A metabolite of dietary glucosylceramide from pineapples improves the skin barrier function in hairless mice

Kuwata, Takashi; Hashimoto, Takashi; Ohto, Nobuaki; Kuwahara, Hiroshige; Lee, Jae Won; Bamba, Takeshi; Mizuno, Masashi

doi:10.1016/j.jff.2017.01.017

Cited by 9 publications

(15 citation statements)

References 37 publications

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“…P-GlcCer (purified by HPLC according to Kuwata et al [18]) was kindly donated by Maruzen Pharmaceuticals Co., Ltd (Hiroshima, Japan). L-Hydroxyproline was -purchased from Wako Pure Chemical Industries (Osaka, Japan).…”

Section: Reagentsmentioning

confidence: 99%

“…AD-like symptoms can be induced in these mice by long-term feeding of an unsaturated fatty acids-deficient (HR-AD) diet [17]. Recently, Kuwata et al has reported that not pineapple GlcCer (P-GlcCer) itself but the metabolite ameliorated dry skin symptoms in Hos:HR-1 mice fed HR-AD diet, and P-GlcCer exhibited the improvement of the skin barrier function accompanied with the enhancement of Il23a expression in the small intestine [18]. However, the mechanism of epidermal barrier function improvement by P-GlcCer is not completely understood.…”

Section: Introductionmentioning

confidence: 99%

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Oral administration of pineapple glucosylceramide improves defective epidermal barrier function by restoring diminished level of TGF-β in the skin

Oka

Ohto²,

Kuwahara³

et al. 2020

Eur Food Res Technol

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Section: Reagentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Oral administration of pineapple glucosylceramide improves defective epidermal barrier function by restoring diminished level of TGF-β in the skin

Oka

Ohto²,

Kuwahara³

et al. 2020

Eur Food Res Technol

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“…[8][9][10][11] In addition, glucosylceramide from pineapple is found to show various effects such as skin moisturizing, improvement of atopic dermatitis, and protection of skin barrier . [12][13][14] The wastes from pineapple industry are inedible peels and stems of pineapples. In the past, these wastes are useless or used as feed or fertilizer.…”

Section: Introductionmentioning

confidence: 99%

Composition, enzyme and antioxidant activities of pineapple

Huang

Lin

Liu

et al. 2021

International Journal of Food Properties

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Three pineapple cultivars smooth cayenne, Tainung 17, and Tainung 19 were processed into flesh, peel, and stem. Nine samples were analyzed for their proximate compositions and bromelain activity existed in their water extracts. Pineapple fleshes were rich in reducing sugars and soluble fiber whereas peel and stem were rich in crude fiber. Stem showed higher bromelain activity (102-247.3 U/mg). All 18 samples including water and ethanol extracts were quite effective in the scavenging ABTS + -and DPPH-radical activities and reducing power as evidenced by their low EC 50 (0.98-15 mg/mL). Water extracts of stem were more effective than their ethanol extracts. The scavenging ABTS + -radical ability was highly related with total phenols and flavonoids. Overall, peel and stem of pineapple showed high amounts of reducing sugars and soluble fiber, and higher bromelain activity and exhibit effective antioxidant activities. These wastes of pineapple would be utilized to develop as a sweet supplement or functional product.

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“…Preparation of P-GlcCer and synthesis of 4,8-sphigadienine (4,8-SD) P-GlcCer was prepared as described previously by Kuwata et al 24 The chemical structure of sphingolipid bases varies widely among species, and 4,8-sphigadienine (4,8-SD) (d18:2) is most abundant in plant species. 28 (4Z,8Z)-Sphinga-4,8-dienine (d18:2 4Z,8Z ) was synthesized according to the reported methods.…”

Section: Caco-2/rbl-2h3 Cells Co-culture Systemmentioning

confidence: 99%

“…function. 24 In order to confirm whether GlcCer or 4,8-SD inhibits degranulation, each compound was directly pretreated with RBL cells, and then ⊎-hexosaminidase activity was measured. As shown in Fig.…”

Section: Inhibition Of Type I Hypersensitivity By P-glccer Through Lmir3mentioning

confidence: 99%

Sphingoid base in pineapple glucosylceramide suppresses experimental allergy by binding leukocyte mono‐immunoglobulin‐like receptor 3

Takemura

Ohto²,

Kuwahara³

et al. 2021

J Sci Food Agric

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BACKGROUND The increase in patients suffering from type I hypersensitivity, including hay fever and food allergy, is a serious public health issue around the world. Recent studies have focused on allergy prevention by food factors with fewer side effects. The purpose of this study was to evaluate the effect of dietary glucosylceramide from pineapples (P‐GlcCer) on type I hypersensitivity and elucidate mechanisms. RESULTS Oral administration of P‐GlcCer inhibited ear edema in passive cutaneous anaphylaxis reaction. In a Caco‐2/RBL‐2H3 co‐culture system, P‐GlcCer inhibited β‐hexosaminidase release from RBL‐2H3 cells. The direct treatment of P‐GlcCer on RBL‐2H3 did not affect β‐hexosaminidase release, but sphingoid base moiety of P‐GlcCer did. These results predicted that sphingoid base, a metabolite of P‐GlcCer, through the intestine inhibited type I hypersensitivity by inhibiting mast cell degranulation. In addition, the inhibitory effects of P‐GlcCer on ear edema and degranulation of RBL‐2H3 cells were canceled by pretreatment of leukocyte mono‐immunoglobulin‐like receptor 3 (LMIR3)‐Fc, which can block LMIR3‐mediated inhibitory signals. CONCLUSION It was demonstrated that a sphingoid base, one of the metabolites of P‐GlcCer, may inhibit mast cell degranulation by binding to LMIR3. The oral administration of P‐GlcCer is a novel and attractive food factor that acts directly on mast cells to suppress allergy. © 2021 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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A metabolite of dietary glucosylceramide from pineapples improves the skin barrier function in hairless mice

Cited by 9 publications

References 37 publications

Oral administration of pineapple glucosylceramide improves defective epidermal barrier function by restoring diminished level of TGF-β in the skin

Oral administration of pineapple glucosylceramide improves defective epidermal barrier function by restoring diminished level of TGF-β in the skin

Composition, enzyme and antioxidant activities of pineapple

Sphingoid base in pineapple glucosylceramide suppresses experimental allergy by binding leukocyte mono‐immunoglobulin‐like receptor 3

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