A Microcolorimetric Method for the Determination of Inorganic Phosphorus

Taussky, Hertha H.; Shorr, Ephraim

doi:10.1016/s0021-9258(18)66180-0

Cited by 2,377 publications

(210 citation statements)

References 13 publications

Supporting

Mentioning

207

Contrasting

Unclassified

Order By: Relevance

“…Finally, to calculate the absolute activity of the Na + /K + -ATPase, a phosphate calibration curve ranging from 0.2 to 1.2 mM KH 2 PO 4 was run on each plate, ouabain inhibition of the samples was analysed, and phosphate released (nmol/μl) was determined at 700 nm according to a previously described photometric method 41 . The activity of the Na + /K + -ATPase of each sample can be determined as the difference in the amount of phosphate released between the non-inhibited control (all ATPases active) and a reaction in which only the contaminating ATPases are active (a buffer system without KCl 42 ).…”

Section: Egg Lethality Assaymentioning

confidence: 99%

Genome editing retraces the evolution of toxin resistance in the monarch butterfly

Karageorgi

Groen

Sumbul

et al. 2019

Nature

157

175

View full text Add to dashboard Cite

Author contributions M.K. co-designed and implemented the overall strategy for the creation of the knock-in fly lines, designed and implemented the bioassays, the RT-qPCR experiments and the RMO analysis, performed statistical analyses and co-wrote the manuscript. S.C.G. designed and implemented the overall strategy for the creation of the knock-in fly lines, prepared the sequence data and metadata for the phylogenetic analyses, co-designed all other experiments, and co-wrote the manuscript. F.S. performed the structural modelling and docking site analyses. J.N.P. performed the phylogenetic, ancestral state and co-evolutionary analyses. K.I.V. conducted crosses, genotyping, and feeding experiments, and co-designed the qPCR experiments. J.M.A. and S.L.B. conducted crosses and genotyping, and feeding and sequestration experiments. A.P.H. performed the in vitro physiological experiments and sequestration analyses. T.M. conducted feeding experiments M.A. performed the RMO analysis with M.K., and conducted genotyping and feeding experiments. G.G. completed the RMO and ouabain dietary survival analyses. F.R. supervised the structural modelling and docking site analyses. S.D. oversaw and interpreted in vitro cell line analyses, helped to design the overall project and co-wrote the manuscript. A.A.A. helped to design the overall project, oversaw the in vitro physiological and sequestration experiments, and co-wrote the manuscript. N.K.W. led the overall collaboration, the project design and its integration, creation of fly lines and statistical analyses, and co-wrote the manuscript. Peer review information Nature thanks Joseph W. Thornton and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.Online content Any methods, additional references, Nature Research reporting summaries, source data, extended data, supplementary information, acknowledgements, peer review information; details of author contributions and competing interests; and statements of data and code availability are available at

show abstract

Section: Egg Lethality Assaymentioning

confidence: 99%

Genome editing retraces the evolution of toxin resistance in the monarch butterfly

Karageorgi

Groen

Sumbul

et al. 2019

Nature

157

175

View full text Add to dashboard Cite

show abstract

“…The plant material used consists of clone GT 1 of Hevea brasiliensis (Mueller Argoviensis Euphorbiaceae), belonging to the vegetative growth classes [17,18] and of moderate metabolic activity [19]. Its choice has been motivated by its hardiness, which makes it the most planted clone in the country, giving it a good and stable stand of tippable trees [20].…”

Section: Plant Materialsmentioning

confidence: 99%

Relationship between the Intensity of Latex Harvesting and the Tapping Panel Dryness Expression of Clone GT 1 of Hevea brasiliensis Muell Arg in South-East Côte d'Ivoire

