A microchip electrophoresis-based fluorescence signal amplification strategy for highly sensitive detection of biomolecules

Qin, Yingfeng; Zhang, Liangliang; Li, Shuting; Zhao, Jingjin; Huang, Yong; Zhao, Shulin; Liu, Yiming

doi:10.1039/c6cc08911a

Cited by 34 publications

(9 citation statements)

References 25 publications

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“…Qin group amplified the fluorescence signal via microchip electrophoresis. The target material was IFN-γ, and the detection limit was 6.5 pM, which showed high sensitivity and specificity [65]. Wang et al induced a fluorescence signal using a hairpin aptamer probe to detect IFN-γ.…”

Section: Fluorescencementioning

confidence: 99%

Recent Advances in Aptasensor for Cytokine Detection: A Review

Kim

Noh

Park

et al. 2021

Sensors

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Cytokines are proteins secreted by immune cells. They promote cell signal transduction and are involved in cell replication, death, and recovery. Cytokines are immune modulators, but their excessive secretion causes uncontrolled inflammation that attacks normal cells. Considering the properties of cytokines, monitoring the secretion of cytokines in vivo is of great value for medical and biological research. In this review, we offer a report on recent studies for cytokine detection, especially studies on aptasensors using aptamers. Aptamers are single strand nucleic acids that form a stable three-dimensional structure and have been receiving attention due to various characteristics such as simple production methods, low molecular weight, and ease of modification while performing a physiological role similar to antibodies.

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Section: Fluorescencementioning

confidence: 99%

Recent Advances in Aptasensor for Cytokine Detection: A Review

Kim

Noh

Park

et al. 2021

Sensors

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“…Although LIF is a highly sensitive detection mode, the small injected sample volumes and narrow structures of the microfluidic chip diminish the detection sensitivity. A fluorescence signal amplification strategy for highly sensitive detection of pM-levels of interferon-gamma by chip electrophoresis-LIF was developed [52]. Hereby, the signal amplification was achieved by a repeated cycling of cleavage and accumulation of the fluorescence reporter.…”

Section: Target Detectionmentioning

confidence: 99%

A decade of microchip electrophoresis for clinical diagnostics – A review of 2008–2017

Wuethrich

Quirino

2019

Analytica Chimica Acta

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A core element in clinical diagnostics is the data interpretation obtained through the analysis of patient samples. To obtain relevant and reliable information, a methodological approach of sample preparation, separation, and detection is required. Traditionally, these steps are performed independently and stepwise. Microchip capillary electrophoresis (MCE) can provide rapid and high-resolution separation with the capability to integrate a streamlined and complete diagnostic workflow suitable for the point-of-care setting. Whilst standard clinical diagnostics methods normally require hours to days to retrieve specific patient data, MCE can reduce the time to minutes, hastening the delivery of treatment options for the patients. This review covers the advances in MCE for disease detection from 2008 to 2017. Miniaturised diagnostic approaches that required an electrophoretic separation step prior to the detection of the biological samples are reviewed. In the two main sections, the discussion is focused on the technical setup used to suit MCE for disease detection and on the strategies that have been applied to study various diseases. Throughout these discussions MCE is compared to other techniques to create context of the potential and challenges of MCE. A comprehensive table categorised based on the studied disease using MCE is provided. We also comment on future challenges that remain to be addressed.

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“…Microchip electrophoresis LED-induced uorescence (MCE-LIF) detection can realize efficient nucleic acid separation and has on-line uorescent labeling capacity, which has been proven to be a powerful tool for nucleic acid analysis. [23][24][25] Our group has developed several sensitive bacterial nucleic acid detection methods that combine nucleic acid amplication with MCE-LIF detection. [26][27][28] Here, we make full use of the advantages of MCE-LIF detection and the CRISPR-Cas12a system, and propose a transcription amplication-derived real-time crRNA generation CRISPR-Cas12a method (TA-CRISPR-Cas12a) combined with MCE-LIF detection for bacterial 16S rDNA biosensing.…”

Section: Introductionmentioning

confidence: 99%

“…Microchip electrophoresis LED-induced fluorescence (MCE-LIF) detection can realize efficient nucleic acid separation and has on-line fluorescent labeling capacity, which has been proven to be a powerful tool for nucleic acid analysis. 23–25 Our group has developed several sensitive bacterial nucleic acid detection methods that combine nucleic acid amplification with MCE-LIF detection. 26–28…”

Section: Introductionmentioning

confidence: 99%