A Method for Quantitative Estimation of Small Amounts of D-Tubocurarine Chloride in Plasma.

Abstract

365enates from normal or tolerant mice after biturates cannot be the sole mechanism rereceiving an L.D.,b0 dose af thiopental. sponsible for barbiturate hypnosis. 4 m It is the observed in Received May 15, 1950. P.S.E.B.M., 1950, ~7 4 vitro d,epression of brain respiration by bar-

pH AS A FACTOR INFLUENCING PLASMA CONCENTRATIONS OF d-TUBOCURARINE

“…"free" drug plus any bound to plasma protein). The d-tubocurarine is extracted from plasma into ethylene dichloride at alkaline pH in the presence of potassium iodide (the method of Quinn and Woislawski, 1950). Subsequently the alkaloid is re-extracted into 0.01 N hydrochloric acid and estimated at 280.5 m(x in the spectrophotometer (in this study the wavelengdi used was 280 m[j.…”

“…Five-ml samples of plasma were added to 10 ml of ethylene dichloride (purified by repeated washings with dilute hydrochloric acid); 1 ml of freshly prepared potassium iodide-glycine buffer (Quinn and Woislawski, 1950) was then added, the mixture shaken by hand for 5 min, transferred to a centrifuge tube and spun at 4,000 r.p.m. for 5 min.…”