Obouayeba

Djézou

Moussa

et al. 2021

JABB

View full text Add to dashboard Cite

Tapping panel dryness is an important limiting factor in rubber productivity of Hevea brasiliensis. In order to assess the sensitivity to this syndrome, the effect of two intensive latex harvesting technologies on moderately metabolized GT1 clone, has been studied in the South-Eastern region of Côte d'Ivoire. The rubber trees were planted according to the experimental mono-tree device, "One tree one plot design" a tree constituting a repetition, and 31 trees per treatment, selected on circumference and health status criteria. The parameters measured were rubber production, circumference increase, physiological profile and tapping panel dryness sensitivity. The results showed that induction of tapping panel dryness in latex harvesting system, S/2 d/3 6d/7 ET 10 % Pa 1 (1) 1/ w) was significantly low (0.18 ± 0,22 %) than that of the control (S d/1 6d/7 unstimulated, 1.43 ± 1.45 %). Productivity of this particular system was also good (62 ± 16.32 g.a-1.s-1) and it especially has less stress that can cause physiological fatigue, or even the notch dry. Otherwise, Rubber production, radial vegetative growth, physiological parameters of the latex and the tapping panel dryness rate were influenced by the two treatments applied to GT 1. In addition, the medium and high sucrose contents (16.5 ± 3.01%) and thiol group (0.51 ± 0.13 mmol.l-1) of the latex in the treatment (S/2 d/3 6d/7 ET 10% Pa 1 (1) 1 / w), were instrumental in the response to this stimulation. The sensitivity to the tapping panel dryness is in very close linear relation with the harvest intensity of the latex to which the GT 1 clone has been subjected. These results corroborate and confirm the moderate sensitivity to the tapping panel dryness of GT 1 clone.

show abstract

“…Serum calcium and inorganic phosphate were assessed by coloremetricand microcoloremetric method respectively using reagents of Siemens Clinical Laboratory. [28][29] All these biochemical assessment was done in the laboratory of the Biochemistry and Molecular Biology of BSMMU.…”

Section: Data Collectionmentioning

confidence: 99%

Impact of Vitamin D supplementation on Heart Rate Variability in Vitamin D deficient Asthma COPD Overlap Syndrome patients: A Randomized Controlled Trial

Nahar

Ferdousi

Sultana

et al. 2020

J Bangladesh Soc Physiol

View full text Add to dashboard Cite

Background:Vitamin D3 deficiency is common in Asthma COPD overlap syndrome (ACO) and administration of vitamin D3 increased heart rate variability in healthy subjects. Objective: This randomized clinical trial aimed to investigate the therapeutic effect of vitamin D3administration on time domain measures of heart rate variability in 51 male vitamin D3(<30- 10ng/ml) deficient ACO patients. Methods: Patients were given either vitaminD3 capsule or placebo per week orally for 3 months and serum vitamin D3 level and time domain parameters of heart rate variability were assessed before and after the intervention. For statistical analysis, independent and paired sample t test was used. Results: Before intervention, vitamin D3 level and time domain parameters of heart rate variability were similar in all patients but these outcome measures significantly increased in vitamin D3 treated but not in placebo treated patients after 3 months. Conclusion:In conclusion, vitaminD3therapy is effective to improve heart rate variability in vitamin D3 deficient Asthma COPD overlap syndrome. J Bangladesh Soc Physiol. 2020, December; 15(2): 98-107

show abstract

A Microcolorimetric Method for the Determination of Inorganic Phosphorus

Cited by 2,377 publications

References 13 publications

Genome editing retraces the evolution of toxin resistance in the monarch butterfly

Genome editing retraces the evolution of toxin resistance in the monarch butterfly

Relationship between the Intensity of Latex Harvesting and the Tapping Panel Dryness Expression of Clone GT 1 of Hevea brasiliensis Muell Arg in South-East Côte d'Ivoire

Impact of Vitamin D supplementation on Heart Rate Variability in Vitamin D deficient Asthma COPD Overlap Syndrome patients: A Randomized Controlled Trial

Contact Info

Product

Resources

About