A major histocompatibility complex in the toadxenopus laevis (Daudin)

Pasquier, Louis Du; Chardonnens, X; Miggiano, V.

doi:10.1007/bf01564086

Cited by 89 publications

(24 citation statements)

References 20 publications

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“…40, 441, the presence of distinct subpopulations of lymphocytes [44] and memory [45] have been demonstrated in teleost fish which produce a tetrameric IgM as their only immunoglobulin. In Xenopus, an MHC encodes products responsible for acute graft rejection, MLR, some lymphocyte and red blood cell antigens [46], cell-mediated cytotoxic activity [47], and MHC restriction in T-B collaboration for Ig "G" production [43]. We have demonstrated that the cells involved in specific hapten-carrier recognition are indeed B and T cells, respectively, in Xenopus.…”

Section: Discussionmentioning

confidence: 93%

In vitro evidence for T‐B lymphocyte collaboration in the clawed toad, Xenopus

Blomberg¹,

Bernard²,

Pasquier³

1980

Eur J Immunol

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Requirements for an in vitro secondary antibody response to the soluble antigens dinitrophenylated keyhole limpet hemocyanin and dinitrophenylated flow gamma-globulin are described for the clawed toad, Xenopus. Priming of both hapten and carrier-specific cells is required in order to obtain good responses to the hapten. The carrier-reactive ("helper") cells do not adhere to nylon wool, are X ray-resistant and surface Ig-negative. The cell adherence and X ray sensitivity characteristics of these Xenopus "T" cells and their ability to generate a mixed lymphocyte response, allogeneic cytotoxic and helper activities, are identical to those of thymus-derived (T) cells in higher vertebrates. The hapten-reactive, antibody-secreting cell precursors are nylon-adherent, X ray-sensitive and surface Ig-positive. In the absence of other distinguishing serological markers for T cells in frogs, and considering the thymus dependency of the helper activity described here, the evidence presented is interpreted as demonstrating the requirement for antigen-specific T-B cell cooperation in response to soluble protein antigens in vitro for Xenopus.

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Section: Discussionmentioning

confidence: 93%

In vitro evidence for T‐B lymphocyte collaboration in the clawed toad, Xenopus

Blomberg¹,

Bernard²,

Pasquier³

1980

Eur J Immunol

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“…Partially inbred MHC homozygous X. laevis frogs with the haplotypef g (Du Pasquier et al 1975), and r (Flajnik 1983) were bred and maintained at the University of Miami School of Medicine (Miami, FL). The X. laevis × X. gilli (LG) frogs are isogenic cloned species hybrids that have been described extensively in other publications Flajulk et al 1991 b).…”

Section: Methodsmentioning

confidence: 99%

Hsp70 genes are linked to the Xenopus major histocompatibility complex

1994

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Some of the inducible forms of the heat shock protein 70 (Hsp70) gene family are encoded in the class III region of the major histocompatibility complex (MHC) of mammals. This study was undertaken to determine whether Hsp70 genes are linked to the MHC of Xenopus, an amphibian last sharing a common ancestor with mammals 300-350 million years ago. Segregation analyses involving seven haplotypes demonstrated the linkage of two or three inducible Hsp70 genes to the frog MHC. Another Hsp70 gene is not closely linked to the MHC. We conclude that the physical association of MHC class I and class II genes with Hsp70 genes is ancient.

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“…RNA was isolated by the guanidinium method (Stratagene) from the liver, thymus, and spleen of a froglet X. laevis homozygous for the f MHC haplotype (17). cDNA was prepared with the Uni-Zap system (Stratagene), which allows for unidirectional cloning of the resultant cDNAs.…”

Section: Methodsmentioning

confidence: 99%

Evolution of the major histocompatibility complex: molecular cloning of major histocompatibility complex class I from the amphibian Xenopus.

Flajnik

Canel

Kramer

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

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Class I major histocompatibility complex (MHC) cDNA clones have been isolated from an expression library derived from mRNA of an MHC homozygous Xenopus laevis. The nucleotide and predicted amino acid sequences show definite similarity to MHC class I molecules of higher vertebrates. The immunoglobulin-like a-3 domain is more similar to the immunoglobulin-like domains of mammalian class II (3 chains than to those of mammalian class I molecules, and a tree based on nucleotide sequences of representative MHC genes is presented.

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A major histocompatibility complex in the toadxenopus laevis (Daudin)

Cited by 89 publications

References 20 publications

In vitro evidence for T‐B lymphocyte collaboration in the clawed toad, Xenopus

In vitro evidence for T‐B lymphocyte collaboration in the clawed toad, Xenopus

Hsp70 genes are linked to the Xenopus major histocompatibility complex

Evolution of the major histocompatibility complex: molecular cloning of major histocompatibility complex class I from the amphibian Xenopus.

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