectroscopy. Quandt et al [36] designed a Love-wave biosensor array by coupling aptamers to the surface of a Love-wave sensor chip. The sensor chip consists of five single sensor elements and allows label-free, real-time, and quantitative measurements of protein and nucleic acid binding events in concentration-dependent fashion.…”

Section: Aptamer Based Biosensor Arraymentioning

confidence: 99%