A method for the isolation of guanidine

Saunders, John Adamson

doi:10.1042/bj0260801

Cited by 3 publications

(3 citation statements)

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“…blood, this dose being the same as that used for the normal dogs previously reported [Saunders, 1934]. Each animal was bled as completely as possible at a certain time after the injection, and the blood so obtained extracted for guanidine.…”

Section: Methodsmentioning

confidence: 99%

“…If on the other hand parathyroidectomy has resulted in guanidine being produced at an increased rate [Paton, 1924] such that even with the normally efficient mechanism for its removal [Saunders, 1934] guanidine is present in the blood stream at a concentration of 1-2 mg. per 100 ml. [Burns and Sharpe, 1916], then the injection of a relatively large dose of guanidine should again result in a large increase in blood guanidine for some time after the injection.…”

Section: Methodsmentioning

confidence: 99%

“…The present experiments were carried out to find whether guanidine is present in increased amounts in the blood of dogs in tetany following parathyroidectomy, the method of isolation being one against which such criticisms could not be levelled [Saunders, 1932].…”

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Guanidine and the parathyroid glands

Saunders

1935

Biochemical Journal

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Guanidine and the parathyroid glands

Saunders

1935

Biochemical Journal

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Amide

Tauböck

Winterstein²

1933

Spezielle Analyse

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The recovery of guanidine from the blood-stream after injection

Saunders

1934

Biochemical Journal

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IN a previous paper [Saunders, 1932], a method was elaborated for the isolation of guanidine added to shed blood. In the following experiments, the method has been applied to blood from dogs which had been injected with guanidine. It has also been applied to shed blood, with guanidine added to give a concentration equivalent to that produced by the injection. In each case the concentration of guanidine was 90 mg. guanidine per 100 ml. Graphic records of carotid blood pressure were taken throughout each experiment to ascertain whether the action of the drug was typical or not. EXPERIMENTAL.Dogs were anaesthetised with ether 2 hours after the subcutaneous injection of morphine hydrochloride (25 mg. per kg. body weight).Arterial blood pressure was recorded from the right common carotid artery and injections of guanidine were made through a cannula in the left external jugular vein. Each injection was followed at once by 3 ml. 0 9 % sodium chloride solution, to wash in the guanidine. The dose of guanidine was equivalent to 75 mg. base per kg. The guanidine solutions were made from pure guanidine carbonate (A.R.) neutralised with N hydrochloric acid, the excess carbon dioxide being removed by warming.At a definite interval after the injection, the animals were bled as completely as possible from the common carotid artery, and the blood was mixed with half its volume of 1 % sodium oxalate solution. The average volume of blood obtained in 16 experiments was 38 ml. per kg. Of the 16 animals, 12 gave amounts between 35 and 41 ml. per kg., the remaining 4 giving 19, 33, 47 and 54 ml. per kg. The details of one typical experiment were as follows: 18. vii. 33. Dog 4, female, weight 8 kg. 9.0 a.m. 0-2 g. morphine hydrochloride, subcutaneously. 11.0 a.m. Ether. 11.30 a.m. Blood pressure, 91 mm. Hg. Heart rate 196 per minute. 11.31-2 a.m. 0 9 g. guanidine carbonate, neutralised with HCI in 14 ml., 3 ml. saline. B.P. 66 mm. Hg. Heart rate 204 per min. 11.34 a.m. B.P. 83 mm. Hg. Heart rate 165 per minute. 11.38 a.m. Bled from the common carotid artery into 200 ml. 0-6 % sodium oxalate solution. Volume of blood obtained 375 ml. Weight picrate isolated 52 mg. After recrystallisation from water, M.P. was 313-315°. (Correction + 8°) =322°. Microscopically, guanidine picrate.25-2 mg. on analysis gave basic N 14-4 %, picric acid 80-3 %.

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A method for the isolation of guanidine

Cited by 3 publications

References 5 publications

Guanidine and the parathyroid glands

Guanidine and the parathyroid glands

Amide

The recovery of guanidine from the blood-stream after injection

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